Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR19_RS10220 Genome accession   NZ_CP103874
Coordinates   2176270..2177679 (-) Length   469 a.a.
NCBI ID   WP_302508102.1    Uniprot ID   -
Organism   Gallibacterium anatis strain TH22     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2171270..2182679
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR19_RS10185 (NYR19_10195) - 2171556..2172215 (+) 660 WP_302508092.1 formate dehydrogenase subunit gamma -
  NYR19_RS10190 (NYR19_10200) fdhE 2172217..2173140 (+) 924 WP_302508094.1 formate dehydrogenase accessory protein FdhE -
  NYR19_RS10195 (NYR19_10205) - 2173206..2173478 (-) 273 WP_018345916.1 GNAT family N-acetyltransferase -
  NYR19_RS10200 (NYR19_10210) yacG 2173562..2173780 (-) 219 WP_018345915.1 DNA gyrase inhibitor YacG -
  NYR19_RS10205 (NYR19_10215) coaE 2173770..2174402 (-) 633 WP_302508097.1 dephospho-CoA kinase -
  NYR19_RS10210 (NYR19_10220) - 2174417..2175049 (-) 633 WP_230586435.1 prepilin peptidase -
  NYR19_RS10215 (NYR19_10225) - 2175046..2176254 (-) 1209 WP_302508101.1 type II secretion system F family protein -
  NYR19_RS10220 (NYR19_10230) pilB 2176270..2177679 (-) 1410 WP_302508102.1 GspE/PulE family protein Machinery gene
  NYR19_RS10225 (NYR19_10235) pilA 2177696..2178157 (-) 462 WP_013746605.1 prepilin peptidase-dependent pilin Machinery gene
  NYR19_RS10230 (NYR19_10240) ampD 2178262..2178816 (+) 555 WP_018345909.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NYR19_RS10235 (NYR19_10245) - 2178903..2179238 (+) 336 WP_013746603.1 hypothetical protein -
  NYR19_RS10240 (NYR19_10250) - 2179347..2180741 (+) 1395 WP_302508103.1 chloride channel protein -
  NYR19_RS10245 (NYR19_10255) dusA 2180722..2181702 (+) 981 WP_302508104.1 tRNA dihydrouridine(20/20a) synthase DusA -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 52831.20 Da        Isoelectric Point: 5.1780

>NTDB_id=726326 NYR19_RS10220 WP_302508102.1 2176270..2177679(-) (pilB) [Gallibacterium anatis strain TH22]
MGYCVKALDSEQLFEISELQWQKNQQQYELLLRYLAVPLQEQAQQLWLAVESFDNLAACETFAFLSGKQIEPVAIGQTAL
KQLLQQLQPGTATNQVQESQLHSYQQNDIVAKSDEIAEVDAQEPIIQLFNQLLESGLQYSVSDIHLEPLRQEFQIRFRID
GVLQRHQVLTLSIGQRLNSRLKLLAKLDINELRLPQDGRFRFKTAFGETLDIRLSSLPTQYGEKIVLRLQPNTPVFDDFT
ELGMTNEQAVLFRQALSQPQGLILVTGPTGSGKSLTLYSGLHFLNQESRHILTAEDPIELQLNGIIQTQANAAIGLTFAT
LLRSFLRQDPDVIMVGEIRDQETAEIALRAAQTGHLVLSTLHTNTAIGAISRLQQLGIAQHELENSLLLVVAQRLVRKRC
NLCQGDKSQQCDCVDGYRGRTGVYQLLHYQQQQIQMDYPTLLAAAQLKLQQQITTTAEVERVFGKTENS

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=726326 NYR19_RS10220 WP_302508102.1 2176270..2177679(-) (pilB) [Gallibacterium anatis strain TH22]
ATGGGCTATTGTGTAAAGGCATTGGATTCGGAGCAGCTGTTTGAAATTTCAGAATTGCAATGGCAGAAGAATCAACAGCA
ATATGAACTGTTATTGCGTTATTTGGCGGTACCGTTGCAGGAACAGGCGCAACAGTTATGGTTAGCGGTTGAGTCTTTCG
ATAATTTAGCCGCTTGCGAAACCTTTGCTTTTCTTTCCGGCAAACAGATTGAGCCTGTTGCGATCGGACAAACTGCACTG
AAACAGTTATTACAGCAGCTGCAACCCGGAACGGCGACAAATCAAGTGCAAGAATCACAACTGCATAGTTATCAGCAAAA
TGATATTGTCGCAAAATCGGATGAAATCGCGGAAGTTGATGCACAAGAGCCGATTATTCAACTATTCAATCAATTATTGG
AATCAGGTTTGCAATATTCCGTTTCTGATATTCATCTTGAGCCGTTACGTCAGGAGTTCCAGATCCGCTTTAGAATTGAC
GGCGTATTACAACGGCATCAGGTGCTGACGTTGTCAATAGGTCAACGCTTAAACTCACGCCTAAAATTGTTGGCAAAACT
GGATATTAACGAATTACGCTTGCCGCAGGATGGTCGTTTTCGTTTTAAAACCGCATTCGGTGAAACATTAGATATTCGGT
TATCTTCTCTACCGACACAATATGGTGAAAAAATCGTATTACGATTGCAACCTAACACACCGGTGTTTGATGATTTTACC
GAACTGGGAATGACAAACGAGCAGGCTGTGCTGTTTCGGCAAGCGTTATCTCAACCGCAAGGATTAATCTTGGTTACCGG
ACCGACCGGAAGTGGTAAAAGTTTGACATTATATAGCGGTTTACATTTTTTAAATCAGGAAAGTCGCCACATTCTTACAG
CGGAAGATCCGATAGAACTGCAATTAAACGGTATTATTCAGACCCAAGCAAATGCCGCAATCGGTTTAACTTTTGCAACA
TTATTGCGGAGTTTTTTACGCCAAGATCCGGATGTCATTATGGTTGGGGAAATTCGAGATCAGGAAACGGCAGAGATAGC
ATTACGCGCGGCACAGACGGGACATTTAGTGTTATCGACACTGCATACCAATACGGCAATCGGTGCAATTTCCCGATTAC
AGCAGTTGGGAATTGCGCAACACGAATTGGAAAATTCATTATTGTTGGTGGTGGCACAACGTTTAGTCCGCAAACGTTGT
AACTTATGCCAAGGTGATAAATCTCAGCAATGCGATTGTGTTGATGGTTATCGCGGACGAACCGGCGTTTATCAACTGCT
ACATTATCAGCAACAACAAATTCAAATGGATTATCCGACATTATTGGCGGCAGCACAGCTTAAATTACAGCAACAGATCA
CGACAACAGCAGAGGTTGAGCGCGTCTTCGGCAAGACGGAGAACAGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-91)

Type II/IV secretion system protein (T2SSE)

(131-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

59.43

97.228

0.578
  pilB Haemophilus influenzae Rd KW20

59.43

97.228

0.578
  pilB Glaesserella parasuis strain SC1401

53.846

99.787

0.537
  pilB Vibrio parahaemolyticus RIMD 2210633

38.677

100

0.412
  pilB Vibrio cholerae strain A1552

39.834

100

0.409
  pilB Vibrio campbellii strain DS40M4

39.627

100

0.407
  pilB Acinetobacter baumannii D1279779

37.832

100

0.394
  pilB Legionella pneumophila strain ERS1305867

36.364

100

0.375
  pilB Acinetobacter baylyi ADP1

39.503

94.456

0.373