Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LP095_RS12270 Genome accession   NZ_CP110087
Coordinates   2430711..2432420 (-) Length   569 a.a.
NCBI ID   WP_115368947.1    Uniprot ID   A0A378PRH2
Organism   Moraxella bovis strain SAM109242     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2425711..2437420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LP095_RS12250 (LP095_12250) - 2426728..2427243 (-) 516 WP_264683838.1 hypothetical protein -
  LP095_RS12255 (LP095_12255) metN 2427839..2428885 (+) 1047 WP_115368950.1 methionine ABC transporter ATP-binding protein MetN -
  LP095_RS12260 (LP095_12260) - 2428875..2429564 (+) 690 WP_264677425.1 methionine ABC transporter permease -
  LP095_RS12265 (LP095_12265) - 2429744..2430613 (+) 870 WP_264677426.1 MetQ/NlpA family lipoprotein -
  LP095_RS12270 (LP095_12270) pilB 2430711..2432420 (-) 1710 WP_115368947.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LP095_RS12275 (LP095_12275) tpiA 2432721..2433470 (+) 750 WP_264677427.1 triose-phosphate isomerase -
  LP095_RS12280 (LP095_12280) secG 2433481..2433777 (+) 297 WP_078274629.1 preprotein translocase subunit SecG -
  LP095_RS12285 (LP095_12285) - 2433938..2434426 (-) 489 WP_264683837.1 hypothetical protein -
  LP095_RS12290 (LP095_12290) - 2434588..2436360 (-) 1773 WP_264677429.1 sodium:solute symporter family protein -
  LP095_RS12295 (LP095_12295) - 2436363..2436671 (-) 309 WP_264677430.1 DUF4212 domain-containing protein -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 61966.20 Da        Isoelectric Point: 5.0575

>NTDB_id=752182 LP095_RS12270 WP_115368947.1 2430711..2432420(-) (pilB) [Moraxella bovis strain SAM109242]
MSAPNFGGLAHILVSNGLVAPDAMTKALTEAQQQSMAIVPYLVENKVAPANKIASLLAHEFGDPLFDLNALSPDAVNKEL
VDEKIINQYGALPLFKRGNKLFVGLSDPTRLDAIDAISFNAKLTVETIVVEEDKLKKMIENVYSGGGGLGNLGDVDLSGD
FDGGSGDEDDEGTDVNSGGGEDAPVVKFVNKMLLDAIRMGASDLHFEPYEKSYRVRFRVDGVLQIINRPPVQLAGKIAAR
LKVMSQMDISERRAPQDGRIKLKISKTKAIDFRVNSCPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKELFLEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNTPETNISTAEDPVEINLEGINQVNVNPKVGLTFASALKAFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKKPTNVPKQSLLEMGFT
EEDLANPDNVIYEPVGCSECREGYKGRVGIYEVMKIYPSIARIIMEDGNAIQIKDEAMKHGFRDLRRSGIMKVLQGTTSI
QEMYRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=752182 LP095_RS12270 WP_115368947.1 2430711..2432420(-) (pilB) [Moraxella bovis strain SAM109242]
ATGTCCGCTCCGAATTTTGGTGGTTTGGCACACATTTTGGTGTCAAATGGCTTGGTTGCCCCTGATGCCATGACCAAGGC
TCTGACCGAAGCCCAGCAGCAAAGCATGGCGATTGTGCCATATTTGGTAGAAAACAAAGTTGCCCCTGCAAATAAGATTG
CCAGTCTGCTTGCTCATGAATTTGGCGACCCATTATTTGACCTAAATGCACTGTCTCCTGATGCGGTCAATAAAGAGCTG
GTTGATGAGAAAATCATTAATCAGTATGGGGCATTGCCACTCTTTAAACGGGGTAATAAACTTTTTGTGGGTTTGTCCGA
CCCCACCCGTCTAGATGCCATTGATGCCATCTCCTTTAACGCCAAGCTGACTGTTGAGACCATCGTTGTCGAAGAAGACA
AGCTCAAAAAGATGATTGAGAATGTCTACTCAGGCGGTGGCGGACTGGGCAACCTAGGCGATGTTGACCTGTCAGGGGAC
TTTGATGGTGGGTCAGGTGATGAAGATGATGAAGGTACTGACGTCAATTCAGGCGGTGGTGAAGATGCCCCTGTGGTCAA
GTTCGTTAATAAAATGCTCCTTGATGCCATTCGTATGGGAGCATCGGATTTGCACTTTGAGCCGTATGAGAAGTCTTACC
GTGTGCGTTTTCGTGTGGATGGGGTTTTGCAAATCATTAATCGCCCACCAGTACAACTGGCAGGTAAAATCGCCGCTCGT
CTTAAAGTCATGTCGCAAATGGACATATCCGAACGCCGAGCCCCCCAGGATGGTCGTATTAAGCTAAAAATCTCCAAAAC
AAAAGCCATTGACTTTCGTGTGAACTCCTGCCCTACGCTCTTTGGCGAAAAGCTCGTACTGCGTATTCTTGACCCGTCAT
CAGCGATGCTTGGTATTGATGCACTCGGCTATGAGCCTGACCAAAAAGAGCTGTTCTTAGAAGCTCTTGATAAACCTCAA
GGTATGCTACTTATCACAGGTCCGACAGGTTCGGGTAAAACGGTCTCGCTCTATACTGGTCTTAACATCCTAAATACGCC
CGAGACCAACATCTCTACCGCCGAAGACCCTGTGGAAATTAACTTAGAAGGGATTAACCAAGTAAACGTCAACCCCAAAG
TGGGTCTCACGTTCGCCTCCGCCCTAAAAGCATTCCTACGTCAAGACCCTGACATCGTCATGGTCGGTGAGATTCGTGAT
TTGGAGACTGCCGAGATTGCCATTAAAGCCGCCCAAACCGGACACATGGTATTATCCACCCTGCACACCAACTCCGCTCC
TGAGACCCTAACTCGTCTGCGTAATATGGGCGTGGCATCGTTTAACATTGCCACATCGGTAAACCTAGTGATTGCCCAGC
GTCTGGCTCGCCGTTTGTGCAAAAACTGCAAAAAGCCGACCAACGTACCCAAGCAGAGCCTGCTAGAAATGGGCTTTACC
GAAGAAGACCTTGCCAATCCTGATAACGTCATCTATGAGCCTGTGGGCTGTTCTGAGTGTCGTGAAGGCTACAAGGGGCG
TGTGGGCATCTATGAAGTCATGAAAATCTACCCAAGTATCGCTCGGATTATCATGGAAGACGGTAATGCCATTCAAATCA
AAGACGAAGCGATGAAACACGGCTTTCGTGATTTGAGACGCTCGGGTATCATGAAAGTCTTGCAAGGCACGACCAGTATC
CAAGAGATGTATCGGGTAACTTCCGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-145)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378PRH2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

66.96

100

0.67
  pilB Acinetobacter baylyi ADP1

65.554

100

0.656
  pilB Legionella pneumophila strain ERS1305867

52.575

98.946

0.52
  pilF Neisseria gonorrhoeae MS11

51.163

98.243

0.503
  pilB Vibrio cholerae strain A1552

49.554

98.594

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

48.41

99.473

0.482
  pilB Vibrio campbellii strain DS40M4

47.703

99.473

0.475
  pilB/pilB1 Synechocystis sp. PCC 6803

35.61

100

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.456

99.473

0.373
  pilF Thermus thermophilus HB27

36.3

100

0.366