Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MKFW12EY_RS00740 Genome accession   NZ_AP019777
Coordinates   151318..152472 (-) Length   384 a.a.
NCBI ID   WP_054759524.1    Uniprot ID   A0A172U3G9
Organism   Methylomonas koyamae strain Fw12E-Y     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 146318..157472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKFW12EY_RS00715 (MKFW12EY_01400) - 146842..147564 (+) 723 WP_054759463.1 cytochrome c1 -
  MKFW12EY_RS00720 (MKFW12EY_01410) ftsH 147651..149564 (-) 1914 WP_082409670.1 ATP-dependent zinc metalloprotease FtsH -
  MKFW12EY_RS00725 (MKFW12EY_01420) lexA 150135..150764 (+) 630 WP_054759465.1 transcriptional repressor LexA -
  MKFW12EY_RS00730 (MKFW12EY_01430) - 150777..150926 (+) 150 WP_157198033.1 hypothetical protein -
  MKFW12EY_RS00735 (MKFW12EY_01440) - 150972..151295 (+) 324 WP_054759467.1 hypothetical protein -
  MKFW12EY_RS00740 (MKFW12EY_01450) pilU 151318..152472 (-) 1155 WP_054759524.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MKFW12EY_RS00745 (MKFW12EY_01460) - 152634..155243 (+) 2610 WP_221053828.1 PAS domain-containing protein -
  MKFW12EY_RS00750 (MKFW12EY_01470) - 155240..155872 (+) 633 WP_096875527.1 response regulator transcription factor -
  MKFW12EY_RS00755 (MKFW12EY_01480) - 156040..157083 (+) 1044 WP_064020088.1 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase -
  MKFW12EY_RS00760 (MKFW12EY_01490) - 157080..157304 (+) 225 WP_054759473.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 384 a.a.        Molecular weight: 42483.63 Da        Isoelectric Point: 6.0981

>NTDB_id=73938 MKFW12EY_RS00740 WP_054759524.1 151318..152472(-) (pilU) [Methylomonas koyamae strain Fw12E-Y]
MEFRDYLKILVQHDGSDLYLTAGAPPAAKFQGTLKPLENVKLTPERIRQIADSVMDNEQRASFEHVPEMNLAITEEGIGR
FRVNIFKQRNCYALVIRNIKVDIPNADVLGLPQILKDKIMEKRGLILFVGGTGSGKSTSLAALIDYRNSNSSGHIITIED
PIEFVHPHKRSLVNQREVGVDTLSYEDALKNTLRQAPDVILIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRPQLLMDLSLNLQAFVSQRLVPTIEGKRVAAIEILLGTKLVSDLIHKGDIHAIKEAMEKSENVGMQTFDSHL
LKLYKSGVISLEEAMRNSDSPNNLKLKINLSEGLGSATKPAGALGSLALEEISKKEGEDGDEGH

Nucleotide


Download         Length: 1155 bp        

>NTDB_id=73938 MKFW12EY_RS00740 WP_054759524.1 151318..152472(-) (pilU) [Methylomonas koyamae strain Fw12E-Y]
ATGGAATTTAGAGACTATTTGAAAATCCTGGTGCAGCACGACGGTTCCGATTTGTATTTGACGGCCGGTGCGCCGCCGGC
TGCAAAGTTTCAAGGCACCTTGAAGCCGTTGGAAAACGTCAAGCTGACTCCGGAACGGATCAGACAGATTGCCGACAGCG
TGATGGACAACGAGCAGCGCGCCAGTTTCGAGCACGTACCGGAGATGAATCTGGCGATTACCGAAGAGGGCATCGGCCGA
TTCCGGGTCAATATTTTCAAGCAACGCAATTGTTATGCGTTGGTGATCCGCAATATCAAGGTGGATATTCCGAATGCCGA
CGTTTTAGGCTTGCCGCAAATTTTGAAAGACAAAATCATGGAAAAGCGCGGCCTGATTTTGTTCGTCGGCGGCACCGGTT
CCGGCAAATCGACCTCGCTGGCCGCCTTGATCGATTACCGCAACAGCAATTCGTCGGGCCACATCATCACCATCGAAGAC
CCGATCGAATTCGTTCACCCGCATAAGCGTTCGCTGGTCAACCAGCGCGAAGTCGGCGTCGACACGCTGAGTTACGAGGA
CGCATTGAAAAACACCTTACGCCAGGCGCCCGACGTGATCCTGATCGGCGAAATCCGCAGCCAGGAAACGATGGAACATG
CGTTAGCCTTTGCCGAAACCGGACATTTGTGCCTGTCCACCTTGCATGCCAATAACGCCAACCAGGCGCTGGATAGGATC
ATCAATTTCTTCCCGGAAGAGCGCCGGCCGCAATTGTTGATGGACTTGTCGTTGAATTTGCAGGCCTTCGTTTCGCAACG
CCTGGTACCGACCATCGAAGGCAAGCGGGTTGCGGCAATCGAAATCCTGTTGGGCACTAAATTGGTCAGCGATTTGATCC
ATAAGGGCGACATTCACGCGATCAAGGAGGCGATGGAAAAGTCGGAAAACGTCGGCATGCAGACCTTCGACAGCCATCTA
TTGAAGCTGTACAAAAGCGGTGTGATCTCGCTGGAAGAAGCGATGCGCAATTCCGATTCGCCGAACAATCTGAAATTGAA
AATCAACTTGAGCGAGGGTTTGGGTTCGGCGACCAAACCGGCCGGGGCGCTGGGCAGTCTGGCTTTGGAAGAAATCAGCA
AGAAGGAAGGCGAGGACGGCGACGAAGGCCATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(79-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172U3G9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.12

91.406

0.531
  pilU Acinetobacter baylyi ADP1

54.696

94.271

0.516
  pilU Vibrio cholerae strain A1552

49.435

92.188

0.456
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.052

100

0.404
  pilT Legionella pneumophila strain Lp02

42.424

85.938

0.365
  pilT Legionella pneumophila strain ERS1305867

42.424

85.938

0.365

Multiple sequence alignment