Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ONS87_RS04360 Genome accession   NZ_CP110416
Coordinates   881679..883397 (-) Length   572 a.a.
NCBI ID   WP_132764186.1    Uniprot ID   A0AA46DEG3
Organism   Caldimonas thermodepolymerans strain DSM 15264     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 876679..888397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ONS87_RS04330 (ONS87_04330) - 877390..877818 (+) 429 WP_132764192.1 NUDIX domain-containing protein -
  ONS87_RS04335 (ONS87_04335) - 877842..878042 (-) 201 WP_132764189.1 DNA gyrase inhibitor YacG -
  ONS87_RS04340 (ONS87_04340) zapD 878059..878814 (-) 756 WP_104358369.1 cell division protein ZapD -
  ONS87_RS04345 (ONS87_04345) coaE 878917..879525 (-) 609 WP_104358370.1 dephospho-CoA kinase -
  ONS87_RS04350 (ONS87_04350) pilD 879545..880402 (-) 858 WP_104358371.1 prepilin peptidase Machinery gene
  ONS87_RS04355 (ONS87_04355) pilC 880412..881632 (-) 1221 WP_104358372.1 type II secretion system F family protein Machinery gene
  ONS87_RS04360 (ONS87_04360) pilB 881679..883397 (-) 1719 WP_132764186.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ONS87_RS04370 (ONS87_04370) ispB 883716..884645 (-) 930 WP_232529566.1 octaprenyl diphosphate synthase -
  ONS87_RS04375 (ONS87_04375) rplU 884930..885241 (+) 312 WP_104358374.1 50S ribosomal protein L21 -
  ONS87_RS04380 (ONS87_04380) rpmA 885278..885535 (+) 258 WP_104358375.1 50S ribosomal protein L27 -
  ONS87_RS04385 (ONS87_04385) - 885646..885840 (+) 195 WP_104358376.1 LiaI-LiaF-like domain-containing protein -
  ONS87_RS04390 (ONS87_04390) cgtA 885837..886937 (+) 1101 WP_104358377.1 Obg family GTPase CgtA -
  ONS87_RS04395 (ONS87_04395) proB 886982..888100 (+) 1119 WP_104358378.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62540.70 Da        Isoelectric Point: 5.3558

