Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N7L95_RS10265 Genome accession   NZ_CP106951
Coordinates   2349545..2351263 (+) Length   572 a.a.
NCBI ID   WP_301259723.1    Uniprot ID   -
Organism   Eleftheria terrae strain P9846-PB     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2344545..2356263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7L95_RS10235 (N7L95_10250) proB 2344775..2345893 (-) 1119 WP_301259719.1 glutamate 5-kinase -
  N7L95_RS10240 (N7L95_10255) obgE 2345935..2347041 (-) 1107 WP_301259720.1 GTPase ObgE -
  N7L95_RS10245 (N7L95_10260) rpmA 2347319..2347576 (-) 258 WP_301259721.1 50S ribosomal protein L27 -
  N7L95_RS10250 (N7L95_10265) rplU 2347611..2347922 (-) 312 WP_301259722.1 50S ribosomal protein L21 -
  N7L95_RS10255 (N7L95_10270) ispB 2348238..2349167 (+) 930 WP_301260118.1 octaprenyl diphosphate synthase -
  N7L95_RS10265 (N7L95_10280) pilB 2349545..2351263 (+) 1719 WP_301259723.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N7L95_RS10270 (N7L95_10285) pilC 2351307..2352527 (+) 1221 WP_301259724.1 type II secretion system F family protein Machinery gene
  N7L95_RS10275 (N7L95_10290) - 2352543..2353397 (+) 855 WP_301259725.1 A24 family peptidase -
  N7L95_RS10280 (N7L95_10295) coaE 2353434..2354039 (+) 606 WP_301259726.1 dephospho-CoA kinase -
  N7L95_RS10285 (N7L95_10300) zapD 2354188..2354943 (+) 756 WP_301259727.1 cell division protein ZapD -
  N7L95_RS10290 (N7L95_10305) yacG 2354943..2355164 (+) 222 WP_301259728.1 DNA gyrase inhibitor YacG -
  N7L95_RS10295 (N7L95_10310) - 2355198..2355641 (-) 444 WP_301259730.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62249.49 Da        Isoelectric Point: 5.8830

