Detailed information NTG0737584

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: ODS73_RS10690
• Genome accession: NZ_CP107038
• Coordinates: 2040954..2041415 (-)
• Length: 153 a.a.
• NCBI ID: WP_000250523.1
• Uniprot ID: A0A0B7M7T3
• Organism: Streptococcus pneumoniae strain BM6001
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2035954..2046415

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ODS73_RS10655 (ODS73_10645)	-	2036258..2037082 (-)	825	WP_000727904.1	membrane protein insertase YidC	-
ODS73_RS10660 (ODS73_10650)	rnpA	2037057..2037428 (-)	372	WP_000739246.1	ribonuclease P protein component	-
ODS73_RS10665 (ODS73_10655)	-	2037445..2037576 (-)	132	WP_000768904.1	hypothetical protein	-
ODS73_RS10670 (ODS73_10660)	-	2037577..2038767 (-)	1191	WP_284060558.1	acetate kinase	-
ODS73_RS10675 (ODS73_10665)	comYH	2038818..2039771 (-)	954	WP_221015135.1	class I SAM-dependent methyltransferase	Machinery gene
ODS73_RS10680 (ODS73_10670)	-	2039832..2040426 (-)	595	Protein_2091	class I SAM-dependent methyltransferase	-
ODS73_RS10685 (ODS73_10675)	comGG/cglG	2040563..2040976 (-)	414	WP_284060559.1	competence type IV pilus minor pilin ComGG	Machinery gene
ODS73_RS10690 (ODS73_10680)	comGF/cglF	2040954..2041415 (-)	462	WP_000250523.1	competence type IV pilus minor pilin ComGF	Machinery gene
ODS73_RS10695 (ODS73_10685)	comGE/cglE	2041378..2041680 (-)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
ODS73_RS10700 (ODS73_10690)	comGD/cglD	2041643..2042047 (-)	405	WP_000588005.1	competence type IV pilus minor pilin ComGD	Machinery gene
ODS73_RS10705 (ODS73_10695)	comGC/cglC	2042040..2042306 (-)	267	WP_000962026.1	competence type IV pilus major pilin ComGC	Machinery gene
ODS73_RS10710 (ODS73_10700)	-	2042308..2042565 (-)	258	WP_050266402.1	hypothetical protein	-
ODS73_RS10715 (ODS73_10705)	-	2042795..2043751 (-)	957	WP_284060560.1	N-acetylmuramoyl-L-alanine amidase family protein	-
ODS73_RS10720 (ODS73_10710)	-	2043755..2044087 (-)	333	WP_284060561.1	phage holin	-
ODS73_RS10725 (ODS73_10715)	-	2044091..2044507 (-)	417	WP_000687454.1	phage holin family protein	-
ODS73_RS10730 (ODS73_10720)	-	2044517..2044780 (-)	264	WP_050266966.1	hypothetical protein	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17821.32 Da
• Isoelectric Point: 6.4832

>NTDB_id=737584 ODS73_RS10690 WP_000250523.1 2040954..2041415(-) (comGF/cglF) [Streptococcus pneumoniae strain BM6001]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide

• Length: 462 bp

>NTDB_id=737584 ODS73_RS10690 WP_000250523.1 2040954..2041415(-) (comGF/cglF) [Streptococcus pneumoniae strain BM6001]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

3D structure

• AlphaFold DB: A0A0B7M7T3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae D39	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae R6	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae TIGR4	94.771	100	0.948
comGF/cglF	Streptococcus mitis SK321	93.464	100	0.935
comGF/cglF	Streptococcus mitis NCTC 12261	92.157	100	0.922