Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OOT55_RS08735 Genome accession   NZ_CP110629
Coordinates   2023640..2025367 (+) Length   575 a.a.
NCBI ID   WP_265368704.1    Uniprot ID   -
Organism   Marinimicrobium sp. C6131     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2018640..2030367
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OOT55_RS08705 (OOT55_08705) - 2018796..2019185 (+) 390 WP_265368698.1 nucleotidyltransferase family protein -
  OOT55_RS08710 (OOT55_08710) hepT 2019175..2019591 (+) 417 WP_265368699.1 type VII toxin-antitoxin system HepT family RNase toxin -
  OOT55_RS08715 (OOT55_08715) - 2019621..2020787 (-) 1167 WP_265368700.1 lipase family protein -
  OOT55_RS08720 (OOT55_08720) - 2020788..2021294 (-) 507 WP_265368701.1 DUF6795 domain-containing protein -
  OOT55_RS08725 (OOT55_08725) - 2021625..2022137 (-) 513 WP_265368702.1 protease complex subunit PrcB family protein -
  OOT55_RS08730 (OOT55_08730) - 2022699..2023055 (-) 357 WP_416141001.1 pilin -
  OOT55_RS17850 - 2023070..2023184 (-) 115 Protein_1705 pilin -
  OOT55_RS08735 (OOT55_08735) pilB 2023640..2025367 (+) 1728 WP_265368704.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OOT55_RS08740 (OOT55_08740) pilC 2025373..2026647 (+) 1275 WP_265368705.1 type II secretion system F family protein Machinery gene
  OOT55_RS08745 (OOT55_08745) pilD 2026658..2027521 (+) 864 WP_265368809.1 prepilin peptidase Machinery gene
  OOT55_RS08750 (OOT55_08750) coaE 2027525..2028127 (+) 603 WP_265368706.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62521.27 Da        Isoelectric Point: 5.2176

