Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OEG80_RS12360 Genome accession   NZ_CP106752
Coordinates   2914814..2916526 (+) Length   570 a.a.
NCBI ID   WP_263241071.1    Uniprot ID   A0A9E8XAN0
Organism   Psychrobacter sp. SC65A.3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2909814..2921526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OEG80_RS12330 (SC65A3_02486) nusA 2910048..2911532 (-) 1485 WP_011512448.1 transcription termination factor NusA -
  OEG80_RS12335 (SC65A3_02487) rimP 2911666..2912166 (-) 501 WP_011512447.1 ribosome maturation factor RimP -
  OEG80_RS12350 (SC65A3_02490) secG 2913070..2913378 (-) 309 WP_011512446.1 preprotein translocase subunit SecG -
  OEG80_RS12355 (SC65A3_02491) tpiA 2913607..2914416 (-) 810 WP_263241070.1 triose-phosphate isomerase -
  OEG80_RS12360 (SC65A3_02492) pilB 2914814..2916526 (+) 1713 WP_263241071.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OEG80_RS12365 (SC65A3_02493) pilC 2916754..2917977 (+) 1224 WP_181209343.1 type II secretion system F family protein Machinery gene
  OEG80_RS12370 (SC65A3_02494) pilD 2918003..2918902 (+) 900 WP_263241073.1 A24 family peptidase Machinery gene
  OEG80_RS12375 (SC65A3_02495) coaE 2918986..2919678 (+) 693 WP_263241074.1 dephospho-CoA kinase -
  OEG80_RS12380 (SC65A3_02496) rlmB 2919641..2920411 (-) 771 WP_133103981.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  OEG80_RS12385 (SC65A3_02497) - 2920616..2920951 (-) 336 WP_020442457.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63160.51 Da        Isoelectric Point: 5.3322

>NTDB_id=734454 OEG80_RS12360 WP_263241071.1 2914814..2916526(+) (pilB) [Psychrobacter sp. SC65A.3]
MSITTSKYGGLAQQLISEGIVSEAHMKAAQTDSQQQQVGLVSYLVEHKLANAYQLAQMLSQAFGDPLFDLDTLSSEVIPK
DLVDEKIVRKFNALPMFKRGQRLFVALSDPTRIDAIDAIAFNSRLSIETVVVEEDKLKRRIESFYADSMQNFDSFSNSDL
HIGFDESQEEDNGETKISDSVEEAPVVKFVNKMLVDAIRMGASDLHFEPYEKSYRVRFRVDGVMQKMANPPVQLAGKIAA
RLKVMSQMDISERRVPQDGRIKLKISKDKAIDFRVSCLPTLFGEKLVLRILDPSSAMLGIEALGYEPDQQDMFLEALHKP
QGMLLITGPTGSGKTVSLYTGINILNTGSTNISTAEDPVEINLEGINQVNVNPKVGLTFSTALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKKPISIPRQSLLELGF
TDADLDNTDNIIYEPVGCNECREGYKGRVGIYEVMKVTPDISRIIMEDGNAIDIKDAALKNGFRDLRRSGVLKVLQGVTS
IQEMMRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=734454 OEG80_RS12360 WP_263241071.1 2914814..2916526(+) (pilB) [Psychrobacter sp. SC65A.3]
ATGTCTATCACAACCAGTAAATACGGTGGCTTAGCACAGCAACTGATTAGCGAAGGTATCGTAAGTGAAGCGCATATGAA
AGCGGCGCAGACTGATTCGCAGCAGCAGCAAGTAGGACTGGTCTCTTATCTGGTAGAGCATAAGCTCGCCAATGCCTATC
AATTGGCTCAAATGCTATCGCAAGCATTCGGCGATCCTTTATTTGATCTTGATACGCTCAGCTCAGAGGTCATCCCAAAA
GACTTGGTCGACGAAAAAATCGTCCGTAAGTTTAATGCCTTGCCAATGTTTAAGCGTGGGCAACGCTTATTTGTGGCACT
GAGTGATCCGACCCGTATCGATGCGATTGATGCTATTGCCTTTAACTCTCGCTTGTCGATTGAAACAGTCGTGGTCGAAG
AAGATAAGCTTAAAAGGCGTATTGAAAGCTTCTATGCTGATAGCATGCAGAATTTTGATAGCTTTTCTAATAGTGATTTG
CATATTGGCTTTGATGAGAGTCAAGAAGAAGATAACGGCGAGACCAAAATCTCTGATAGCGTCGAGGAAGCACCGGTCGT
CAAGTTTGTTAATAAAATGCTAGTAGATGCCATTCGTATGGGTGCGTCCGACTTACATTTTGAGCCCTATGAAAAAAGCT
ATCGAGTGCGCTTTCGAGTTGATGGGGTCATGCAAAAAATGGCCAACCCGCCCGTACAACTCGCGGGGAAAATTGCTGCG
CGTTTAAAAGTGATGTCACAAATGGATATCTCAGAGCGCCGGGTGCCACAAGATGGTCGTATTAAGCTTAAGATATCTAA
AGATAAGGCCATCGACTTTCGGGTAAGCTGCCTACCTACCTTGTTTGGTGAAAAGCTGGTTTTGCGTATTCTAGATCCCT
CATCAGCCATGCTTGGTATTGAAGCGTTGGGCTATGAGCCTGACCAACAAGATATGTTCTTGGAGGCGTTACATAAACCG
CAAGGGATGCTTCTTATCACAGGTCCTACTGGCTCTGGTAAAACCGTCTCACTTTATACTGGTATTAATATCTTGAACAC
GGGTTCGACCAATATTTCGACTGCTGAAGATCCTGTCGAAATTAACCTTGAAGGTATTAATCAGGTCAACGTCAATCCAA
AAGTGGGGTTGACTTTCTCCACCGCGCTTAAGTCTTTCTTACGCCAAGATCCTGATATTGTGATGGTTGGTGAGATTCGT
GATCTTGAAACTGCTGAGATTGCGATTAAAGCGGCACAAACAGGCCATATGGTACTTTCAACCTTACATACCAATTCAGC
GCCCGAAACGTTAACGCGATTACGCAATATGGGTGTCGCTTCCTTTAATATTGCTACTTCCGTGAACTTGGTCATTGCTC
AGCGATTGGCACGGCGCTTATGTAAAAACTGTAAAAAACCTATTAGTATCCCACGTCAAAGTCTGCTTGAGCTTGGCTTT
ACAGATGCTGACTTAGACAATACAGACAATATAATCTACGAGCCAGTCGGTTGTAATGAGTGCCGTGAAGGCTACAAAGG
CCGTGTGGGCATTTATGAGGTGATGAAAGTCACTCCAGATATTTCACGTATTATCATGGAAGATGGTAACGCGATAGATA
TCAAAGATGCTGCACTTAAAAACGGTTTTAGAGACTTACGTCGCTCTGGTGTTTTAAAGGTTTTACAAGGCGTCACCAGT
ATTCAAGAAATGATGCGCGTGACTTCTGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

67.895

100

0.679
  pilB Acinetobacter baylyi ADP1

63.732

99.649

0.635
  pilB Legionella pneumophila strain ERS1305867

51.3

100

0.519
  pilF Neisseria gonorrhoeae MS11

49.558

99.123

0.491
  pilB Vibrio cholerae strain A1552

50.654

93.86

0.475
  pilB Vibrio parahaemolyticus RIMD 2210633

51.429

92.105

0.474
  pilB Vibrio campbellii strain DS40M4

49.346

93.86

0.463
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.12

100

0.379