Detailed information NTG0075711

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KI243_RS16505
• Genome accession: NZ_AP021904
• Coordinates: 3773631..3774800 (-)
• Length: 389 a.a.
• NCBI ID: WP_042936475.1
• Uniprot ID: A0A7Y7XW81
• Organism: Pseudomonas sp. Ost2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3768631..3779800

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KI243_RS16490 (PHLH7_32590)	-	3769609..3771090 (+)	1482	WP_212809357.1	mannitol dehydrogenase family protein	-
KI243_RS16495 (PHLH7_32600)	xylB	3771121..3772611 (+)	1491	WP_212809359.1	xylulokinase	-
KI243_RS16500 (PHLH7_32610)	-	3772618..3773556 (+)	939	WP_177082641.1	carbohydrate kinase	-
KI243_RS16505 (PHLH7_32620)	pilU	3773631..3774800 (-)	1170	WP_042936475.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KI243_RS16510 (PHLH7_32630)	-	3774936..3777470 (+)	2535	WP_177131411.1	PAS domain-containing protein	-
KI243_RS16515 (PHLH7_32640)	-	3777529..3777804 (+)	276	WP_177107497.1	peptidylprolyl isomerase	-
KI243_RS16520 (PHLH7_32650)	-	3777924..3778850 (-)	927	WP_212809361.1	sugar kinase	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 43163.33 Da
• Isoelectric Point: 6.4393

>NTDB_id=75711 KI243_RS16505 WP_042936475.1 3773631..3774800(-) (pilU) [Pseudomonas sp. Ost2]
MEIHALLKTLAAQDGSDLYLSTGAPPCAKFNGVLKPLGTEALKAGEVALIADGIMDAEQRQQFERELEMNLAMSLPGVGR
FRVNIFKQRNEVSIVARNIKLEIPRFEDLKLPPILLETIMEKRGLVLFVGATGSGKSTSLAALIDYRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLQAFVSQRLVRTTDGKRRAAVEVMLGTPTIRDFIKRNEFSELKGIMEKSEISGMQTFDTAL
FHLVVEGAIDEEEALKNADSVNNLRLRLKLHQDNGALAQQNTPPAPPVAARELDVKDWGLVDDGERGPG

Nucleotide

• Length: 1170 bp

>NTDB_id=75711 KI243_RS16505 WP_042936475.1 3773631..3774800(-) (pilU) [Pseudomonas sp. Ost2]
ATGGAAATCCACGCGTTATTGAAAACCCTGGCCGCCCAGGACGGTTCGGACCTGTACCTGTCGACCGGGGCGCCACCGTG
TGCCAAGTTCAACGGCGTGCTCAAGCCATTGGGCACCGAAGCCCTCAAGGCCGGGGAGGTGGCGCTGATCGCCGACGGTA
TCATGGACGCCGAGCAGCGCCAGCAGTTCGAGCGGGAATTGGAGATGAACCTGGCGATGTCATTGCCGGGTGTCGGGCGT
TTCCGGGTCAATATCTTCAAGCAGCGCAACGAGGTCTCGATTGTCGCCCGTAACATCAAGCTGGAGATTCCCCGCTTCGA
GGACCTCAAGCTGCCGCCGATCCTGCTGGAAACCATCATGGAGAAGCGTGGCCTGGTGCTGTTCGTCGGCGCCACGGGCT
CAGGCAAGTCCACCTCCCTGGCGGCCCTGATCGACTACCGCAACCGCCACAGCAGCGGCCATATCATCACCATCGAGGAC
CCGGTGGAGTATATCCATCGGCACAAGAAGTCGATCATCAACCAGCGTGAAGTGGGGGTCGACACCCGCAGCTTCCATGC
GGCACTGAAAAACACCCTGCGCCAGGCTCCGGATGTGATCCTGATCGGCGAGATCCGTGATCGCGAAACCATGGAGCATG
CCCTGGCCTTTGCCGACACCGGGCACCTGGCGATTTCCACCCTGCATGCCAACAACGCCAACCAGGCACTGGACCGGATC
ATCAATTTCTTTCCGGAAGACCGTCGCCCGCAACTGCTCAACGACCTGGGCAACAACCTGCAGGCCTTTGTCTCGCAACG
ACTGGTGCGCACCACCGACGGCAAGCGCCGGGCGGCGGTGGAGGTGATGCTGGGCACGCCGACGATTCGCGATTTCATCA
AGCGCAACGAGTTTTCCGAGCTCAAGGGCATCATGGAGAAGTCCGAGATCAGCGGCATGCAGACCTTCGACACGGCCTTG
TTCCACCTGGTGGTGGAAGGCGCGATCGACGAAGAGGAAGCGCTGAAGAATGCCGACTCGGTGAACAACCTGCGCCTGCG
CCTGAAGTTGCATCAGGACAACGGCGCGCTCGCCCAGCAGAACACACCGCCCGCACCGCCGGTGGCGGCACGGGAGCTGG
ATGTGAAGGATTGGGGCCTGGTGGACGATGGCGAACGCGGTCCGGGTTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-271)

3D structure

• AlphaFold DB: A0A7Y7XW81

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.98	90.231	0.514
pilU	Acinetobacter baylyi ADP1	52.958	91.26	0.483
pilU	Vibrio cholerae strain A1552	50.411	93.83	0.473
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.623	95.373	0.378
pilT	Pseudomonas stutzeri DSM 10701	41.449	88.689	0.368
pilT	Pseudomonas aeruginosa PAK	41.449	88.689	0.368