Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OHM81_RS06065 Genome accession   NZ_CP109857
Coordinates   1336793..1338535 (-) Length   580 a.a.
NCBI ID   WP_338500828.1    Uniprot ID   -
Organism   Delftia tsuruhatensis strain GSK TC1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1331793..1343535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OHM81_RS06035 mutT 1332375..1332881 (+) 507 WP_043789746.1 8-oxo-dGTP diphosphatase MutT -
  OHM81_RS06040 - 1332892..1333146 (-) 255 WP_338499823.1 DNA gyrase inhibitor YacG -
  OHM81_RS06045 zapD 1333146..1333901 (-) 756 WP_016445607.1 cell division protein ZapD -
  OHM81_RS06050 coaE 1333991..1334629 (-) 639 WP_016445608.1 dephospho-CoA kinase -
  OHM81_RS06055 pilD 1334644..1335537 (-) 894 WP_016445609.1 A24 family peptidase Machinery gene
  OHM81_RS06060 pilC 1335537..1336754 (-) 1218 WP_047326532.1 type II secretion system F family protein Machinery gene
  OHM81_RS06065 pilB 1336793..1338535 (-) 1743 WP_338500828.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OHM81_RS06075 - 1338772..1339701 (-) 930 WP_043789750.1 polyprenyl synthetase family protein -
  OHM81_RS06080 rplU 1339932..1340243 (+) 312 WP_012207284.1 50S ribosomal protein L21 -
  OHM81_RS06085 rpmA 1340259..1340516 (+) 258 WP_012207283.1 50S ribosomal protein L27 -
  OHM81_RS06090 cgtA 1340666..1341781 (+) 1116 WP_016451026.1 Obg family GTPase CgtA -
  OHM81_RS06095 proB 1341865..1343013 (+) 1149 WP_016445651.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 62720.80 Da        Isoelectric Point: 5.1506

>NTDB_id=750225 OHM81_RS06065 WP_338500828.1 1336793..1338535(-) (pilB) [Delftia tsuruhatensis strain GSK TC1]
MAVADSSLKNASAVASLPGIGRALISAGKVSQQAAEAAAGKAATNRTSFIAELSVAGTLSALEVAETVSTMFSAPLLDLD
AVDAMRLPQDLLDAKIISTYRVLPLSKRGNRLTVATADPTQQEAAEQIKFTTQLGVDWIIVEADKLERLIAQQSKSASEA
MESYGGSGDFEFGDLKPDEVAEEKDNSAAADVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIAS
PPIAIKEKLASRIKVISRMDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEE
KARLLEAISRPYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKAFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKEPAD
IPREALIDAGYDDADIDGSWVTYRPVGCPACNNGYKGRVGIYQVMPISEEIQRIILQDGSALEIAAQAKREGVRSLRGSG
LHKARQGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=750225 OHM81_RS06065 WP_338500828.1 1336793..1338535(-) (pilB) [Delftia tsuruhatensis strain GSK TC1]
ATGGCTGTTGCTGACTCCTCGCTCAAGAATGCATCCGCTGTCGCATCGCTTCCCGGCATCGGGCGGGCCTTGATTTCAGC
AGGCAAGGTATCGCAACAGGCAGCGGAAGCGGCTGCCGGCAAGGCTGCCACCAATCGCACCTCGTTCATTGCAGAACTGA
GCGTCGCCGGCACCCTCTCTGCCCTGGAAGTGGCGGAGACGGTTTCCACCATGTTCAGCGCGCCGCTGCTCGATCTTGAT
GCGGTCGACGCCATGCGCCTGCCGCAGGATCTGCTGGACGCCAAGATCATCAGCACCTATCGCGTCCTGCCTCTGAGCAA
GCGCGGCAACCGCCTCACGGTGGCCACCGCCGACCCGACGCAGCAGGAAGCCGCCGAGCAGATCAAGTTCACCACCCAGT
TGGGCGTGGACTGGATCATCGTCGAGGCCGACAAGCTGGAGCGGCTGATCGCGCAGCAGAGCAAAAGCGCCAGCGAGGCC
ATGGAAAGCTATGGCGGCAGCGGCGACTTCGAGTTCGGCGACCTCAAGCCTGATGAGGTGGCAGAGGAAAAGGACAACTC
GGCCGCAGCAGACGTCGAAGATGCGCCCATCGTCAAGTTCCTGCACAAAATGCTGCTCGACGCCTTCAACATGCGCGCGT
CTGACTTGCATTTCGAACCCTACGAGCACCAGTACCGCGTGCGCTTTCGCATTGACGGCGAGCTGCGTGAAATTGCATCG
CCGCCCATTGCCATCAAGGAGAAGCTGGCCTCGCGCATCAAGGTGATCTCGCGCATGGACATCTCGGAAAAGCGTGTCCC
GCAGGACGGGCGCATGAAGCTCAAGGTCGGCCCGGACCGCGTCATCGACTTTCGCGTCAGCACGCTGCCCACGCTTTTCG
GCGAGAAGATCGTGATCCGTATCCTTGACCCGAGCTCGGCCAAGATGGGCATTGATGCCCTGGGCTATGAACCCGAGGAG
AAGGCACGCCTGCTTGAAGCCATCAGCCGCCCCTACGGAATGATTCTGGTCACAGGCCCCACCGGCTCGGGCAAGACAGT
GTCGCTGTACACCTGCCTGAACCTGCTGAACAAGGCCGGCGTGAACATCGCCACGGCGGAAGATCCTTCGGAAATCAACC
TGCCCGGCGTCAACCAGGTCAATGTGAACGAAAAGGCCGGCCTCACCTTTGCCACTGCGCTCAAGGCTTTTCTACGCCAG
GATCCCGACATCATCATGGTCGGTGAAATCCGCGATCTGGAAACGGCCGACATCTCGATCAAGGCAGCGCAAACCGGCCA
CTTGGTGCTGTCCACCCTGCACACCAATGACGCACCGACCACGTTGACGCGCATGCGCAACATGGGCATTGCGCCCTTCA
ACATCGCATCGAGCGTGATCCTGATCACCGCCCAGCGCCTGGCCAGGCGCCTGTGCCCTCAATGCAAGGAGCCGGCTGAC
ATTCCGCGGGAGGCCCTGATCGATGCAGGCTACGACGATGCGGACATTGACGGCAGCTGGGTCACCTACAGGCCCGTCGG
CTGTCCGGCATGCAACAACGGCTACAAGGGCCGCGTAGGCATCTATCAGGTGATGCCTATCAGCGAGGAAATCCAGCGCA
TCATCCTGCAGGACGGCAGTGCGCTGGAGATCGCCGCACAGGCCAAGCGCGAAGGCGTGCGGTCGCTGCGTGGATCGGGT
TTGCACAAGGCCAGACAGGGACTGACCTCGCTCGAAGAAGTGCTGGCAGTCACCAATGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(57-153)

Type II/IV secretion system protein (T2SSE)

(203-470)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.888

98.103

0.548
  pilB Acinetobacter baumannii D1279779

56.383

97.241

0.548
  pilF Neisseria gonorrhoeae MS11

52.473

97.586

0.512
  pilB Legionella pneumophila strain ERS1305867

50.264

98.103

0.493
  pilB Vibrio cholerae strain A1552

46.679

96.034

0.448
  pilB Vibrio campbellii strain DS40M4

44.248

97.414

0.431
  pilB Vibrio parahaemolyticus RIMD 2210633

46.617

91.724

0.428
  pilF Thermus thermophilus HB27

39.062

99.31

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.416

83.793

0.364