Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   N4G38_RS02910 Genome accession   NZ_CP104386
Coordinates   619999..621357 (-) Length   452 a.a.
NCBI ID   WP_420070544.1    Uniprot ID   -
Organism   Cupriavidus gilardii strain WM02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 614999..626357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4G38_RS02880 (N4G38_02880) - 615617..616021 (+) 405 WP_053822561.1 NUDIX domain-containing protein -
  N4G38_RS02885 (N4G38_02885) - 616049..616237 (-) 189 WP_053822560.1 DNA gyrase inhibitor YacG -
  N4G38_RS02890 (N4G38_02890) zapD 616244..617002 (-) 759 WP_006578256.1 cell division protein ZapD -
  N4G38_RS02895 (N4G38_02895) coaE 617253..617885 (-) 633 WP_053822559.1 dephospho-CoA kinase -
  N4G38_RS02900 (N4G38_02900) - 617898..618839 (-) 942 WP_420070543.1 prepilin peptidase -
  N4G38_RS02905 (N4G38_02905) - 618841..619905 (-) 1065 WP_260731017.1 type II secretion system F family protein -
  N4G38_RS02910 (N4G38_02910) pilF 619999..621357 (-) 1359 WP_420070544.1 GspE/PulE family protein Machinery gene
  N4G38_RS02920 (N4G38_02920) ispB 621520..622449 (-) 930 WP_053822557.1 octaprenyl diphosphate synthase -
  N4G38_RS02925 (N4G38_02925) rplU 622993..623304 (+) 312 WP_006576525.1 50S ribosomal protein L21 -
  N4G38_RS02930 (N4G38_02930) rpmA 623368..623628 (+) 261 WP_053822556.1 50S ribosomal protein L27 -
  N4G38_RS02935 (N4G38_02935) obgE 623766..624863 (+) 1098 WP_053822555.1 GTPase ObgE -
  N4G38_RS02940 (N4G38_02940) proB 624926..626050 (+) 1125 WP_064577249.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 48762.40 Da        Isoelectric Point: 8.3207

>NTDB_id=729289 N4G38_RS02910 WP_420070544.1 619999..621357(-) (pilF) [Cupriavidus gilardii strain WM02]
MLWPSPPFETGRIPVPAGASHTGPLPHCVPSAMIAMTAPSDAPIEYDPAMPSADDAPVVRFVQTLLAEACRRGASDLHFE
PYEGFYRVRFRIDGQLHEVARPPLDIRDRIATRIKVLARLDIAEKRVPQDGRMRFVVPRRDGEATPDGAAGRAVDLRVST
LPTLFGEKIVLRMLAASSVRLDMDSLGLEPTQLAVLLERLRRPHGMVLVTGPTGSGKTVSLYAFLNLLNQHERNISTAED
PAEIQLAGINQVNIHDKIGLNFPTVLRALLRQDPDVIMVGEIRDAETADVAVKAAQTGHLVLSTLHTNDAPGTLTRLAQL
GVAPFHLAASVLVITAQRLARMLCACKRPVPVPLSALREAGFADTLLDGDWQPCQPVGCAACRGSGYRGRCGIHQVMPVS
DAMQNIILSRGSARALAQQARREGVLSLREAGLLKVRAGTTSLAEVLATTNP

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=729289 N4G38_RS02910 WP_420070544.1 619999..621357(-) (pilF) [Cupriavidus gilardii strain WM02]
TTGCTTTGGCCGTCCCCGCCATTTGAGACTGGGCGCATTCCCGTTCCCGCCGGCGCATCGCACACTGGGCCGCTCCCGCA
CTGCGTCCCGTCCGCCATGATCGCCATGACCGCCCCGTCCGATGCGCCGATCGAATACGACCCCGCCATGCCATCGGCCG
ACGACGCGCCGGTCGTGCGCTTCGTCCAGACGCTGCTGGCCGAGGCCTGCCGGCGCGGCGCCTCGGACCTGCACTTCGAG
CCGTACGAAGGCTTCTATCGCGTGCGCTTTCGCATCGATGGGCAGCTGCACGAGGTCGCCCGTCCGCCGCTGGACATTCG
CGATCGCATCGCGACGCGGATCAAGGTGCTGGCGCGGCTGGACATCGCCGAGAAGCGTGTACCGCAGGATGGCCGCATGC
GGTTCGTGGTGCCGCGGCGCGACGGCGAGGCGACGCCTGACGGGGCCGCCGGCCGCGCGGTCGATCTGCGCGTATCGACG
CTGCCGACGCTGTTCGGCGAAAAGATCGTGCTGCGCATGCTGGCCGCGTCCAGCGTGCGGCTCGATATGGACAGCCTCGG
CCTCGAGCCGACGCAGCTGGCCGTGCTGCTCGAGCGGCTGCGCCGTCCGCACGGCATGGTGCTGGTCACCGGTCCGACCG
GCAGCGGCAAGACCGTTTCGCTGTACGCCTTCCTGAATCTGCTGAACCAGCACGAGCGCAATATCTCGACGGCCGAGGAC
CCGGCCGAGATCCAGCTGGCCGGCATCAACCAGGTCAATATCCACGACAAGATTGGCCTGAACTTCCCCACCGTGCTGCG
AGCGTTGCTGCGGCAGGACCCCGACGTGATCATGGTCGGCGAGATCCGCGATGCCGAAACCGCCGATGTCGCGGTCAAGG
CGGCGCAGACCGGCCATCTGGTCCTGTCGACGCTGCACACCAACGATGCGCCGGGCACGCTGACACGGCTCGCGCAGCTT
GGCGTCGCGCCCTTTCACCTGGCCGCCAGCGTGCTGGTGATCACCGCGCAGCGGCTGGCCCGCATGCTGTGCGCCTGCAA
ACGTCCGGTCCCGGTGCCGCTGTCCGCGCTGCGCGAGGCCGGTTTCGCGGACACCTTGCTCGACGGCGACTGGCAGCCCT
GCCAGCCGGTCGGCTGCGCCGCGTGCCGCGGCAGCGGTTATCGCGGGCGCTGCGGCATCCATCAGGTGATGCCAGTCTCC
GACGCGATGCAGAACATCATCCTGTCGCGGGGCAGTGCCCGTGCGCTGGCCCAGCAGGCGCGCCGCGAGGGCGTGCTATC
GTTGCGCGAGGCGGGGCTGCTCAAGGTCAGGGCGGGCACGACCTCGCTGGCCGAGGTGCTGGCGACGACCAATCCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(65-342)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

57.107

88.717

0.507
  pilB Acinetobacter baumannii D1279779

53.465

89.381

0.478
  pilB Acinetobacter baylyi ADP1

54.198

86.947

0.471
  pilB Vibrio cholerae strain A1552

52.736

88.938

0.469
  pilB Legionella pneumophila strain ERS1305867

49.635

90.929

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

51.01

87.611

0.447
  pilB Vibrio campbellii strain DS40M4

50.758

87.611

0.445
  pilB/pilB1 Synechocystis sp. PCC 6803

42.584

92.478

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.68

89.159

0.381
  pilF Thermus thermophilus HB27

41.96

88.053

0.369