Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OKW95_RS14635 Genome accession   NZ_CP110254
Coordinates   3128840..3129958 (+) Length   372 a.a.
NCBI ID   WP_013198760.1    Uniprot ID   A0AAN0UEB6
Organism   Acinetobacter oleivorans strain S4     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3123840..3134958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OKW95_RS14620 (OKW95_14625) - 3126014..3126856 (-) 843 WP_323739682.1 undecaprenyl-diphosphate phosphatase -
  OKW95_RS14625 (OKW95_14630) - 3126964..3127656 (-) 693 WP_323739683.1 YggS family pyridoxal phosphate-dependent enzyme -
  OKW95_RS14630 (OKW95_14635) pilT 3127783..3128820 (+) 1038 WP_003653239.1 type IV pilus twitching motility protein PilT Machinery gene
  OKW95_RS14635 (OKW95_14640) pilU 3128840..3129958 (+) 1119 WP_013198760.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OKW95_RS14640 (OKW95_14645) fur 3130028..3130465 (-) 438 WP_004794120.1 ferric iron uptake transcriptional regulator -
  OKW95_RS14645 (OKW95_14650) - 3130577..3130975 (+) 399 WP_003653245.1 outer membrane protein assembly factor BamE -
  OKW95_RS14650 (OKW95_14655) - 3131040..3131354 (-) 315 WP_191899702.1 RnfH family protein -
  OKW95_RS14655 (OKW95_14660) - 3131354..3132421 (-) 1068 WP_323739684.1 hypothetical protein -
  OKW95_RS14660 (OKW95_14665) - 3132573..3133028 (-) 456 WP_013198763.1 bacteriohemerythrin -
  OKW95_RS14665 (OKW95_14670) - 3133280..3134128 (-) 849 WP_151821698.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41887.10 Da        Isoelectric Point: 6.6401

>NTDB_id=753387 OKW95_RS14635 WP_013198760.1 3128840..3129958(+) (pilU) [Acinetobacter oleivorans strain S4]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNTKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=753387 OKW95_RS14635 WP_013198760.1 3128840..3129958(+) (pilU) [Acinetobacter oleivorans strain S4]
ATGGATTTTAATGACTTACTCAACCTCATGGTTGAAAAAAAATCATCCGATCTTTTTATTACAGATGGTGTTGCACCATC
TATGAAGATTAACGGACAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGCGAAAAACAGCGCAAAGAATTTGCGGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCT
CGCTTTCGTGTGAGTGCTTTTCAGCAGCGTGACATGCCCGGCATGGTGCTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTTCTTAAAGATTTATCAATGACCAAACGTGGCATCATTATTTTTGTTGGTGCGACAG
GTACAGGTAAATCGACGTCTTTGGCTTCAATGATTAGCTATCGTAACCACAATACTAAAGGTCATATCATTACCATTGAA
GACCCCATCGAGTTTATTCACGAACATGCAGGCTGCATTATTACGCAACGTGAAGTCGGGATCGATACAGATTCATTTGA
AATTGCCTTAAAAAATACTTTGCGACAAGCACCCGATGTCATCTTAATTGGTGAGATCCGATCTCGTGAGGTTATGGACT
ACGCGATTGGTTTCGCTGAAACAGGTCACCTTGTATTGGCCACAATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCATAGTCAACTCTACATGGACTTATCTCTTAACTTAAAGGCCATGATTGCGCA
GCAACTTATTCCAACTCCAGATGGCAATTCACGTCGCGCCGCAATTGAAATTTTAATTAACTCACCATTAATTTCAGATT
ACATTCGTAAAGGCGAAATTCATGAAATTAAAGATCTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAA
GCATTGTTTGATCTCTATAAAGCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAATGATTTACG
TTTGACGATTAAGCTTGCTGAAGAAGGACCTGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACCGACAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

74.868

100

0.761
  pilU Pseudomonas stutzeri DSM 10701

67.925

99.731

0.677
  pilU Vibrio cholerae strain A1552

54.054

99.462

0.538
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.659

97.849

0.398
  pilT Legionella pneumophila strain Lp02

39.645

90.86

0.36
  pilT Legionella pneumophila strain ERS1305867

39.645

90.86

0.36