Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N4T19_RS11695 Genome accession   NZ_CP104377
Coordinates   2517395..2519128 (+) Length   577 a.a.
NCBI ID   WP_116927081.1    Uniprot ID   A0A363RHC4
Organism   Comamonas sp. PR12     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2512395..2524128
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4T19_RS11670 (N4T19_11670) proB 2512917..2514056 (-) 1140 WP_116927078.1 glutamate 5-kinase -
  N4T19_RS11675 (N4T19_11675) obgE 2514141..2515256 (-) 1116 WP_260720246.1 Obg family GTPase CgtA -
  N4T19_RS11680 (N4T19_11680) rpmA 2515446..2515706 (-) 261 WP_021027814.1 50S ribosomal protein L27 -
  N4T19_RS11685 (N4T19_11685) rplU 2515722..2516033 (-) 312 WP_027011098.1 50S ribosomal protein L21 -
  N4T19_RS11690 (N4T19_11690) - 2516301..2517230 (+) 930 WP_260720291.1 polyprenyl synthetase family protein -
  N4T19_RS11695 (N4T19_11695) pilB 2517395..2519128 (+) 1734 WP_116927081.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N4T19_RS11700 (N4T19_11700) pilC 2519149..2520369 (+) 1221 WP_116927082.1 type II secretion system F family protein Machinery gene
  N4T19_RS11705 (N4T19_11705) - 2520372..2521247 (+) 876 WP_182343457.1 prepilin peptidase -
  N4T19_RS11710 (N4T19_11710) coaE 2521249..2521878 (+) 630 WP_260720247.1 dephospho-CoA kinase -
  N4T19_RS11715 (N4T19_11715) zapD 2521882..2522637 (+) 756 WP_116927085.1 cell division protein ZapD -
  N4T19_RS11720 (N4T19_11720) - 2522699..2522911 (+) 213 WP_116927086.1 DNA gyrase inhibitor YacG -
  N4T19_RS11725 (N4T19_11725) - 2522959..2523432 (-) 474 WP_260720248.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62664.07 Da        Isoelectric Point: 5.2339

