Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NYP20_RS12925 Genome accession   NZ_CP103965
Coordinates   2798785..2799894 (+) Length   369 a.a.
NCBI ID   WP_259503167.1    Uniprot ID   -
Organism   Pseudomonas sp. N3-W     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2793785..2804894
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYP20_RS12910 (NYP20_12910) - 2794163..2795080 (+) 918 WP_259502695.1 sugar kinase -
  NYP20_RS12915 (NYP20_12915) - 2795791..2796066 (-) 276 WP_007898282.1 peptidylprolyl isomerase -
  NYP20_RS12920 (NYP20_12920) - 2796116..2798653 (-) 2538 WP_259502696.1 PAS domain-containing sensor histidine kinase -
  NYP20_RS12925 (NYP20_12925) pilU 2798785..2799894 (+) 1110 WP_259503167.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NYP20_RS12930 (NYP20_12930) - 2799940..2801895 (-) 1956 WP_259502697.1 acetoacetate--CoA ligase -
  NYP20_RS12935 (NYP20_12935) hbdH 2802076..2802849 (-) 774 WP_259502698.1 3-hydroxybutyrate dehydrogenase -
  NYP20_RS12940 (NYP20_12940) - 2802857..2804248 (-) 1392 WP_259502699.1 GntP family permease -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41131.07 Da        Isoelectric Point: 6.3760

>NTDB_id=726670 NYP20_RS12925 WP_259503167.1 2798785..2799894(+) (pilU) [Pseudomonas sp. N3-W]
MEIDVLLRQLASQDGSDLYLATGAPPSARFDGVLRALSEERFKPGEIAAIAASIMDAEQRLTFDRELEMNLAISLAGIGR
FRINIFRQRNDVSIVARNVKLDVPRFEDLKLPSILLESVMLKQGLILFVGATDSGKSTSLAALIDYRNRHSSGHIITIED
PVEYIHRHKRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRI
LNFFPEERRAQLLHDLANNLKAFVSQRLVRTLDGQRRAAVEVMLGTPTIADLILRNEFGELKTIMEKSLELGMQTFDGAL
FGLVVEGAISEAEALKHADSVNNLRLRLKLHADSSPTSQRPPGDWDLMD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=726670 NYP20_RS12925 WP_259503167.1 2798785..2799894(+) (pilU) [Pseudomonas sp. N3-W]
ATGGAAATCGACGTGTTGTTGCGCCAGTTGGCCAGTCAGGATGGCTCTGATCTTTACCTTGCTACCGGTGCGCCGCCCAG
TGCGCGGTTCGACGGCGTACTCAGGGCCTTGAGTGAAGAACGGTTCAAACCGGGCGAAATCGCCGCCATCGCGGCGTCGA
TCATGGATGCCGAACAGCGTCTGACGTTCGACCGGGAGCTGGAAATGAACCTGGCGATTTCCCTGGCCGGCATCGGGCGC
TTCCGGATCAACATCTTCAGGCAGCGTAACGACGTGTCGATCGTGGCGCGCAACGTCAAGCTGGACGTGCCGCGTTTCGA
AGACCTCAAGTTGCCGTCGATACTGCTTGAGAGCGTGATGCTCAAACAAGGGCTGATCCTGTTCGTCGGCGCCACCGATT
CGGGTAAATCGACCTCGCTGGCGGCGTTGATCGATTACCGCAACCGCCACAGTAGCGGCCATATCATCACCATCGAAGAC
CCGGTGGAGTACATCCATCGGCACAAGCGCTCGATCATCAATCAGCGGGAAGTGGGTGTCGACACCCGCAGTTTTCACGC
GGCGTTGAAGAACACCCTGCGCCAGGCACCGGACGTGGTACTGATCGGCGAAATTCGCGACCGCGAAACCATGGAGCACG
CGCTGGCGTTTGCCGATACTGGCCACCTGGTGATTTCCACCCTGCATGCGCACAACGCCAACCAGGCGCTGGACCGCATC
CTCAACTTCTTTCCCGAAGAACGCCGTGCGCAGTTGCTGCATGACCTGGCCAACAACCTCAAGGCCTTTGTGTCCCAGCG
CCTGGTACGCACCCTCGACGGCCAGCGCCGGGCGGCGGTAGAGGTGATGCTGGGGACCCCGACCATCGCCGACCTGATCT
TGCGCAACGAGTTTGGCGAGCTCAAGACCATCATGGAAAAATCGCTGGAACTGGGGATGCAGACGTTTGACGGGGCGCTG
TTCGGGCTGGTGGTGGAGGGCGCGATCAGCGAGGCGGAGGCGTTGAAGCATGCCGATTCGGTGAATAATCTGCGGTTGCG
ATTGAAGCTGCATGCGGATTCGTCACCGACATCCCAGCGCCCGCCCGGCGACTGGGATCTGATGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.366

96.206

0.523
  pilU Acinetobacter baylyi ADP1

52.101

96.748

0.504
  pilU Vibrio cholerae strain A1552

51.429

94.851

0.488
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Pseudomonas stutzeri DSM 10701

39.244

93.225

0.366
  pilT Pseudomonas aeruginosa PAK

39.244

93.225

0.366