Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NQS35_RS03365 Genome accession   NZ_CP104494
Coordinates   696015..696524 (+) Length   169 a.a.
NCBI ID   WP_016723977.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain Cw717     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 691015..701524
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQS35_RS03340 (NQS35_03315) - 691360..691986 (+) 627 WP_029240215.1 DUF2889 domain-containing protein -
  NQS35_RS03345 (NQS35_03320) sucC 692053..693219 (+) 1167 WP_011000513.1 ADP-forming succinate--CoA ligase subunit beta -
  NQS35_RS03350 (NQS35_03325) sucD 693304..694185 (+) 882 WP_011000514.1 succinate--CoA ligase subunit alpha -
  NQS35_RS03355 (NQS35_03330) - 694335..695033 (+) 699 WP_020831289.1 TerC family protein -
  NQS35_RS03360 (NQS35_03335) pilA 695227..695748 (+) 522 WP_020831290.1 pilin Machinery gene
  NQS35_RS03365 (NQS35_03340) pilA 696015..696524 (+) 510 WP_016723977.1 pilin Machinery gene
  NQS35_RS03370 (NQS35_03345) - 696607..698340 (+) 1734 WP_016723976.1 PglL family O-oligosaccharyltransferase -
  NQS35_RS03375 (NQS35_03350) moaC 698568..699044 (-) 477 WP_016723975.1 cyclic pyranopterin monophosphate synthase MoaC -
  NQS35_RS03380 (NQS35_03355) - 699138..700826 (+) 1689 WP_069078889.1 tetratricopeptide repeat protein -
  NQS35_RS03385 (NQS35_03360) - 700841..701428 (-) 588 WP_069079304.1 DUF2946 family protein -

Sequence

Protein


Download         Length: 169 a.a.        Molecular weight: 17225.95 Da        Isoelectric Point: 9.9491

>NTDB_id=730026 NQS35_RS03365 WP_016723977.1 696015..696524(+) (pilA) [Ralstonia pseudosolanacearum strain Cw717]
MKSMRHLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTVRARVTEGLSLAAQAKALVSENAANAQSDLSVGSSVFT
ATKNVANLQILGTDATAPGQITVTYTTAAGGGTLALVPTAAGAKLPLSSAPSGPIMWTCYAQNKATAASSVAPSGTMTLA
AKYAPAECR

Nucleotide


Download         Length: 510 bp        

>NTDB_id=730026 NQS35_RS03365 WP_016723977.1 696015..696524(+) (pilA) [Ralstonia pseudosolanacearum strain Cw717]
ATGAAGTCGATGCGTCATCTCAACAAGCGTGTCCAGAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTCTGGCTGCGATTGCCATTCCGGCCTATCAGGACTACACGGTTCGTGCACGCGTGACGGAAGGTCTGTCGCTGG
CTGCACAAGCCAAGGCACTGGTGTCGGAAAACGCTGCCAATGCACAGTCTGACCTGTCGGTGGGTTCGTCGGTGTTCACG
GCCACCAAGAACGTTGCCAACCTCCAGATCCTCGGTACGGATGCTACTGCTCCGGGCCAGATCACCGTTACCTATACGAC
GGCAGCTGGCGGCGGCACGCTGGCTCTGGTTCCGACTGCGGCCGGCGCTAAGCTGCCGTTGAGCTCCGCACCGTCGGGCC
CGATCATGTGGACCTGCTACGCTCAAAACAAGGCCACTGCTGCCAGCAGCGTTGCTCCGAGTGGTACGATGACGCTGGCG
GCCAAGTATGCCCCGGCAGAATGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)

Pilin (bacterial filament) (Pilin)

(43-169)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

93.491

100

0.935
  pilA2 Legionella pneumophila str. Paris

50.617

95.858

0.485
  pilA2 Legionella pneumophila strain ERS1305867

50

95.858

0.479
  comP Acinetobacter baylyi ADP1

43.787

100

0.438
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.979

100

0.42