Detailed information NTG0756623

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: OHI04_RS09250
• Genome accession: NZ_CP110849
• Coordinates: 1867989..1868636 (-)
• Length: 215 a.a.
• NCBI ID: WP_010906131.1
• Uniprot ID: A0A3N6KQH1
• Organism: Lactococcus lactis strain VHProbi V60
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1862989..1873636

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OHI04_RS09225 (OHI04_09225)	atpF	1863058..1863564 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
OHI04_RS09230 (OHI04_09230)	atpB	1863579..1864292 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
OHI04_RS09235 (OHI04_09235)	-	1864337..1864552 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
OHI04_RS09240 (OHI04_09240)	-	1864737..1865513 (-)	777	WP_039115826.1	alpha/beta hydrolase	-
OHI04_RS09245 (OHI04_09245)	comEC	1865798..1868008 (-)	2211	WP_039115828.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
OHI04_RS09250 (OHI04_09250)	comEA	1867989..1868636 (-)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
OHI04_RS09255 (OHI04_09255)	-	1868696..1870057 (-)	1362	WP_039115830.1	ABC transporter permease	-
OHI04_RS09260 (OHI04_09260)	-	1870054..1870986 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
OHI04_RS09265 (OHI04_09265)	-	1871091..1871435 (-)	345	WP_259747958.1	hypothetical protein	-
OHI04_RS09270 (OHI04_09270)	-	1871593..1872156 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
OHI04_RS09275 (OHI04_09275)	-	1872329..1873507 (-)	1179	WP_012898345.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23624.76 Da
• Isoelectric Point: 4.8659

>NTDB_id=756623 OHI04_RS09250 WP_010906131.1 1867989..1868636(-) (comEA) [Lactococcus lactis strain VHProbi V60]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=756623 OHI04_RS09250 WP_010906131.1 1867989..1868636(-) (comEA) [Lactococcus lactis strain VHProbi V60]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGATAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A3N6KQH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386