Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OQB66_RS04090 Genome accession   NZ_CP110807
Coordinates   941886..943580 (-) Length   564 a.a.
NCBI ID   WP_017278649.1    Uniprot ID   -
Organism   Pseudomonas syringae strain MUP17     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 936886..948580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OQB66_RS04055 (OQB66_04055) - 937093..937626 (-) 534 WP_003317783.1 MOSC domain-containing protein -
  OQB66_RS04060 (OQB66_04060) - 937623..938252 (-) 630 WP_003430735.1 DUF1780 domain-containing protein -
  OQB66_RS04065 (OQB66_04065) - 938378..938839 (+) 462 WP_266065728.1 hypothetical protein -
  OQB66_RS04070 (OQB66_04070) yacG 938966..939175 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  OQB66_RS04075 (OQB66_04075) coaE 939172..939795 (-) 624 WP_024643093.1 dephospho-CoA kinase -
  OQB66_RS04080 (OQB66_04080) pilD 939792..940664 (-) 873 WP_017278650.1 prepilin peptidase Machinery gene
  OQB66_RS04085 (OQB66_04085) pilC 940666..941883 (-) 1218 WP_024960620.1 type II secretion system F family protein Machinery gene
  OQB66_RS04090 (OQB66_04090) pilB 941886..943580 (-) 1695 WP_017278649.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OQB66_RS04095 (OQB66_04095) - 943806..944240 (+) 435 WP_024960621.1 pilin -
  OQB66_RS04100 (OQB66_04100) - 945007..945594 (+) 588 WP_266066872.1 DUF433 domain-containing protein -
  OQB66_RS04105 (OQB66_04105) - 945642..946187 (-) 546 WP_003345116.1 DUF1523 family protein -
  OQB66_RS04110 (OQB66_04110) - 946307..947305 (+) 999 WP_266065734.1 ATP-grasp domain-containing protein -
  OQB66_RS04115 (OQB66_04115) - 947360..948499 (+) 1140 WP_266065737.1 trypsin-like peptidase domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62514.89 Da        Isoelectric Point: 6.4532

>NTDB_id=756250 OQB66_RS04090 WP_017278649.1 941886..943580(-) (pilB) [Pseudomonas syringae strain MUP17]
MTDAVLTGLAKQLVLAELLTEPVAQQAYQQARRDKISLVSYLVQHKLVKSLTLAELASDQFGVPFMDLASLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIAISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTVAIDRFFDSDSGLGNLEDVDLGLD
IEPADGKETTLATQNDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIKEGFPEAK
IGTFKIYGPMGCEHCNGGYRGRVGIYEVVKKTAELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=756250 OQB66_RS04090 WP_017278649.1 941886..943580(-) (pilB) [Pseudomonas syringae strain MUP17]
ATGACTGATGCTGTCCTCACTGGCTTGGCCAAACAATTGGTCCTAGCCGAGCTACTTACTGAACCTGTTGCGCAGCAAGC
GTACCAGCAGGCGCGCCGCGACAAGATTTCGTTGGTGAGCTATCTGGTTCAGCACAAACTGGTCAAAAGCCTGACGCTGG
CAGAATTGGCCTCCGATCAGTTCGGCGTGCCGTTCATGGACCTCGCCAGCTTGGACAAGGAAAGCCAGCCTAAGGGGCTG
GTCAGTGAAAAGCTGGTTAGACAGCATCATGCGCTGCCGCTTTGGCGACGCGGCAATAAGCTTTTCATTGCAATCTCGGA
CCCGACTAATCATCAGGCAGTGACAGATATTCAGTTCAGCACAGGTCTGAACACCGAAGCGATTCTGGTTGAAGATGACA
AGCTGACTGTTGCGATAGACCGGTTTTTTGACAGCGATAGCGGTCTGGGCAATCTGGAGGATGTCGATCTGGGCCTGGAT
ATCGAACCGGCGGATGGCAAGGAAACCACTCTAGCTACACAGAATGACGCTGACGATGCCCCGGTGGTGCGCTTCGTCAA
CAAGATGCTTATGGATGCCATCCGACTAGGATCATCCGACTTGCACTTCGAGCCCTATGAAAAGATCTTCCGCGTACGTT
TGCGTACCGATGGCATCCTGCACGAAGTCGCCAGACCGCCGATCCATTTGGCAAACCGTATTGCTGCGCGTCTGAAGGTC
ATGGCCAGCCTCGATATTTCGGAACGACGCAAACCGCAGGACGGAAGGGTCAAGTTGCGGGTGTCCAAAACAAAGGCCAT
CGATTTTCGTATGAATACCTTGCCCACACTGTGGGGCGAAAAGATCGTGATGCGGATCCTCGACCCGACCAGCGCTCAGA
TGGGTATCGATGCGCTGGGCTATGAGCCAGAACAAAAGGCTTTGTATCTGGAGGCGTTGAAACAGCCGCAAGGCATGATT
CTTGTGACCGGCCCCACGGGTTCCGGCAAGACAGTTTCGCTGTATACGGGCCTGAATATTCTCAATACCGTGGACATCAA
CATATCCACAGCCGAAGACCCGGTAGAGATCAACCTGGAAGGTATCAATCAGGTCAACGTCAACCCGCGCCAAGGGTTGG
ACTTTTCTCAGGCACTGCGCGCCTTCCTGCGTCAAGATCCCGACGTAATCATGGTCGGCGAGATTCGCGACCTGGAAACC
GCCGAAATCGCTATTAAGGCGTCGCAAACCGGCCACATGGTGCTCTCAACTCTGCACACCAACAGCGCAGCAGAAACATT
GACTCGACTGCATCATATGGGCGTTGCAGCGTTCAACATTGCCACCGCAATAAACCTGATTATTGCGCAGCGACTGGCGC
GCAAGCTGTGTAGCCATTGCAAGAAAGAACTCGATATTCCTCGCGAGACATTGATTAAAGAAGGCTTTCCAGAGGCGAAG
ATCGGTACGTTCAAGATCTATGGGCCGATGGGCTGCGAGCATTGCAATGGTGGTTATAGAGGCCGAGTCGGCATTTATGA
GGTGGTCAAGAAAACAGCGGAGCTGGAGCGCATTATCATGGAAGAAGGTAACTCACTTGAGATTTCCAGGCAGATGCGCA
AGGACGGCTTCAATGACCTGCGAACCTCGGGCCTGTCAAAAGCCATGCAGGGCATCACCAGCCTTGAAGAAGTCAACCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.672

100

0.58
  pilB Acinetobacter baylyi ADP1

56.36

100

0.566
  pilB Legionella pneumophila strain ERS1305867

55.185

100

0.557
  pilB Vibrio cholerae strain A1552

50.712

99.645

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

50.626

99.113

0.502
  pilB Vibrio campbellii strain DS40M4

49.47

100

0.496
  pilF Neisseria gonorrhoeae MS11

48.485

99.468

0.482
  pilF Thermus thermophilus HB27

39.046

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.469

93.44

0.369