Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NYR19_RS10225 Genome accession   NZ_CP103874
Coordinates   2177696..2178157 (-) Length   153 a.a.
NCBI ID   WP_013746605.1    Uniprot ID   F4HD69
Organism   Gallibacterium anatis strain TH22     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2172696..2183157
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR19_RS10195 (NYR19_10205) - 2173206..2173478 (-) 273 WP_018345916.1 GNAT family N-acetyltransferase -
  NYR19_RS10200 (NYR19_10210) yacG 2173562..2173780 (-) 219 WP_018345915.1 DNA gyrase inhibitor YacG -
  NYR19_RS10205 (NYR19_10215) coaE 2173770..2174402 (-) 633 WP_302508097.1 dephospho-CoA kinase -
  NYR19_RS10210 (NYR19_10220) - 2174417..2175049 (-) 633 WP_230586435.1 prepilin peptidase -
  NYR19_RS10215 (NYR19_10225) - 2175046..2176254 (-) 1209 WP_302508101.1 type II secretion system F family protein -
  NYR19_RS10220 (NYR19_10230) pilB 2176270..2177679 (-) 1410 WP_302508102.1 GspE/PulE family protein Machinery gene
  NYR19_RS10225 (NYR19_10235) pilA 2177696..2178157 (-) 462 WP_013746605.1 prepilin peptidase-dependent pilin Machinery gene
  NYR19_RS10230 (NYR19_10240) ampD 2178262..2178816 (+) 555 WP_018345909.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NYR19_RS10235 (NYR19_10245) - 2178903..2179238 (+) 336 WP_013746603.1 hypothetical protein -
  NYR19_RS10240 (NYR19_10250) - 2179347..2180741 (+) 1395 WP_302508103.1 chloride channel protein -
  NYR19_RS10245 (NYR19_10255) dusA 2180722..2181702 (+) 981 WP_302508104.1 tRNA dihydrouridine(20/20a) synthase DusA -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 16390.72 Da        Isoelectric Point: 8.0868

>NTDB_id=726327 NYR19_RS10225 WP_013746605.1 2177696..2178157(-) (pilA) [Gallibacterium anatis strain TH22]
MKKREQLKSNYAGFTLIELMIVIAIIAVLATIAIPSYQNYTKKAAISELIQASSPYKAEVELCIYNTKSKASCNAGQNGI
QAKPSNMDKFKYLKEVNVAAGVISVSGKSSLENISYTMTPSDGNNGELIDWSVTCSGDNGLFPAGFCSNTSAN

Nucleotide


Download         Length: 462 bp        

>NTDB_id=726327 NYR19_RS10225 WP_013746605.1 2177696..2178157(-) (pilA) [Gallibacterium anatis strain TH22]
ATGAAAAAACGTGAACAACTTAAGTCGAATTATGCCGGATTTACTTTAATTGAATTGATGATCGTCATTGCGATTATTGC
CGTGTTGGCAACCATTGCCATTCCCTCTTATCAAAATTACACCAAAAAGGCGGCAATATCAGAATTGATTCAAGCCTCTT
CACCCTATAAGGCGGAAGTTGAACTCTGTATTTATAACACCAAGAGTAAAGCAAGCTGTAACGCCGGTCAAAACGGCATT
CAGGCGAAGCCGTCTAATATGGATAAATTTAAATATCTTAAAGAGGTTAATGTGGCTGCCGGTGTTATTTCTGTTTCCGG
TAAATCCAGTCTGGAGAACATCAGTTATACGATGACACCGAGTGACGGCAATAACGGTGAATTAATTGATTGGAGTGTAA
CTTGTAGCGGTGATAATGGGCTGTTTCCGGCAGGATTTTGTAGCAATACTTCTGCAAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(9-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F4HD69

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

54

98.039

0.529
  pilA Haemophilus influenzae Rd KW20

52.667

98.039

0.516
  pilA Haemophilus influenzae 86-028NP

56.618

88.889

0.503