Detailed information NTG0074915

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KUIN1_RS14730
• Genome accession: NZ_AP020337
• Coordinates: 3328857..3330026 (-)
• Length: 389 a.a.
• NCBI ID: WP_003400837.1
• Uniprot ID: -
• Organism: Pseudomonas sp. KUIN-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3323857..3335026

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KUIN1_RS14715 (KUIN1_28200)	-	3325141..3326205 (+)	1065	WP_057457832.1	microcin C ABC transporter permease YejB	-
KUIN1_RS14720 (KUIN1_28210)	-	3326205..3327227 (+)	1023	WP_003400841.1	ABC transporter permease	-
KUIN1_RS14725 (KUIN1_28220)	-	3327229..3328806 (+)	1578	WP_003400838.1	ABC transporter ATP-binding protein	-
KUIN1_RS14730 (KUIN1_28230)	pilU	3328857..3330026 (-)	1170	WP_003400837.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KUIN1_RS14735 (KUIN1_28240)	-	3330150..3330425 (+)	276	WP_002553334.1	peptidylprolyl isomerase	-
KUIN1_RS14740 (KUIN1_28250)	pgm	3330498..3332144 (-)	1647	WP_152092086.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
KUIN1_RS14745 (KUIN1_28260)	-	3332308..3334635 (-)	2328	WP_152092087.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42577.65 Da
• Isoelectric Point: 6.0641

>NTDB_id=74915 KUIN1_RS14730 WP_003400837.1 3328857..3330026(-) (pilU) [Pseudomonas sp. KUIN-1]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGEVAVIAQGLMDEEQKLEFQRELEMNLAVSMAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKRGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDSAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGAGSLTTPPPAPTGSSTASTAEWGLVDDDAPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=74915 KUIN1_RS14730 WP_003400837.1 3328857..3330026(-) (pilU) [Pseudomonas sp. KUIN-1]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTACCTCTCCACCGGAGCACCACCGTG
CGCCAAGTTCAACGGTGTGCTCAAGCCACTGGGCAGCGAAACCTTCAAGCCGGGGGAGGTCGCAGTCATTGCCCAGGGGC
TTATGGACGAGGAGCAGAAGCTCGAGTTTCAACGCGAACTGGAGATGAACCTCGCCGTTTCTATGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTTATGCAGCGCAACGAAGTGTCCATCGTCGCGCGGAATATCAAACTGGACATCCCGCGTTTCGA
AGACCTGTTCCTGCCGCCCGTGCTGCTCGACGTGATCATGGAAAAGCGCGGTCTGGTGCTGTTTGTCGGGGCCACGGGTT
CGGGCAAGTCGACCTCATTGGCGGCGCTGATCGACTACCGTAACCGCAACGCCAGCGGCCATATCATCACCATTGAGGAC
CCGGTCGAGTTCATCCATCGCCACAAGAAATCGATCGTCAACCAGCGCGAAGTCGGGGTCGATACCCGCAGCTTCCATGC
GGCATTGAAAAACACGCTGCGCCAGGCGCCGGATGTGATTCTGATCGGCGAGATTCGTGATCGCGAGACCATGGAGCACG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACCCTGCACGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAATTTCTTCCCGGAAGAGCGTCGCGCGCAGTTGCTGCATGACCTGGGCAACAACCTCAAGGCATTCGTCTCCCAGCG
ACTGGTCAGAACACCGGATGGCAAGCGCCGCGCGGCGGTGGAAGTGATGATGGGCACGCCGACCATTCGCGACCTGATCC
AGCGCAATGAACTGACCGAACTCAAGGGCATCATGGAGAAGTCCGGCAGCCTCGGTATGCAGACGTTCGACTCGGCGCTG
TTCAATCTGGCCGTCGAAGGGGCGATCAGCGAAGAAGAAGCCCTCAAGAACGCCGACTCGCAGAACAACGTGCGCCTGCG
CCTCAAACTGCACAGTGAAGGCGGCGCCGGGAGCCTCACCACACCGCCGCCGGCCCCGACCGGCAGCAGCACGGCAAGCA
CCGCCGAGTGGGGGCTGGTCGATGATGACGCGCCGGGTCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.287	93.059	0.542
pilU	Vibrio cholerae strain A1552	51.105	93.059	0.476
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.164	98.458	0.386
pilT	Pseudomonas stutzeri DSM 10701	41.108	88.175	0.362
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362