Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   QAY87_RS22060 Genome accession   NZ_CP121766
Coordinates   4657482..4658891 (-) Length   469 a.a.
NCBI ID   WP_023108391.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 22112     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4652482..4663891
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QAY87_RS22050 (QAY87_22045) - 4654576..4655754 (-) 1179 WP_023434839.1 substrate-binding domain-containing protein -
  QAY87_RS22055 (QAY87_22050) pilC 4656268..4657482 (-) 1215 WP_003112721.1 type II secretion system inner membrane protein GspF Machinery gene
  QAY87_RS22060 (QAY87_22055) pilF 4657482..4658891 (-) 1410 WP_023108391.1 type II secretion system ATPase GspE Machinery gene
  QAY87_RS22065 (QAY87_22060) gspD 4658888..4661299 (-) 2412 WP_014603262.1 type II secretion system secretin GspD -
  QAY87_RS22070 (QAY87_22065) - 4661296..4661889 (-) 594 WP_003112724.1 type II secretion system protein M -
  QAY87_RS22075 (QAY87_22070) gspL 4661876..4663087 (-) 1212 WP_058201607.1 type II secretion system protein GspL -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 51021.43 Da        Isoelectric Point: 5.7943

>NTDB_id=738677 QAY87_RS22060 WP_023108391.1 4657482..4658891(-) (pilF) [Pseudomonas aeruginosa strain 22112]
MSLLPYAWAKAQRALLRPGEHGATLLVSPRTPGWAISEVRQRHALASLESVRDDELDTLLASAYSDTGSAAAVVGAAENE
VDLDRLMDDIPEVTDLLDTQDGAPVIRMINALLTQAARDEASDIHIEPFETHSVVRYRVDGALRDVVAPRKALHAALVSR
IKIMAQLDIAEKRLPQDGRIALRVAGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLRLETLGMAPGVLAPLDNLIRQPHG
IVLVTGPTGSGKTTTLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNARIDMTFAVALRAILRQDPDIIMIGEIRDL
ETAQIAVQASLTGHLVLATLHTNDAVSAVTRLVDMGVEPFLLASSMLGVLAQRLVRRLCTHCRVEEDGGWRAVGCPACNQ
TGYSGRTGIHELFVIDDEIRRLVHQGRAEQDLREAARAAGMRSMREDGERWIASGSTTLEEILRVTRDA

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=738677 QAY87_RS22060 WP_023108391.1 4657482..4658891(-) (pilF) [Pseudomonas aeruginosa strain 22112]
ATGAGCCTGCTGCCCTACGCCTGGGCCAAGGCGCAGCGCGCCTTGCTGCGGCCGGGCGAGCATGGCGCGACCCTGCTGGT
CAGCCCGCGCACGCCCGGCTGGGCGATCAGCGAGGTGCGCCAGCGGCATGCGCTGGCCAGCCTGGAGAGCGTCCGCGACG
ACGAACTCGACACCCTGCTGGCCAGCGCCTACTCCGATACCGGCAGCGCCGCGGCGGTGGTCGGCGCGGCGGAGAACGAG
GTCGACCTCGACCGCCTGATGGACGATATCCCCGAGGTCACCGATCTGCTCGATACCCAGGATGGCGCGCCGGTGATCCG
CATGATCAACGCGCTGCTGACCCAGGCGGCGCGCGACGAGGCCAGCGACATCCACATCGAGCCCTTCGAGACCCATTCGG
TGGTGCGCTACCGCGTCGACGGCGCCCTGCGCGACGTGGTCGCGCCGCGCAAGGCGCTGCACGCGGCGCTGGTGTCGCGG
ATCAAGATCATGGCGCAGCTGGATATCGCCGAGAAACGCCTGCCCCAGGATGGCCGCATCGCCTTGCGGGTAGCCGGGCG
GCCGATCGACATTCGCGTCTCCACCGTGCCCACCGGCCACGGCGAACGGGTGGTGATGCGCCTGCTCGACAAGCAGGCCG
GGCGCCTGAGGCTGGAGACCCTGGGCATGGCTCCCGGCGTGCTGGCGCCGCTGGACAACCTGATCCGCCAGCCGCACGGC
ATCGTGCTGGTCACCGGGCCCACCGGCAGCGGCAAGACCACTACCCTCTACGCCGCCCTGGCGCGGCTCGATGCCAGCAC
CAGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGACCTGCCGGGGATCAGCCAGATCCAGGTCAACGCGCGCATCG
ACATGACCTTCGCCGTAGCCCTGCGGGCGATCCTCCGGCAGGACCCGGACATCATCATGATCGGCGAGATCCGCGACCTG
GAGACCGCGCAGATCGCCGTGCAGGCCTCGCTCACCGGGCACCTGGTGCTGGCCACGCTGCACACCAACGACGCGGTGTC
GGCGGTGACCCGGCTGGTGGACATGGGCGTCGAGCCGTTCCTGCTCGCCTCGTCGATGCTTGGCGTACTCGCCCAGCGCC
TGGTGCGGCGCCTGTGCACGCATTGCCGGGTCGAAGAGGACGGCGGCTGGCGCGCGGTCGGCTGCCCGGCCTGCAACCAG
ACCGGCTACAGCGGGCGCACCGGCATCCACGAACTGTTCGTGATCGACGACGAGATCCGCCGCCTGGTCCACCAGGGCCG
CGCCGAGCAGGATCTCCGCGAAGCCGCCCGCGCCGCCGGCATGCGCAGCATGCGCGAGGACGGCGAGCGCTGGATCGCCA
GCGGCTCCACCACCCTGGAGGAAATCCTCCGCGTGACCCGGGACGCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(111-377)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

50.928

80.384

0.409
  pilB/pilB1 Synechocystis sp. PCC 6803

41.113

99.574

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.462

83.156

0.403
  pilB Vibrio cholerae strain A1552

46.97

84.435

0.397
  pilB Legionella pneumophila strain ERS1305867

43.256

91.684

0.397
  pilF Neisseria gonorrhoeae MS11

47.208

84.009

0.397
  pilB Acinetobacter baumannii D1279779

41.403

94.243

0.39
  pilB Vibrio campbellii strain DS40M4

46.329

84.222

0.39
  pilB Vibrio parahaemolyticus RIMD 2210633

46.076

84.222

0.388
  pilB Acinetobacter baylyi ADP1

45.103

82.729

0.373