Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NQS35_RS03360 Genome accession   NZ_CP104494
Coordinates   695227..695748 (+) Length   173 a.a.
NCBI ID   WP_020831290.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain Cw717     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 690227..700748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQS35_RS03335 (NQS35_03310) recX 690606..691085 (+) 480 WP_020831287.1 recombination regulator RecX -
  NQS35_RS03340 (NQS35_03315) - 691360..691986 (+) 627 WP_029240215.1 DUF2889 domain-containing protein -
  NQS35_RS03345 (NQS35_03320) sucC 692053..693219 (+) 1167 WP_011000513.1 ADP-forming succinate--CoA ligase subunit beta -
  NQS35_RS03350 (NQS35_03325) sucD 693304..694185 (+) 882 WP_011000514.1 succinate--CoA ligase subunit alpha -
  NQS35_RS03355 (NQS35_03330) - 694335..695033 (+) 699 WP_020831289.1 TerC family protein -
  NQS35_RS03360 (NQS35_03335) pilA 695227..695748 (+) 522 WP_020831290.1 pilin Machinery gene
  NQS35_RS03365 (NQS35_03340) pilA 696015..696524 (+) 510 WP_016723977.1 pilin Machinery gene
  NQS35_RS03370 (NQS35_03345) - 696607..698340 (+) 1734 WP_016723976.1 PglL family O-oligosaccharyltransferase -
  NQS35_RS03375 (NQS35_03350) moaC 698568..699044 (-) 477 WP_016723975.1 cyclic pyranopterin monophosphate synthase MoaC -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 17811.51 Da        Isoelectric Point: 9.7091

>NTDB_id=730025 NQS35_RS03360 WP_020831290.1 695227..695748(+) (pilA) [Ralstonia pseudosolanacearum strain Cw717]
MASKYSRNRPRGQRGFTLIELMIVVAIIGILAALAIPAYQDYTIRSRVVEGLALAGQAKALVVENAANAQSSLSLGSASL
PASKNVSALNIDSSTGEIAVVYAPAVTEAGANTLVLTPYSGSSSVFGNLQAGSTPPAMVLWVCAAAGKSMPVNTVTQRTT
ATLPPKYAPSECR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=730025 NQS35_RS03360 WP_020831290.1 695227..695748(+) (pilA) [Ralstonia pseudosolanacearum strain Cw717]
ATGGCATCGAAGTACAGCAGGAACCGGCCGCGCGGCCAGCGCGGCTTTACATTGATCGAGTTGATGATCGTGGTGGCGAT
CATTGGGATATTGGCGGCGCTGGCGATTCCGGCGTATCAGGACTACACGATCCGGTCGCGTGTGGTCGAGGGGCTGGCAC
TGGCAGGGCAAGCCAAGGCGCTGGTGGTTGAAAATGCCGCCAACGCACAGAGTAGCCTGTCACTCGGTTCCGCATCGTTG
CCGGCCAGCAAGAACGTCAGTGCGCTGAATATTGATTCGTCCACCGGCGAAATCGCAGTGGTCTATGCACCCGCTGTTAC
GGAAGCGGGGGCGAATACGTTGGTATTGACCCCATATTCCGGCAGTTCGTCAGTATTTGGCAATTTGCAGGCAGGTTCAA
CGCCGCCGGCAATGGTGCTGTGGGTGTGTGCCGCCGCCGGCAAGTCGATGCCGGTCAATACCGTTACCCAGAGGACGACG
GCTACGTTGCCACCGAAATACGCGCCCTCGGAGTGCCGTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(45-173)

Prokaryotic N-terminal methylation motif (N_methyl)

(11-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

51.705

100

0.526
  pilA2 Legionella pneumophila str. Paris

44.848

95.376

0.428
  pilA2 Legionella pneumophila strain ERS1305867

44.242

95.376

0.422
  comP Acinetobacter baylyi ADP1

39.773

100

0.405
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.938

100

0.399