Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MRX62_RS07890 Genome accession   NZ_CP104027
Coordinates   1644062..1645795 (-) Length   577 a.a.
NCBI ID   WP_060871718.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. pauca strain 50088     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1639062..1650795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRX62_RS07875 (MRX62_07865) sucC 1639348..1640511 (-) 1164 WP_046418841.1 ADP-forming succinate--CoA ligase subunit beta -
  MRX62_RS07880 (MRX62_07870) - 1640760..1642370 (+) 1611 WP_060871720.1 PAS domain-containing sensor histidine kinase -
  MRX62_RS07885 (MRX62_07875) pilR 1642556..1643968 (+) 1413 WP_060871719.1 sigma-54 dependent transcriptional regulator Regulator
  MRX62_RS07890 (MRX62_07880) pilB 1644062..1645795 (-) 1734 WP_060871718.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MRX62_RS07895 (MRX62_07885) - 1646636..1647103 (-) 468 WP_023907060.1 pilin -
  MRX62_RS07900 (MRX62_07890) - 1648401..1648847 (-) 447 WP_060871716.1 pilin -
  MRX62_RS07905 (MRX62_07895) - 1649178..1649357 (-) 180 WP_195780022.1 hypothetical protein -
  MRX62_RS07910 (MRX62_07900) pilC 1649287..1650489 (+) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62992.06 Da        Isoelectric Point: 5.9154

>NTDB_id=727128 MRX62_RS07890 WP_060871718.1 1644062..1645795(-) (pilB) [Xylella fastidiosa subsp. pauca strain 50088]
MNTPHSANLVGITGIARRLVQDGSIEEATARTAQNQAMAAKVPLPQWLIDKKLVSTAKLAAANALEFGMPLLDVSAFDHN
HNAMSLVDEELLQKHRVLPLFKRGNRLFVGISNPTQTQALDDIKFHTNLVVEPILVDEDQIRRTLEQWQSRSGALSSALE
EEDNGIEGLDIGGGDDDGASGDTGIDAKSDDTPVVKFVNKVLIDAIRRGASDIHFEPYEEDYRVRFRIDGLLKAVAKAPN
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQVDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQTL
FMEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
VIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNKCKRPVQLPT
NALLAEGFTQEQIDAGLELYEPVGCDECTEGYKGRTGIYQVMPITEGISSIILAGGNALQIAEEAQRIGINDLRQSALLK
VAQGITGLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=727128 MRX62_RS07890 WP_060871718.1 1644062..1645795(-) (pilB) [Xylella fastidiosa subsp. pauca strain 50088]
ATGAACACACCTCATTCAGCCAATCTTGTCGGCATCACCGGCATTGCCCGTCGCCTAGTCCAGGATGGCAGCATCGAGGA
AGCCACCGCACGCACTGCTCAAAATCAAGCAATGGCCGCAAAAGTGCCTTTACCACAGTGGTTGATCGATAAAAAACTAG
TAAGTACCGCAAAGTTGGCCGCAGCCAATGCACTTGAGTTCGGGATGCCGCTGCTGGATGTCTCCGCATTCGACCACAAT
CACAATGCAATGAGCTTGGTCGACGAAGAATTACTGCAAAAGCATCGGGTGTTGCCATTATTTAAACGCGGTAATCGACT
GTTCGTCGGGATCAGCAATCCAACCCAAACACAGGCCCTGGACGACATCAAATTCCACACCAATCTGGTCGTGGAACCGA
TTCTGGTCGACGAAGACCAGATACGACGCACACTTGAACAATGGCAATCTAGGAGCGGCGCGCTCAGCTCCGCATTGGAG
GAAGAGGATAACGGCATTGAAGGGCTGGATATCGGCGGCGGAGACGACGACGGGGCCAGTGGCGATACAGGGATTGATGC
CAAGAGCGATGACACACCTGTCGTCAAATTCGTCAACAAAGTGCTGATTGATGCCATCCGACGCGGCGCATCAGACATCC
ACTTTGAACCATACGAAGAAGATTACCGCGTCCGTTTCCGGATTGATGGTCTGCTGAAGGCTGTGGCAAAAGCACCGAAC
AAGTTGAATCAGCGTATCGCCGCACGCTTGAAAGTCATGTCGCAACTGGATATCGCGGAAAAACGCGTCCCTCAAGACGG
ACGTATCAAGCTTAATCTGTCGAAAACCAAGCAAGTCGATTTCCGCGTCAGCACCTTGCCAACGCTGTTCGGCGAGAAAG
TGGTACTGCGTATCTTGGATGGCAGCGCGGCAAAGCTGGGTATCGACAAACTCGGTTACGAAGCTGACCAACAAACGCTT
TTTATGGAAGCAATCCACAAACCCTATGGAATGGTATTGGTCACTGGTCCGACCGGTTCGGGCAAGACAGTGTCACTGTA
CACTGCACTAGGCATCCTCAATGACGAAACACGCAATATCTCCACAGCCGAAGATCCTGTAGAAATCCGGCTGCCGGGCG
TCAATCAGGTACAACAAAACAACAAGCGCGGTATGACGTTTGCCGCAGCATTACGTTCGTTTCTGCGCCAAGACCCGGAT
GTCATCATGGTCGGCGAAATCCGGGATCTGGAAACCGCTGAAATCGCCATCAAAGCCGCACAGACCGGCCACATGGTGCT
CTCCACACTACATACCAACGATGCACCGCAAACCATCGCACGCCTCATGAACATGGGGATTGCACCATACAACATCACTT
CATCCGTGACCTTAGTGATTGCACAGCGATTGGCACGGAGATTGTGCAACAAGTGCAAGCGCCCGGTTCAATTGCCGACA
AACGCATTACTGGCCGAAGGATTCACTCAAGAACAAATTGATGCGGGGCTGGAATTATACGAGCCTGTCGGTTGCGACGA
ATGCACCGAAGGCTACAAAGGCCGCACAGGTATTTATCAAGTGATGCCAATTACCGAAGGGATTTCCTCGATCATTTTAG
CCGGCGGTAATGCCTTACAAATCGCCGAAGAGGCGCAGCGCATCGGCATCAACGACCTACGCCAATCGGCGCTACTCAAA
GTCGCTCAGGGCATCACCGGGCTGGCTGAAATTAATCGAGTCACCAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.143

98.267

0.562
  pilB Acinetobacter baumannii D1279779

56.437

98.267

0.555
  pilB Legionella pneumophila strain ERS1305867

51.761

98.44

0.51
  pilF Neisseria gonorrhoeae MS11

50.439

98.614

0.497
  pilB Vibrio cholerae strain A1552

49.724

94.107

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

47.08

97.92

0.461
  pilB Vibrio campbellii strain DS40M4

46.737

98.267

0.459
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.993

92.894

0.362
  pilF Thermus thermophilus HB27

40.077

89.948

0.36