Detailed information NTG0754901

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ONS87_RS04350
• Genome accession: NZ_CP110416
• Coordinates: 879545..880402 (-)
• Length: 285 a.a.
• NCBI ID: WP_104358371.1
• Uniprot ID: A0A2S5T1W5
• Organism: Caldimonas thermodepolymerans strain DSM 15264
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 874545..885402

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ONS87_RS04315 (ONS87_04315)	slmA	875061..875732 (+)	672	WP_104358413.1	nucleoid occlusion factor SlmA	-
ONS87_RS04320 (ONS87_04320)	pdeM	875813..876466 (+)	654	WP_104358365.1	ligase-associated DNA damage response endonuclease PdeM	-
ONS87_RS04325 (ONS87_04325)	-	876515..877393 (+)	879	WP_104358366.1	ATP-binding protein	-
ONS87_RS04330 (ONS87_04330)	-	877390..877818 (+)	429	WP_132764192.1	NUDIX domain-containing protein	-
ONS87_RS04335 (ONS87_04335)	-	877842..878042 (-)	201	WP_132764189.1	DNA gyrase inhibitor YacG	-
ONS87_RS04340 (ONS87_04340)	zapD	878059..878814 (-)	756	WP_104358369.1	cell division protein ZapD	-
ONS87_RS04345 (ONS87_04345)	coaE	878917..879525 (-)	609	WP_104358370.1	dephospho-CoA kinase	-
ONS87_RS04350 (ONS87_04350)	pilD	879545..880402 (-)	858	WP_104358371.1	prepilin peptidase	Machinery gene
ONS87_RS04355 (ONS87_04355)	pilC	880412..881632 (-)	1221	WP_104358372.1	type II secretion system F family protein	Machinery gene
ONS87_RS04360 (ONS87_04360)	pilB	881679..883397 (-)	1719	WP_132764186.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ONS87_RS04370 (ONS87_04370)	ispB	883716..884645 (-)	930	WP_232529566.1	octaprenyl diphosphate synthase	-
ONS87_RS04375 (ONS87_04375)	rplU	884930..885241 (+)	312	WP_104358374.1	50S ribosomal protein L21	-

Sequence

Protein

• Length: 285 a.a.
• Molecular weight: 30818.55 Da
• Isoelectric Point: 7.9590

>NTDB_id=754901 ONS87_RS04350 WP_104358371.1 879545..880402(-) (pilD) [Caldimonas thermodepolymerans strain DSM 15264]
MNNELWGWWLSPAVLGVLGACIGSFLNVVIHRLPLMLERQWKRDSAEMLGQPVPEDAGEPLSLSRPRSRCPHCGHQIRWH
ENIPVLGWLMLKGRCSSCQARISPRYPLVELLTAALFAATAWKLGPQPVALLWCAAIAVLVALTFIDWDTTLLPDDLTLP
LLWAGLVVAGLGWTIPLSSALWGAVGGYLSLWGVYWLFKLTTGKEGMGYGDFKLLAALGAWLGPAMLLPILVGASVIGTV
VGVVMKASGALREGRYVPFGPFLAGAGIAVFLIGPDAVLAWLGWY

Nucleotide

• Length: 858 bp

>NTDB_id=754901 ONS87_RS04350 WP_104358371.1 879545..880402(-) (pilD) [Caldimonas thermodepolymerans strain DSM 15264]
ATGAACAACGAACTTTGGGGCTGGTGGCTGTCACCGGCCGTCCTGGGCGTGCTGGGCGCCTGCATCGGCAGTTTCCTCAA
CGTCGTGATCCACCGCCTGCCGCTCATGCTCGAGCGGCAGTGGAAGCGTGACAGCGCCGAGATGCTCGGCCAGCCCGTGC
CCGAAGATGCCGGCGAGCCGCTGAGCCTGTCGCGCCCGCGCTCGCGCTGCCCGCACTGCGGGCACCAGATCCGCTGGCAC
GAGAACATCCCGGTGCTCGGCTGGCTGATGCTCAAGGGCCGCTGCTCGAGCTGCCAGGCGCGCATCTCGCCCCGCTACCC
GCTGGTCGAGCTGCTGACGGCCGCGCTGTTCGCCGCCACGGCCTGGAAGCTCGGGCCGCAACCCGTCGCGCTGCTGTGGT
GCGCCGCGATCGCGGTGCTGGTGGCGCTGACCTTCATCGACTGGGACACCACCCTGCTGCCCGACGACCTGACGCTGCCG
CTGCTGTGGGCCGGCCTGGTCGTCGCCGGCCTGGGCTGGACCATCCCGCTGTCGTCGGCCCTGTGGGGCGCGGTGGGGGG
CTACCTCAGCCTCTGGGGCGTGTACTGGCTGTTCAAGCTGACCACCGGCAAGGAAGGCATGGGTTACGGCGACTTCAAGC
TGCTCGCCGCGCTGGGCGCCTGGCTCGGCCCGGCCATGCTGCTGCCCATCCTGGTCGGCGCCTCGGTGATCGGCACCGTG
GTCGGCGTCGTGATGAAGGCCAGCGGCGCCCTGCGCGAGGGGCGCTACGTGCCCTTCGGCCCGTTCCTCGCCGGCGCCGG
CATCGCGGTGTTCCTGATCGGCCCGGACGCCGTGCTCGCCTGGCTGGGCTGGTACTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-123)

• Type IV leader peptidase family (Peptidase_A24) (136-243)

3D structure

• AlphaFold DB: A0A2S5T1W5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.174	96.842	0.505
pilD	Vibrio campbellii strain DS40M4	50	91.228	0.456
pilD	Neisseria gonorrhoeae MS11	48.047	89.825	0.432
pilD	Acinetobacter nosocomialis M2	45.725	94.386	0.432
pilD	Acinetobacter baumannii D1279779	45.725	94.386	0.432