Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   OE648_RS08950 Genome accession   NZ_CP107544
Coordinates   1956856..1958934 (+) Length   692 a.a.
NCBI ID   WP_083354032.1    Uniprot ID   -
Organism   Pseudomonas moraviensis strain EFBE32     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1951856..1963934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OE648_RS08930 (OE648_08935) pilM 1954071..1955135 (+) 1065 WP_042608693.1 pilus assembly protein PilM Machinery gene
  OE648_RS08935 (OE648_08940) - 1955135..1955701 (+) 567 WP_042608694.1 PilN domain-containing protein -
  OE648_RS08940 (OE648_08945) pilO 1955698..1956321 (+) 624 WP_042608695.1 type 4a pilus biogenesis protein PilO -
  OE648_RS08945 (OE648_08950) - 1956318..1956842 (+) 525 WP_039756678.1 pilus assembly protein PilP -
  OE648_RS08950 (OE648_08955) pilQ 1956856..1958934 (+) 2079 WP_083354032.1 type IV pilus secretin PilQ Machinery gene
  OE648_RS08955 (OE648_08960) aroK 1958939..1959457 (+) 519 WP_024011270.1 shikimate kinase AroK -
  OE648_RS08960 (OE648_08965) aroB 1959610..1960710 (+) 1101 WP_065615444.1 3-dehydroquinate synthase -
  OE648_RS08965 (OE648_08970) - 1960721..1962307 (+) 1587 WP_333715968.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74939.13 Da        Isoelectric Point: 5.4106

>NTDB_id=740525 OE648_RS08950 WP_083354032.1 1956856..1958934(+) (pilQ) [Pseudomonas moraviensis strain EFBE32]
MNRIFSTLGFSLWIALVSPMVMAANLKALDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLANRNLD
LGSGNARTATVAEAKDRTRLIVSLTQLAPYSTRVEGNNLFVVVGQGASATAPRPAAVAPRAATATSAPARSAAPRSRSIR
GVDFQRGTEGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAALTGDRTVISVEPSG
TFDYSTFQTDNKLTVSVRPMSVDDLQKRNADRQAYVGDKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNASGVN
GSSSTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=740525 OE648_RS08950 WP_083354032.1 1956856..1958934(+) (pilQ) [Pseudomonas moraviensis strain EFBE32]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGGTGTCACCGATGGTAATGGCGGCCAACCTGAA
AGCGCTGGATGTTGCCGCGCTGCCGGGTGACCGCGTCGAACTGAAGCTGTCGTTCGACGGCCCGCCACCCCAGCCCAAGG
GCTACACGACCGAGTCGCCCGCGCGTATTGCACTGGATCTGCCGGGGGTGGCCAGCCAGTTGGCCAACAGAAATCTTGAT
CTGGGCAGCGGCAATGCGCGCACGGCCACGGTGGCCGAAGCCAAGGATCGCACGCGGCTGATTGTCAGCCTGACGCAATT
GGCGCCATACAGCACGCGGGTCGAAGGCAATAATCTGTTCGTGGTGGTTGGTCAGGGCGCCTCCGCGACCGCGCCGCGCC
CGGCCGCCGTTGCACCACGTGCCGCGACGGCGACTTCGGCGCCCGCCAGATCCGCGGCGCCGCGCAGCCGGTCGATTCGC
GGCGTTGATTTCCAGCGCGGCACCGAAGGCGAGGGCAACGTGGTCATCGACCTCTCTGACCCGACCATCGCCCCGGATAT
CCAGGAGCACGACGGCAAGATCATCCTCAGCTTCGCCCGCACCCAATTGCCGGAAAAACTGCGCGTGCGCCTCGACGTCA
AAGACTTCGCCACACCGGTGCAGTTCGTCAACGCAGCGCTCACCGGCGATCGCACCGTGATCAGTGTCGAGCCCAGCGGC
ACCTTCGACTATTCGACCTTCCAGACTGATAACAAGCTCACGGTGAGCGTTCGCCCGATGAGTGTCGACGATCTGCAAAA
GCGCAACGCCGACCGTCAGGCCTATGTCGGCGACAAGCTGTCGCTGAATTTTCAGGACATCGATGTACGCTCGGTGCTGC
AACTGATCGCAGACTTCACCAATCTCAACCTCGTGGCAAGCGACACGGTGCAGGGCGGCATCACGTTGCGCCTGCAGAAC
GTGCCGTGGGATCAGGCACTGGATCTGGTGTTGAAAACCAAAGGTCTGGATAAACGCAAGATCGGCAACGTGCTACTGGT
GGCGCCGGCCGATGAAATTGCCGCCCGCGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATCGCCGAGCTGGCGCCATTGC
GTCGCGAGCTGCTGCAAGTCAATTACGCCAAGGCTGCGGACATTGCCAAACTGTTCCAGTCGGTGACCAGTGCCGAGGCC
AAGGTCGACGAGCGCGGTTCGATCACTGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
CGAATTACGGCGGATCGTCGCGCAGCTGGATATTCCGGTACGTCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCAACG
TCGATTACGACAAGAGCCTCGGCGTACGCTGGGGCGGCTCGGTGCAGAACAAGGGCAACTGGAACGCCTCCGGGGTCAAC
GGCTCATCGAGCACCATCGGTACGCCGGGCAGCACCAGCACCAACTCGCCCTTCGTCGACATGGGCACGGTCAACAATAC
CTCCGGGATCGGTATCGCGTTCATCACCGACAACGTGTTGCTGGACCTGGAACTGACGGCGATGGAAAAGACCGGCAACG
GCGAGATCGTCTCGCAGCCGAAGGTGGTCACCTCGGACAAGGAAACCGCGAAGATCCTCAAAGGCACCGAGATACCGTAT
CAGGAGGCCAGCTCCAGTGGCGCGACCTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAAGTCACTCCGCAGATCAC
GCCTGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCCGACTACCTGAACAAAGTGCAGGATGTACCGC
CGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGGTGAATGACGGCGAGACCATCGTAATCGGCGGTGTTTTTTCAAAT
ACTCAAAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(37-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Bacterial type II and III secretion system protein (Secretin)

(527-683)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.067

100

0.746
  comQ Acinetobacter baylyi ADP1

41.474

100

0.415
  pilQ Acinetobacter baumannii strain A118

38.306

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.306

100

0.412
  pilQ Legionella pneumophila strain Lp02

37.85

98.121

0.371
  pilQ Legionella pneumophila strain ERS1305867

37.814

97.832

0.37