Detailed information NTG0726100

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: NX081_RS13615
• Genome accession: NZ_CP103856
• Coordinates: 2741112..2741726 (-)
• Length: 204 a.a.
• NCBI ID: WP_060562641.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain SRCM116265
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2736112..2746726

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NX081_RS13585 (NX081_13585)	rpsT	2736411..2736677 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
NX081_RS13590 (NX081_13590)	holA	2736717..2737739 (-)	1023	WP_060562639.1	DNA polymerase III subunit delta	-
NX081_RS13595 (NX081_13595)	-	2737779..2737930 (-)	152	Protein_2636	hypothetical protein	-
NX081_RS13600 (NX081_13600)	-	2737971..2738105 (+)	135	WP_003152870.1	YqzM family protein	-
NX081_RS13605 (NX081_13605)	comEC	2738124..2740475 (-)	2352	WP_060562640.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
NX081_RS13610 (NX081_13610)	-	2740476..2741045 (-)	570	WP_003152868.1	ComE operon protein 2	-
NX081_RS13615 (NX081_13615)	comEA	2741112..2741726 (-)	615	WP_060562641.1	helix-hairpin-helix domain-containing protein	Machinery gene
NX081_RS13620 (NX081_13620)	comER	2741785..2742606 (+)	822	WP_012118027.1	late competence protein ComER	-
NX081_RS13625 (NX081_13625)	-	2742675..2743412 (-)	738	WP_015240247.1	class I SAM-dependent methyltransferase	-
NX081_RS13630 (NX081_13630)	rsfS	2743409..2743765 (-)	357	WP_007408260.1	ribosome silencing factor	-
NX081_RS13635 (NX081_13635)	yqeK	2743783..2744343 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
NX081_RS13640 (NX081_13640)	-	2744333..2744902 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
NX081_RS13645 (NX081_13645)	yhbY	2744913..2745203 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
NX081_RS13650 (NX081_13650)	aroE	2745197..2746039 (-)	843	WP_025285019.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22050.22 Da
• Isoelectric Point: 6.4803

>NTDB_id=726100 NX081_RS13615 WP_060562641.1 2741112..2741726(-) (comEA) [Bacillus velezensis strain SRCM116265]
MTKAMMTERLLRNLKILGACLAAGAVFICIWLFRSDKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLGELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=726100 NX081_RS13615 WP_060562641.1 2741112..2741726(-) (comEA) [Bacillus velezensis strain SRCM116265]
ATGACGAAAGCGATGATGACGGAGCGGCTGCTGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGACAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTATAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGGCGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae R6	35.377	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.377	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.377	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.377	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.377	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	34.906	100	0.363