>NTDB_id=736790 N7L95_RS10265 WP_301259723.1 2349545..2351263(+) (pilB) [Eleftheria terrae strain P9846-PB]
MDTLAEAPNLTLSGVARVLVNAGKLNAKTAEDLAKAAKEKNTSFIAAVIAAGAVSSTDLAHALSTAFSLPLIDLSAVDAT
KLPRNLIEAKLAAQYQVAVLGKRGSRLFVAGADPTNQEALERIKFATQLTPEWVVVEHDKLVKLLESSGANASEALDNIV
GGEFDFEVTDEESTPQESQEVTTDVEDAPVVRFLQKMLIDAINLRASDLHFEPYEQNYRVRFRVDGELREITQPPIAIKE
KLASRIKVISRLDIAEKRVPQDGRMKLKFGNRAIDFRVSTLPTLFGEKIVIRILDPSSAKLGIEALGYEKEEKDRLLKAI
ERPYGMVLVTGPTGSGKTVSLYTCLNMLNKPGVNISTVEDPAEINLPGINQVNVNDKAGLNFSTALKAFLRQDPDVIMVG
EIRDLETADIAIKAAQTGHMVMSTLHTNDAPTTLTRLRNMGVAPFNIASSVILITAQRLARRLCESCKSPATYPKEALLR
AGFKDSDLDGTWKPYRAVGCSNCNNGYKGRVGLYQVMPISEEIQRIILADGSAMDIAAQAQKEGVRDLRQAGLLKVKLGV
TTLEEVISVTNE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=736790 N7L95_RS10265 WP_301259723.1 2349545..2351263(+) (pilB) [Eleftheria terrae strain P9846-PB]
ATGGACACACTGGCTGAGGCTCCCAACCTGACCCTGTCTGGCGTTGCGCGAGTGCTTGTGAATGCGGGCAAGCTCAACGC
CAAGACCGCGGAAGACCTGGCGAAGGCCGCCAAGGAAAAGAACACGAGCTTCATCGCGGCCGTCATCGCGGCCGGCGCTG
TTTCTTCGACCGACCTCGCTCATGCGCTGTCGACCGCCTTCTCCCTGCCCCTGATCGACCTGTCGGCGGTCGATGCCACC
AAGCTGCCACGCAACCTCATCGAGGCGAAGTTGGCTGCACAGTACCAGGTGGCGGTGCTCGGCAAGCGCGGCAGCCGGCT
GTTTGTCGCCGGCGCCGACCCGACCAACCAGGAAGCGCTGGAGCGCATCAAGTTCGCGACGCAGCTGACACCCGAGTGGG
TGGTCGTCGAGCACGACAAGCTGGTCAAGCTGCTGGAGAGCAGTGGCGCCAATGCGAGCGAGGCACTCGACAACATCGTC
GGTGGCGAGTTCGACTTCGAGGTCACGGACGAAGAAAGCACGCCCCAGGAATCGCAAGAGGTCACCACCGATGTGGAGGA
TGCGCCGGTCGTGCGCTTCCTGCAGAAGATGCTGATCGACGCCATCAACCTGCGCGCCTCCGACCTGCATTTCGAGCCCT
ACGAGCAGAACTACCGCGTGCGCTTTCGCGTCGACGGCGAGTTGCGTGAGATCACCCAGCCGCCGATCGCCATCAAGGAA
AAGCTGGCGTCCCGCATCAAGGTCATCTCCCGGCTCGACATTGCCGAAAAACGTGTGCCGCAAGACGGTCGCATGAAGCT
GAAGTTCGGCAACCGGGCCATCGACTTCCGGGTCAGCACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATCCGGATTC
TCGACCCCTCCAGTGCGAAGCTCGGCATCGAGGCCCTGGGCTATGAGAAGGAAGAGAAGGACCGGCTGCTGAAGGCCATT
GAGCGTCCCTACGGTATGGTCCTGGTGACCGGCCCGACCGGGAGTGGCAAAACCGTGTCGCTGTACACCTGCCTCAACAT
GCTCAACAAGCCGGGCGTGAACATCTCGACCGTGGAGGACCCGGCCGAAATCAACCTGCCGGGCATCAACCAGGTCAATG
TGAATGACAAGGCCGGCCTCAACTTCTCGACCGCACTGAAGGCCTTCCTCCGCCAGGATCCGGACGTCATCATGGTCGGC
GAAATTCGCGACCTCGAGACGGCTGACATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGATGTCGACGTTGCATAC
GAACGACGCACCGACGACTTTGACTCGTTTGCGCAACATGGGGGTCGCCCCATTCAATATCGCGTCGAGCGTGATATTGA
TCACAGCACAGCGCCTGGCGCGCCGACTGTGTGAAAGTTGCAAATCGCCGGCGACCTATCCGAAAGAAGCCTTGCTGCGT
GCGGGATTCAAGGATTCGGACCTCGACGGAACGTGGAAACCTTATCGCGCAGTTGGCTGCAGTAATTGCAACAATGGGTA
TAAAGGTCGTGTCGGCCTTTACCAAGTGATGCCGATCTCCGAAGAGATTCAGCGCATCATCCTGGCCGACGGGAGCGCGA
TGGACATTGCCGCACAAGCCCAGAAAGAGGGAGTGCGCGATCTCCGCCAGGCAGGGCTGCTGAAGGTCAAACTGGGCGTC
ACCACGCTGGAAGAAGTGATCTCGGTCACGAACGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.704

100

0.549
  pilF Neisseria gonorrhoeae MS11

53.415

99.825

0.533
  pilB Acinetobacter baylyi ADP1

53.819

98.427

0.53
  pilB Legionella pneumophila strain ERS1305867

50.265

98.776

0.496
  pilB Vibrio cholerae strain A1552

46.004

98.427

0.453
  pilB Vibrio parahaemolyticus RIMD 2210633

45.439

99.65

0.453
  pilB Vibrio campbellii strain DS40M4

45.714

97.902

0.448
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.866

92.832

0.379
  pilF Thermus thermophilus HB27

36.972

99.301

0.367