Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AXW80_RS16280 Genome accession   NZ_CP109908
Coordinates   3463271..3464977 (-) Length   568 a.a.
NCBI ID   WP_005314160.1    Uniprot ID   A2T193
Organism   Aeromonas salmonicida subsp. salmonicida strain J227     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3458271..3469977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXW80_RS16250 (AXW80_16205) mutT 3458881..3459288 (+) 408 WP_005314168.1 8-oxo-dGTP diphosphatase MutT -
  AXW80_RS16255 (AXW80_16210) yacG 3459365..3459559 (-) 195 WP_005314167.1 DNA gyrase inhibitor YacG -
  AXW80_RS16260 (AXW80_16215) zapD 3459569..3460291 (-) 723 WP_005314166.1 cell division protein ZapD -
  AXW80_RS16265 (AXW80_16220) coaE 3460329..3460943 (-) 615 WP_005314164.1 dephospho-CoA kinase -
  AXW80_RS16270 (AXW80_16225) pilD 3460963..3461838 (-) 876 WP_005314162.1 A24 family peptidase Machinery gene
  AXW80_RS16275 (AXW80_16230) - 3461902..3463142 (-) 1241 Protein_3155 type II secretion system F family protein -
  AXW80_RS16280 (AXW80_16235) pilB 3463271..3464977 (-) 1707 WP_005314160.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  AXW80_RS16285 (AXW80_16240) tapA 3464981..3465409 (-) 429 WP_005314158.1 type IVa pilus major pilin TapA -
  AXW80_RS16290 (AXW80_16245) nadC 3465744..3466601 (-) 858 WP_005314155.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AXW80_RS16295 (AXW80_16250) - 3466606..3467076 (-) 471 WP_011898292.1 retropepsin-like aspartic protease -
  AXW80_RS16300 (AXW80_16255) ampD 3467226..3467798 (+) 573 WP_005314151.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AXW80_RS16305 (AXW80_16260) pdhR 3468075..3468839 (+) 765 WP_005314150.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62577.06 Da        Isoelectric Point: 5.4384

>NTDB_id=750556 AXW80_RS16280 WP_005314160.1 3463271..3464977(-) (pilB) [Aeromonas salmonicida subsp. salmonicida strain J227]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLMESEQDALGMEEIDESEI
SELEVSDENSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNITEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRVSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=750556 AXW80_RS16280 WP_005314160.1 3463271..3464977(-) (pilB) [Aeromonas salmonicida subsp. salmonicida strain J227]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCATCCCTGCTCAGTGAGTCCGACTCACAGCGTTA
CCTGAGCCAAGCCAAGGCCCAGCGCAAACCCTTCGTGACATTTCTGATTGAAAACGAGATCCTCGACAGCAAGGCGCTGG
CGGATTTTTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAAAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCAGA
CCCGACCAACGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGCTCCATACCGAAGCCCTGCTGGTGGAAGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGATGGAAAGCGAGCAGGATGCCCTCGGCATGGAGGAGATCGACGAGTCGGAGATC
TCCGAGCTGGAAGTCTCGGACGAAAACTCCCGCCTCGATGAGAGCGTCAATACCGCCGATGACGATGCCCCCATCGTCAA
ATACATCAATAAAATCATGATGGATGCCATCAAGCGCGGCGCCTCGGATCTGCACTTCGAACCCTATGAGACCAAGTACC
GTATCCGCTTTCGTATAGACGGCATACTGCACGAAATAGCGACGCCGCCGGTCAATCTGGCCAACCGCTTTTCGGCCCGC
CTCAAGGTGATGGCGCGACTCGATATCGCAGAGCGACGGCTGCCCCAGGATGGTCGCATCAAGTTAAAGCTGTCGCGCAA
CAAATCCATGGATATGCGGGTCAACACACTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTGGACTCTTCCG
CAGCGCGGCTCAATATCGAACAGCTCGGCTTCGACGACCGGCAGAAGGCACAGTATCTGCGTGCCCTCTCCAAGCCCCAG
GGCATGATACTGGTGACGGGCCCGACCGGCTCTGGCAAGACGGTCTCTCTCTATACCGGCCTCAATATTCTCAACATCAC
AGAGGTGAACATCTCAACCGCCGAAGATCCGGTGGAGATCAACCTGCCAGGCGTCAACCAGGTACAAGTCAATCCCAAGG
CGGGGTTGACCTTCGCCAGCGCCCTGCGATCCTTTCTTCGCCAGGATCCCGACGTTGTGATGGTGGGGGAAATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAAGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCAGC
CGAAACCCTGACCCGGATGATGAACATGGGGGTCCCGGCCTTCAATATCGCCTCTTCGGTGACCCTCATCATGGCCCAGC
GCCTCGCTCGCAAGCTCTGCGATCACTGCAAGGCACCGGAAGTGGTGCCCGAAGCTGAATTGCTGGAGCTGGGCTTCACC
CAGCAGCAGTTGGCCGCCGGCTTGCGCCTGTTCAAGCCAGTAGGCTGCAAAGAGTGCTCGGGGGGCTACAAGGGTCGGGT
CGGTATCTACGAAATCATGCTAATGTCTGAAAACATTGCAAAATTGATTATGCAAGGAGCCAACTCCCTGCAAATTGCAG
CGATTGCTCAAAAAGAGGGGATGCGAACCCTGCGTGTCTCGGGCTTGGAGAAGGCCCGCCTGGGGGTGACCAGCCTGGCA
GAGATCAACCGGGTGACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A2T193

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Acinetobacter baumannii D1279779

55.379

99.824

0.553
  pilB Legionella pneumophila strain ERS1305867

54.24

99.648

0.54
  pilB Vibrio cholerae strain A1552

54.336

99.472

0.54
  pilB Vibrio campbellii strain DS40M4

52.12

99.648

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

46.277

99.296

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373