Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   N9L84_RS27200 Genome accession   NZ_CP106785
Coordinates   5950100..5951164 (-) Length   354 a.a.
NCBI ID   WP_173171164.1    Uniprot ID   -
Organism   Pseudomonas tohonis strain G5.110     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5945100..5956164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N9L84_RS27175 (N9L84_27175) aroK 5945724..5946240 (-) 517 Protein_5380 shikimate kinase AroK -
  N9L84_RS27180 (N9L84_27180) pilQ 5946246..5948324 (-) 2079 WP_263151514.1 type IV pilus secretin PilQ Machinery gene
  N9L84_RS27185 (N9L84_27185) pilP 5948378..5948911 (-) 534 WP_173171170.1 type 4a pilus biogenesis lipoprotein PilP -
  N9L84_RS27190 (N9L84_27190) pilO 5948908..5949531 (-) 624 WP_173171168.1 type 4a pilus biogenesis protein PilO -
  N9L84_RS27195 (N9L84_27195) - 5949528..5950100 (-) 573 WP_173171166.1 PilN domain-containing protein -
  N9L84_RS27200 (N9L84_27200) pilM 5950100..5951164 (-) 1065 WP_173171164.1 pilus assembly protein PilM Machinery gene
  N9L84_RS27205 (N9L84_27205) - 5951349..5953769 (+) 2421 WP_263154508.1 penicillin-binding protein 1A -
  N9L84_RS27210 (N9L84_27210) - 5953883..5955151 (-) 1269 WP_173171162.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37680.91 Da        Isoelectric Point: 4.4429

>NTDB_id=735126 N9L84_RS27200 WP_173171164.1 5950100..5951164(-) (pilM) [Pseudomonas tohonis strain G5.110]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLGKAKASSKSVAV
AVAGSAVITKTIEMDAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGASARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLAAQLGNGSDELTVAVVDIGATMTTLSVLHNGRTIYSREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTQVANP
FAEMALSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=735126 N9L84_RS27200 WP_173171164.1 5950100..5951164(-) (pilM) [Pseudomonas tohonis strain G5.110]
GTGCTAGGGCTCTTCAACAAGAAAGCGAATACGCTGCTGGGGATCGACATAAGCTCGACTTCAGTCAAGCTCCTTGAATT
GAGTCGCTCGGGAAGCCGCTACAAGGTAGAGGCCTACGCCGTCGAGCCGCTCCCACCGAATGCTGTGGTCGAAAAGAACA
TCGCCGAACTGGAGGGGGTCGGCCAGGCGTTGTCTCGTGTTCTTGGCAAGGCCAAGGCCAGTTCGAAGTCCGTCGCCGTA
GCGGTCGCGGGCTCCGCTGTAATCACCAAGACGATCGAGATGGATGCCGGTCTTTCCGACGATGAGTTGGAGAACCAGCT
GAAGATCGAGGCGGACCAGTACATCCCTTACCCGCTCGAAGAAGTCGCGATCGACTTCGAGGTCCAGGGTGCTTCCGCGC
GCAACCCTGAGCGGGTCGATGTGCTCCTCGCCGCGTGCCGCAAGGAGAACGTCGAGGTCCGTGAAGCCGCTCTGGCCCTG
GCGGGCCTGACCGCCAAGGTCGTCGACGTCGAAGCCTATGCGCTGGAGCGTGCCTACGGACTTCTCGCTGCGCAACTGGG
CAACGGGAGTGACGAACTGACGGTCGCCGTCGTGGATATCGGGGCGACCATGACGACCCTGAGCGTCCTGCACAACGGCC
GGACCATCTACAGCCGCGAGCAGCTTTTCGGCGGGCGCCAGCTCACCGAGGAGATCCAGCGCCGCTATGGCCTGTCCGTG
GAAGAAGCGGGTCTTGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTGTTGCAACCGTTCAAGGACGC
AGTGGTCCAGCAAGTGTCTCGTTCGCTGCAGTTCTTCTTCGCTGCGGGCCAGTTCAACGATGTCGACTACATCCTCCTGG
CGGGTGGCACGGCGTCCATTCCTGATCTGGATCGTTTGATCCAGCAGAAGATCGGCACTCCTACCCAGGTTGCCAACCCC
TTCGCGGAGATGGCGCTGAGTGGCAAGGTCAATGCGGGCGCATTGGCCAGTGATGCCCCGGCGCTGATGATTGCCTGCGG
ACTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

57.062

100

0.571
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  comM Acinetobacter baylyi ADP1

53.39

100

0.534
  pilM Legionella pneumophila strain ERS1305867

47.74

100

0.477