>NTDB_id=729219 N4T19_RS11695 WP_116927081.1 2517395..2519128(+) (pilB) [Comamonas sp. PR12]
MPDINTPPKAVALPGIARALVASGKLPVAKAEALYATSLKSKNSFIGELVASGAVSGSDVASIISTVYGAPLVDLDAIDL
TRLATGLLDPKLALSNKIIPLAKRGNRLIVVTADPTDHSVAEQIKFSTQLSVDWVIAEHSKLVKALESVAKKGGDNIQDM
ITGDVNLNEFEIDDESVNTDTASNDGDEVEDAPIVKFLQKMLIDAFNMRASDLHFEPYEHQYRIRFRIDGELREIASPPP
GIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGADRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKER
LLSAIKRPYGMILVTGPTGSGKTVSLYTCLNILNTPAVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKSFLRQDPD
IIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARKLCPICKRPTEIPH
EALLEAGFKEEDLDGTWMPYEPVGCDACNNGYKGRLGIYQVMPISEETQRIILRDGSALEIAEQADIENVRSLRTSGLHK
VKKGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=729219 N4T19_RS11695 WP_116927081.1 2517395..2519128(+) (pilB) [Comamonas sp. PR12]
ATGCCTGACATCAACACCCCCCCCAAAGCCGTCGCCCTCCCCGGCATTGCCAGAGCGCTGGTAGCCAGTGGAAAGCTTCC
TGTTGCCAAGGCGGAGGCCTTGTACGCCACCAGTCTCAAAAGCAAGAACAGCTTCATTGGAGAGCTGGTGGCCAGTGGTG
CCGTCTCCGGCAGCGATGTAGCCAGCATCATCTCGACGGTCTATGGCGCGCCGCTGGTCGACCTCGATGCTATCGATTTG
ACCCGGCTGGCAACAGGTTTGCTTGATCCCAAACTGGCGCTCAGCAACAAGATCATTCCCCTGGCCAAACGCGGCAACCG
GCTGATCGTCGTTACCGCCGACCCGACCGACCACTCGGTGGCCGAGCAGATCAAGTTCTCCACCCAGCTGAGCGTGGACT
GGGTGATTGCAGAACATTCCAAGCTGGTCAAGGCGCTGGAGTCTGTTGCGAAAAAGGGCGGCGACAACATCCAGGACATG
ATCACCGGCGATGTCAACCTCAATGAATTCGAGATTGATGATGAGTCGGTGAACACCGACACCGCGAGCAATGACGGAGA
TGAGGTCGAAGATGCGCCCATCGTCAAGTTTTTGCAGAAGATGCTGATTGACGCATTCAATATGCGTGCATCGGACTTGC
ATTTCGAACCCTATGAGCACCAGTACCGCATCCGCTTTCGCATCGATGGAGAGCTGCGCGAAATCGCTTCGCCGCCCCCG
GGCATCAAGGACAAGCTGGCATCGCGGATCAAGGTGATCTCGCGCCTGGATATCTCGGAAAAGCGCGTGCCGCAGGACGG
CCGCATGAAGCTCAAGATCGGCGCGGATCGGGTGATCGATTTCCGGGTCAGCACCTTGCCCACCTTGTTTGGCGAAAAGA
TCGTGATCCGTATTCTCGATCCGAGCAGCGCCAAGCTGGGCATTGATGCACTGGGCTATGAGCCCGAAGAGAAGGAGCGC
CTGCTCAGCGCCATCAAGCGCCCTTACGGCATGATTCTGGTCACGGGGCCCACGGGTTCGGGCAAGACCGTGTCGCTCTA
CACCTGCCTGAATATTCTCAATACGCCAGCGGTCAATATAGCGACTGCCGAAGACCCGTCGGAAATCAACCTGCCCGGGG
TCAACCAGGTCAATGTCAATGAAAAGGCCGGTCTGACCTTTGCGACCGCGCTCAAGTCCTTTCTGCGCCAGGATCCCGAC
ATCATCATGGTCGGTGAAATCCGTGACCTGGAGACGGCCGACATCGCCATCAAGGCGGCGCAGACCGGTCACTTGGTGCT
GTCGACCCTGCACACCAACGACGCCCCCACCACCTTGACGCGTATGCGCAATATGGGGATCGCGCCCTTCAATATCGCGT
CGAGCGTGATCTTGATCACCGCGCAGCGCCTGGCACGCAAGCTCTGCCCGATCTGCAAGCGCCCCACCGAAATCCCCCAT
GAAGCGCTGCTGGAAGCGGGCTTCAAGGAAGAGGACCTGGATGGCACCTGGATGCCCTACGAGCCCGTTGGCTGCGACGC
CTGCAACAATGGCTACAAGGGCCGCCTGGGCATCTACCAAGTGATGCCCATCTCCGAAGAAACACAGCGCATCATCTTGC
GCGATGGCAGCGCACTGGAAATCGCCGAGCAAGCCGACATAGAAAATGTCCGCTCCTTGCGCACGTCAGGCCTGCACAAA
GTCAAAAAGGGACTGACCTCACTCGAAGAGGTCTTAGCCGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A363RHC4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.75

100

0.549
  pilB Acinetobacter baumannii D1279779

54.202

100

0.548
  pilF Neisseria gonorrhoeae MS11

53.004

98.094

0.52
  pilB Legionella pneumophila strain ERS1305867

50.265

98.267

0.494
  pilB Vibrio cholerae strain A1552

46.032

98.267

0.452
  pilB Vibrio campbellii strain DS40M4

45.439

98.787

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

45.633

97.227

0.444
  pilF Thermus thermophilus HB27

38.879

92.721

0.36