Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   N4S13_RS08155 Genome accession   NZ_CP104392
Coordinates   1626154..1626759 (+) Length   201 a.a.
NCBI ID   WP_028020680.1    Uniprot ID   A0AAW8T3K3
Organism   Enterococcus raffinosus strain HG-5     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1621154..1631759
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4S13_RS08125 (N4S13_08125) - 1621937..1623049 (+) 1113 WP_028020683.1 CAP-associated domain-containing protein -
  N4S13_RS08130 (N4S13_08130) - 1623046..1623471 (+) 426 WP_010744915.1 YlbF family regulator -
  N4S13_RS08135 (N4S13_08135) - 1623458..1623805 (+) 348 WP_028020682.1 YlbG family protein -
  N4S13_RS08140 (N4S13_08140) rsmD 1623965..1624519 (+) 555 WP_010744913.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  N4S13_RS08145 (N4S13_08145) coaD 1624521..1625015 (+) 495 WP_010744912.1 pantetheine-phosphate adenylyltransferase -
  N4S13_RS08150 (N4S13_08150) sepM 1625005..1626048 (+) 1044 WP_028020681.1 SepM family pheromone-processing serine protease Regulator
  N4S13_RS08155 (N4S13_08155) comEA 1626154..1626759 (+) 606 WP_028020680.1 helix-hairpin-helix domain-containing protein Machinery gene
  N4S13_RS08160 (N4S13_08160) - 1626920..1628101 (+) 1182 WP_028020679.1 MFS transporter -
  N4S13_RS08165 (N4S13_08165) - 1628470..1628961 (+) 492 WP_010744907.1 ComE operon protein 2 -
  N4S13_RS08170 (N4S13_08170) - 1629099..1631186 (+) 2088 WP_249024491.1 DNA internalization-related competence protein ComEC/Rec2 -

Sequence

Protein


Download         Length: 201 a.a.        Molecular weight: 22444.38 Da        Isoelectric Point: 4.4221

>NTDB_id=729488 N4S13_RS08155 WP_028020680.1 1626154..1626759(+) (comEA) [Enterococcus raffinosus strain HG-5]
MKEYLKKYPYWLAVPAILAVILFFFFSRTSEEELTDPAVYYSESSKVETSESINQEWYVDVKGAVKKTGMYRISKGMRLM
DAIDLAGGFTAEADQNQINFSKLLSDQEIIYVPKVGEEIPRLKEATVESDGSIGGESQEKININTADATELQKLSGIGEK
KATDIIKYREENGSFNAIDDLTKVSGIGEKTLENLKDSITI

Nucleotide


Download         Length: 606 bp        

>NTDB_id=729488 N4S13_RS08155 WP_028020680.1 1626154..1626759(+) (comEA) [Enterococcus raffinosus strain HG-5]
ATGAAAGAGTATTTAAAAAAGTATCCTTACTGGCTAGCAGTACCGGCTATTCTTGCAGTTATTCTTTTTTTCTTCTTTTC
CAGAACATCTGAAGAAGAGCTAACAGATCCAGCCGTTTATTATTCAGAGAGCAGTAAGGTAGAAACAAGTGAATCTATCA
ATCAAGAGTGGTATGTCGATGTCAAAGGAGCGGTCAAAAAAACAGGAATGTATCGTATTTCAAAAGGCATGCGTCTAATG
GATGCTATTGATCTTGCAGGCGGTTTTACAGCTGAAGCGGATCAGAATCAGATCAATTTTTCAAAGCTTTTGTCGGACCA
AGAAATCATTTATGTACCTAAAGTTGGAGAGGAAATCCCTAGATTAAAAGAAGCAACAGTTGAGTCAGATGGATCAATTG
GAGGGGAATCTCAGGAAAAGATCAACATCAACACAGCGGATGCGACAGAATTGCAGAAATTATCAGGAATTGGTGAGAAA
AAGGCGACGGATATAATCAAATATCGAGAGGAAAATGGCAGCTTCAACGCGATCGATGATCTAACAAAAGTATCCGGCAT
TGGTGAGAAAACGCTTGAAAATTTAAAGGACTCAATTACAATATAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(58-112)

Helix-hairpin-helix motif (HHH_3)

(137-200)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Lactococcus lactis subsp. cremoris KW2

47.847

100

0.498
  comEA Streptococcus thermophilus LMD-9

37.391

100

0.428
  comEA Bacillus subtilis subsp. subtilis str. 168

40

100

0.408
  comEA Staphylococcus aureus MW2

36.364

100

0.398
  comEA Latilactobacillus sakei subsp. sakei 23K

34.632

100

0.398
  comEA Staphylococcus aureus N315

33.182

100

0.363