>NTDB_id=754903 ONS87_RS04360 WP_132764186.1 881679..883397(-) (pilB) [Caldimonas thermodepolymerans strain DSM 15264]
MDSTLAEAPNPTLSGVARVLVNAGKLNAKTAEELAKQAQEKRISFVSAVIAAGAVSASDLAHTLSNAFSLPLMDLSAVDP
QRLPKGLLENKLSAQYQVVVLGKRGSRLFVGGADPTDQEALERIKFATQLTPEWVIVEHDKLTRMLEATNASASEALENI
VGGEFEFDVSEEDTQQESADVTADVEDAPVVRFLQKMLIDAINMRASDLHFEPYEYNYRVRFRVDGELREIAQPPIAIKD
KLASRIKVISRLDIAEKRVPQDGRMKLKFGNRPIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEKEEKERLLEAI
HRPYGMILVTGPTGSGKTVSLYTCLNLLNQPGVNISTVEDPAEINLPGINQVNVNDKAGLTFAAALKAFLRQDPDIIMVG
EIRDLETADIAIKAAQTGHLVMSTLHTNDAPSTLTRLRNMGVAPFNIASSVILITAQRLARRLCENCKAPATYPREALLR
AGFKEEELDGSWKPYRAVGCSACNNGYKGRVGLYQVMPISEEIQRIILADGSSMDIAAQAQREGVRDLRQAGLLKVKLGV
TTLEEVISVTNE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=754903 ONS87_RS04360 WP_132764186.1 881679..883397(-) (pilB) [Caldimonas thermodepolymerans strain DSM 15264]
ATGGACAGCACACTGGCTGAGGCCCCGAATCCGACCCTGTCTGGCGTTGCGCGGGTGCTCGTGAATGCGGGCAAGCTCAA
CGCAAAGACGGCCGAGGAGCTGGCCAAACAGGCCCAGGAAAAACGCATCAGTTTCGTGTCGGCCGTGATCGCGGCAGGCG
CGGTCTCGGCCAGCGACCTGGCCCACACGCTGTCGAATGCGTTCTCGCTGCCGCTGATGGACCTGTCGGCGGTGGATCCG
CAGCGCCTGCCCAAGGGCCTGCTGGAAAACAAGCTCAGCGCGCAGTACCAGGTGGTGGTGCTGGGCAAGCGCGGCAGCCG
GCTGTTCGTCGGCGGGGCCGACCCCACCGACCAGGAAGCCCTGGAGCGCATCAAGTTCGCGACCCAGCTGACGCCCGAGT
GGGTGATCGTCGAGCACGACAAGCTGACCCGCATGCTGGAGGCGACCAATGCCTCCGCGAGCGAGGCGCTGGAGAACATC
GTCGGCGGCGAGTTCGAGTTCGACGTCTCCGAGGAGGACACCCAGCAGGAGTCGGCCGACGTCACTGCCGACGTCGAGGA
CGCGCCGGTCGTGCGCTTCCTGCAGAAGATGCTGATCGATGCGATCAACATGCGCGCCTCGGACCTGCACTTCGAGCCCT
ACGAGTACAACTACCGGGTGCGCTTCCGCGTCGACGGCGAGTTGCGCGAGATCGCCCAGCCGCCGATCGCGATCAAGGAC
AAGCTGGCCTCGCGCATCAAGGTCATCTCCCGCCTGGACATTGCCGAGAAGCGCGTGCCGCAGGACGGCCGGATGAAGCT
GAAGTTCGGCAACCGGCCGATCGACTTCCGCGTCAGCACGCTGCCCACGCTGTTCGGCGAGAAGATCGTGATCCGTATCC
TGGATCCGTCCAGTGCCAAGCTGGGCATCGACGCGCTCGGCTACGAGAAGGAAGAGAAGGAACGCCTGCTGGAGGCGATC
CACCGCCCCTACGGGATGATCCTCGTGACCGGCCCGACCGGCAGCGGCAAGACCGTGTCGCTGTACACCTGCCTGAACCT
GCTGAACCAGCCGGGCGTGAACATCTCCACCGTGGAAGACCCGGCGGAAATCAACCTGCCCGGGATCAACCAGGTCAACG
TCAACGACAAGGCGGGCCTGACCTTCGCGGCCGCGCTGAAGGCCTTCCTGCGCCAAGATCCGGACATCATCATGGTCGGC
GAGATCCGGGACCTGGAAACCGCCGACATCGCGATCAAGGCCGCCCAGACCGGTCACCTGGTGATGTCGACGCTGCACAC
CAACGACGCACCGTCGACACTGACGCGTCTGCGCAACATGGGGGTGGCCCCCTTCAACATCGCGTCCAGCGTGATCCTGA
TCACGGCGCAACGGCTCGCGCGGCGCCTGTGCGAGAACTGCAAGGCCCCCGCAACGTATCCTCGCGAAGCGCTGTTGCGC
GCCGGCTTCAAGGAAGAGGAACTGGACGGCAGCTGGAAGCCCTACCGGGCGGTCGGTTGCTCGGCCTGCAACAACGGCTA
CAAGGGGCGGGTCGGCCTGTACCAGGTCATGCCGATCTCGGAGGAGATCCAGCGCATCATCCTGGCCGACGGCAGTTCGA
TGGACATCGCGGCGCAAGCCCAGAGGGAGGGCGTGCGCGACCTGCGACAGGCCGGGCTGCTGAAGGTCAAGCTGGGCGTG
ACGACGCTGGAAGAAGTGATTTCCGTCACGAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-152)

Type II/IV secretion system protein (T2SSE)

(196-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.947

98.951

0.544
  pilB Acinetobacter baylyi ADP1

54.867

98.776

0.542
  pilF Neisseria gonorrhoeae MS11

54.982

98.252

0.54
  pilB Legionella pneumophila strain ERS1305867

50.887

98.601

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

46.491

99.65

0.463
  pilB Vibrio cholerae strain A1552

46.607

97.902

0.456
  pilB Vibrio campbellii strain DS40M4

45.633

98.077

0.448
  pilF Thermus thermophilus HB27

37.809

98.951

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.658

92.133

0.365