Detailed information NTG0739346

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OFU58_RS14770
• Genome accession: NZ_CP107289
• Coordinates: 3037923..3039041 (+)
• Length: 372 a.a.
• NCBI ID: WP_060465360.1
• Uniprot ID: A0A2I6LWN2
• Organism: Acinetobacter pittii strain BM4623
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3032923..3044041

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OFU58_RS14760 (OFU58_14765)	yggS	3036047..3036739 (-)	693	WP_057085996.1	YggS family pyridoxal phosphate-dependent enzyme	-
OFU58_RS14765 (OFU58_14770)	pilT	3036866..3037903 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
OFU58_RS14770 (OFU58_14775)	pilU	3037923..3039041 (+)	1119	WP_060465360.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OFU58_RS14775 (OFU58_14780)	fur	3039111..3039548 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
OFU58_RS14780 (OFU58_14785)	omlA	3039660..3040058 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
OFU58_RS14785 (OFU58_14790)	-	3040123..3040437 (-)	315	WP_280585653.1	RnfH family protein	-
OFU58_RS14790 (OFU58_14795)	-	3040437..3041504 (-)	1068	WP_031944146.1	membrane protein	-
OFU58_RS14795 (OFU58_14800)	-	3041658..3042113 (-)	456	WP_002121698.1	bacteriohemerythrin	-
OFU58_RS14800 (OFU58_14805)	-	3042365..3043213 (-)	849	WP_280588827.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41917.12 Da
• Isoelectric Point: 6.6403

>NTDB_id=739346 OFU58_RS14770 WP_060465360.1 3037923..3039041(+) (pilU) [Acinetobacter pittii strain BM4623]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=739346 OFU58_RS14770 WP_060465360.1 3037923..3039041(+) (pilU) [Acinetobacter pittii strain BM4623]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGACAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAGACTCGTGAATGTAATTTTGCCATTATGAACCGCGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAACAGCGTGACATGCCCGGCATGGTACTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCACATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACATGCTGGTTGCATCATTACCCAACGTGAAGTCGGGATTGATACAGACTCATTCGA
AATTGCCTTAAAGAATACTTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATTCGCTCTCGTGAAGTCATGGACT
ATGCGATTGGTTTTGCCGAAACAGGTCACCTTGTATTAGCCACAATGCATGCGAACAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCTCGCCGTGCAGCGATTGAGATTCTCATTAACTCACCATTAATTTCAGACT
ATATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCGGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTGCG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (33-270)

3D structure

• AlphaFold DB: A0A2I6LWN2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.397	100	0.766
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363