>NTDB_id=755449 OOT55_RS08735 WP_265368704.1 2023640..2025367(+) (pilB) [Marinimicrobium sp. C6131]
MNTSAPALSGLARRLVHDGLLDSDLASQAQAQAARDRVPFVQHVVTHQILDASTVARVAADEFGTPVFDLDSLSTTAIPT
KLVDQKLIQKHHTLPLMKRGNRLFLAVADPTNLHALDEIKFNTGLNTDPILVEADKLAAAIDRYVNAQEESIGDALGGLD
DDALDGLETEGGEPEAPKDDGGKEEDEAPIVRFINKILLDAIKGGASDIHFEPYEKAYRVRFRTDGILQEVAKPPINLAT
RLAARLKVMSQMDISERRVPQDGRIKMKISKTRAIDFRVNTLPTLFGEKVVLRILDPSSAKMGIDALGYEDYQKQLYMDA
LEQPQGMILVTGPTGSGKTVSLYTGLNILNTSERNISTAEDPVEINLEGINQVQVNTRVGLTFAESLRSFLRQDPDVVMV
GEIRDLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPAFNVATSVSLIIAQRLARRLCKSCKKPATDIPDAVL
SEEGFDEIGIPRSELELYHPVGCSQCTNGYKGRVGVYEVVRITPTIASLIMEGGNSLQIAKAAHEAGFNNLRISALRKAA
QGLTSLEEANRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=755449 OOT55_RS08735 WP_265368704.1 2023640..2025367(+) (pilB) [Marinimicrobium sp. C6131]
ATGAATACCTCTGCACCCGCTCTCAGCGGCCTGGCACGCCGGTTGGTCCACGATGGCCTACTGGATTCCGATTTGGCGAG
CCAGGCTCAGGCCCAGGCGGCACGCGATCGCGTGCCTTTTGTGCAGCATGTGGTCACTCATCAGATTCTTGATGCGTCTA
CAGTGGCCCGGGTGGCTGCGGATGAGTTCGGCACCCCTGTTTTCGACCTCGACAGTCTGTCCACCACCGCGATTCCTACC
AAGTTAGTGGACCAGAAGCTGATTCAGAAACACCATACGCTGCCCCTGATGAAGCGTGGCAACCGGCTGTTTCTGGCGGT
GGCGGACCCGACCAACCTTCACGCACTGGACGAGATCAAGTTCAACACCGGCCTGAACACCGACCCGATTCTGGTGGAGG
CCGACAAGCTGGCCGCCGCTATTGATCGTTATGTCAATGCCCAAGAGGAGTCCATAGGGGACGCTTTGGGCGGATTAGAC
GATGACGCTCTGGATGGCCTGGAAACCGAGGGCGGAGAACCCGAGGCGCCGAAAGATGACGGGGGTAAGGAAGAAGATGA
GGCCCCGATTGTCCGCTTTATCAACAAGATTCTGTTGGACGCCATCAAGGGCGGTGCGTCGGATATCCACTTTGAGCCCT
ACGAAAAGGCCTACCGCGTGCGTTTTCGCACCGACGGCATCCTGCAGGAGGTGGCCAAGCCGCCCATCAATCTGGCGACC
CGCCTGGCGGCACGCCTGAAAGTGATGTCCCAGATGGACATTTCCGAGCGCCGGGTGCCGCAGGATGGCCGGATCAAAAT
GAAAATTTCCAAAACCCGAGCCATCGACTTCCGGGTGAACACTCTGCCTACCCTGTTTGGAGAGAAGGTCGTGCTGCGGA
TTCTCGACCCCAGCTCCGCCAAAATGGGTATTGATGCACTTGGTTACGAGGATTATCAGAAGCAACTTTATATGGACGCT
CTGGAGCAACCGCAGGGCATGATTCTGGTGACCGGGCCGACGGGGTCCGGTAAAACCGTGTCTCTCTATACCGGCCTGAA
TATTCTCAATACCAGCGAGCGAAATATTTCCACCGCCGAAGACCCGGTGGAGATTAACCTGGAGGGGATCAACCAGGTTC
AGGTGAATACCCGGGTGGGACTGACCTTTGCCGAATCCCTGCGCTCCTTCCTGCGTCAGGACCCGGACGTGGTGATGGTG
GGGGAAATCCGGGACCTGGAGACGGCGGAAATCGCCATCAAGGCCGCTCAGACCGGCCACTTGGTGCTCTCCACCCTGCA
CACCAACAGCGCCCCGGAAACGCTGACCCGCCTACTCAATATGGGCGTACCCGCCTTTAACGTGGCCACCAGTGTGAGCC
TGATTATCGCCCAGCGCCTGGCCCGCCGATTGTGCAAAAGTTGCAAGAAGCCCGCAACGGATATTCCCGATGCCGTACTG
AGCGAAGAAGGTTTCGATGAGATCGGAATTCCCCGCAGCGAACTGGAGCTGTACCACCCGGTGGGCTGCTCACAGTGTAC
CAATGGTTATAAGGGTCGTGTCGGGGTCTATGAAGTAGTTCGCATCACACCGACCATCGCCAGCCTTATAATGGAGGGAG
GCAACTCCCTGCAAATTGCCAAAGCCGCCCATGAGGCGGGTTTTAATAATCTGCGGATTTCAGCCCTGCGCAAAGCGGCG
CAAGGGTTGACCAGCCTTGAAGAAGCCAACCGAGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.558

99.652

0.603
  pilB Acinetobacter baylyi ADP1

58.188

99.826

0.581
  pilB Legionella pneumophila strain ERS1305867

56.514

98.783

0.558
  pilB Vibrio parahaemolyticus RIMD 2210633

50.265

98.261

0.494
  pilB Vibrio campbellii strain DS40M4

54.951

89.565

0.492
  pilB Vibrio cholerae strain A1552

55.078

89.043

0.49
  pilF Neisseria gonorrhoeae MS11

47.378

99.478

0.471
  pilB/pilB1 Synechocystis sp. PCC 6803

41.916

87.13

0.365