Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N8E86_RS03885 Genome accession   NZ_CP104914
Coordinates   776478..777902 (+) Length   474 a.a.
NCBI ID   WP_264256697.1    Uniprot ID   -
Organism   Avibacterium paragallinarum strain AG21-0333     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 771478..782902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8E86_RS03855 (N8E86_03855) lgt 772053..772853 (-) 801 WP_264256694.1 prolipoprotein diacylglyceryl transferase -
  N8E86_RS03860 (N8E86_03860) - 772863..773654 (-) 792 WP_264256695.1 sulfite exporter TauE/SafE family protein -
  N8E86_RS03865 (N8E86_03865) rppH 773657..774250 (-) 594 WP_264245767.1 RNA pyrophosphohydrolase -
  N8E86_RS03870 (N8E86_03870) - 774822..775241 (-) 420 WP_264256696.1 hypothetical protein -
  N8E86_RS03875 (N8E86_03875) ampD 775340..775894 (-) 555 WP_264245770.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N8E86_RS03880 (N8E86_03880) pilA 776034..776465 (+) 432 WP_035687024.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  N8E86_RS03885 (N8E86_03885) pilB 776478..777902 (+) 1425 WP_264256697.1 GspE/PulE family protein Machinery gene
  N8E86_RS03890 (N8E86_03890) pyrH 778152..778868 (+) 717 WP_035687020.1 UMP kinase -
  N8E86_RS03895 (N8E86_03895) frr 778893..779450 (+) 558 WP_017807054.1 ribosome recycling factor -
  N8E86_RS03900 (N8E86_03900) ispC 779632..780828 (+) 1197 WP_110479821.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
  N8E86_RS03905 (N8E86_03905) uppS 780982..781710 (+) 729 WP_264256698.1 polyprenyl diphosphate synthase -
  N8E86_RS03910 (N8E86_03910) - 781714..782577 (+) 864 WP_264256699.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 53747.50 Da        Isoelectric Point: 5.3266

>NTDB_id=733474 N8E86_RS03885 WP_264256697.1 776478..777902(+) (pilB) [Avibacterium paragallinarum strain AG21-0333]
MADYSLDEDDIVYAVDGKCFEITPHLWQQNKQQKDILIRYLAVPLQEDEHTLWLGIDDLTNLSACESFSFLTGKIVEPIL
LSEQDLKSLLQKLSPTLVEEPALLDFYQSSISPYSENQPEKLDEPIIQILNKVFESAMQLNASDIHLEPQNEQLQIRFRI
DGVLQHQPPISKQMSQRLISRLKLLARLDLSETRLPQDGRFHFKTTFSDILDFRISTLPTYYGEKAVLRLQKNKPVTLDF
SELGMTAIQRKTLQNALSQPQGLILVTGPTGSGKSITLYTALEWLNQSEKHILTAEDPIEIELPGIIQTQINPQIGLDFN
RLLRTFLRQDPDIIMLGEIRDAESAAIALRAAQTGHLVLSTLHTNNAHSAIARLLQLGIQQYEIESCLLLVIAQRLVRKK
CLHKTKNCECYQGYSGRTGIYQFLQAIFDSNTGQISYQTDYPSLWDSASHKIAQGITDIAELERVLGRHTKAEL

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=733474 N8E86_RS03885 WP_264256697.1 776478..777902(+) (pilB) [Avibacterium paragallinarum strain AG21-0333]
ATGGCTGACTATTCTTTAGACGAGGACGACATTGTATATGCTGTTGATGGAAAATGCTTTGAAATAACACCGCACTTATG
GCAACAAAATAAGCAGCAAAAAGATATTTTAATACGTTATTTAGCAGTACCTTTACAGGAGGATGAACATACACTTTGGC
TTGGTATTGATGACTTAACAAATCTATCGGCTTGTGAAAGTTTTTCATTTCTAACAGGAAAAATAGTTGAGCCGATTTTG
TTATCAGAACAAGATTTAAAATCGCTATTACAGAAGTTATCTCCCACATTAGTAGAAGAGCCGGCATTACTTGATTTTTA
TCAATCGTCTATTTCACCTTATAGCGAAAATCAACCAGAAAAACTTGATGAACCAATTATTCAAATACTAAATAAAGTTT
TTGAATCGGCAATGCAATTAAATGCCTCAGATATTCATTTAGAGCCGCAAAATGAGCAATTACAGATCCGCTTCCGTATT
GACGGGGTATTGCAACATCAACCTCCTATTTCTAAACAAATGAGCCAACGTTTGATTTCTCGTTTAAAACTTCTAGCTCG
TCTTGATCTTAGCGAAACTCGCTTACCACAAGATGGAAGATTTCATTTCAAAACGACATTTTCCGATATATTAGACTTCC
GTATATCGACATTACCTACATATTATGGAGAAAAAGCGGTACTACGTTTACAAAAAAATAAACCTGTTACCTTGGATTTT
TCTGAGCTGGGGATGACAGCGATACAACGAAAAACATTACAAAATGCTTTATCTCAACCTCAAGGACTCATTTTAGTAAC
GGGGCCAACTGGGAGTGGAAAAAGTATTACGCTCTATACAGCATTAGAATGGCTTAATCAATCGGAAAAACATATTCTAA
CAGCAGAAGATCCGATTGAAATAGAACTTCCAGGGATTATCCAAACGCAAATTAACCCGCAAATTGGTTTAGATTTTAAC
CGTTTGTTGCGTACATTTTTACGTCAGGATCCAGACATTATTATGCTTGGTGAGATCCGTGATGCAGAAAGTGCAGCGAT
TGCGTTACGTGCAGCACAAACTGGACATTTAGTCCTTTCAACATTACATACAAATAATGCACATTCAGCTATTGCTCGCT
TGTTACAGCTTGGTATTCAACAATATGAAATCGAAAGTTGTTTATTATTAGTGATTGCCCAGCGTTTAGTCCGTAAAAAA
TGTCTACACAAAACGAAAAATTGCGAATGTTATCAAGGTTATAGTGGGCGTACTGGTATTTATCAATTTTTACAAGCTAT
TTTTGATTCAAATACAGGGCAAATTTCTTATCAAACCGATTATCCCTCATTATGGGATAGTGCTTCACATAAAATAGCAC
AAGGTATCACAGATATTGCTGAATTAGAACGAGTTTTAGGTAGGCATACAAAAGCTGAACTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(132-399)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-95)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

64.693

99.789

0.646
  pilB Haemophilus influenzae 86-028NP

63.924

100

0.639
  pilB Glaesserella parasuis strain SC1401

58.952

96.624

0.57
  pilB Legionella pneumophila strain ERS1305867

39.634

100

0.411
  pilB Vibrio campbellii strain DS40M4

40

100

0.409
  pilB Vibrio parahaemolyticus RIMD 2210633

39.794

100

0.407
  pilB Vibrio cholerae strain A1552

39.627

100

0.403
  pilB Acinetobacter baylyi ADP1

37.626

100

0.395
  pilF Neisseria gonorrhoeae MS11

37.317

100

0.376
  pilB Acinetobacter baumannii D1279779

36.495

100

0.373