Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   N4T19_RS17965 Genome accession   NZ_CP104377
Coordinates   3912717..3913250 (+) Length   177 a.a.
NCBI ID   WP_313900069.1    Uniprot ID   -
Organism   Comamonas sp. PR12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3907717..3918250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4T19_RS17940 (N4T19_17940) sucC 3908588..3909748 (+) 1161 WP_021026617.1 ADP-forming succinate--CoA ligase subunit beta -
  N4T19_RS17945 (N4T19_17945) sucD 3909765..3910661 (+) 897 WP_021026616.1 succinate--CoA ligase subunit alpha -
  N4T19_RS17950 (N4T19_17950) - 3910774..3911457 (+) 684 WP_116926062.1 TerC family protein -
  N4T19_RS17955 (N4T19_17955) - 3911757..3912404 (+) 648 WP_260718684.1 FHA domain-containing protein -
  N4T19_RS17965 (N4T19_17965) pilE 3912717..3913250 (+) 534 WP_313900069.1 pilin Machinery gene
  N4T19_RS17970 (N4T19_17970) - 3913342..3914088 (+) 747 WP_260718685.1 hypothetical protein -
  N4T19_RS17975 (N4T19_17975) moaC 3914108..3914590 (-) 483 WP_260718686.1 cyclic pyranopterin monophosphate synthase MoaC -
  N4T19_RS17980 (N4T19_17980) - 3914791..3916488 (+) 1698 WP_313900070.1 M48 family metalloprotease -
  N4T19_RS17985 (N4T19_17985) - 3916456..3916812 (-) 357 WP_021026611.1 phage holin family protein -
  N4T19_RS17990 (N4T19_17990) - 3916944..3917384 (-) 441 WP_021026610.1 hypothetical protein -
  N4T19_RS17995 (N4T19_17995) - 3917452..3918195 (-) 744 WP_021026609.1 TerC family protein -

Sequence

Protein


Download         Length: 177 a.a.        Molecular weight: 18199.81 Da        Isoelectric Point: 7.7786

>NTDB_id=729229 N4T19_RS17965 WP_313900069.1 3912717..3913250(+) (pilE) [Comamonas sp. PR12]
MLRKMQKGFTLIELMIVVAIIGILAAIALPAYQDYTIRTKISEGVIAGSSAKGLMSEAFQTDGTAGLTAAATAYMANPNQ
KAEASSKFVDDVTIATDTPWNITVAIKATAGNGIPTGLNTQTITFTPNVQNAAPTAASKGAIDWACASDSNQTATSRGLA
SVVAGTLPAKYAPAECR

Nucleotide


Download         Length: 534 bp        

>NTDB_id=729229 N4T19_RS17965 WP_313900069.1 3912717..3913250(+) (pilE) [Comamonas sp. PR12]
ATGCTGCGTAAGATGCAAAAGGGTTTCACCCTGATCGAACTGATGATCGTTGTGGCGATTATCGGTATTTTGGCTGCTAT
CGCACTGCCTGCTTATCAAGATTACACTATTCGTACGAAGATTTCGGAAGGCGTCATTGCGGGCAGCTCTGCTAAAGGTT
TGATGTCTGAAGCGTTTCAGACGGATGGCACCGCAGGATTGACCGCTGCTGCCACTGCCTACATGGCAAATCCAAATCAA
AAGGCTGAAGCTTCGTCAAAGTTCGTGGATGATGTCACTATTGCTACTGATACTCCATGGAATATCACTGTCGCAATCAA
AGCAACCGCAGGCAATGGTATTCCAACTGGATTGAATACCCAGACTATTACTTTCACGCCCAACGTGCAGAATGCAGCTC
CTACCGCTGCATCCAAAGGCGCTATTGACTGGGCTTGTGCTTCGGATTCTAATCAAACTGCGACCAGTCGTGGTCTTGCA
AGCGTAGTTGCTGGTACACTTCCTGCAAAGTACGCTCCTGCAGAATGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(38-177)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

57.979

100

0.616
  pilA2 Legionella pneumophila strain ERS1305867

42.286

98.87

0.418
  pilA2 Legionella pneumophila str. Paris

42.286

98.87

0.418
  pilA Ralstonia pseudosolanacearum GMI1000

41.111

100

0.418
  pilA/pilA1 Eikenella corrodens VA1

40.237

95.48

0.384
  comP Acinetobacter baylyi ADP1

36.158

100

0.362