Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR80_RS04820 Genome accession   NZ_CP103825
Coordinates   1078732..1080117 (-) Length   461 a.a.
NCBI ID   WP_279439096.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 3282     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1073732..1085117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR80_RS04790 (NYR80_04795) lptE 1074338..1074832 (+) 495 WP_278225775.1 LPS assembly lipoprotein LptE -
  NYR80_RS04795 (NYR80_04800) holA 1074940..1075965 (+) 1026 WP_279439092.1 DNA polymerase III subunit delta -
  NYR80_RS04800 (NYR80_04805) yacG 1076042..1076230 (-) 189 WP_115589824.1 DNA gyrase inhibitor YacG -
  NYR80_RS04805 (NYR80_04810) coaE 1076212..1076850 (-) 639 WP_279439093.1 dephospho-CoA kinase -
  NYR80_RS04810 (NYR80_04815) - 1076872..1077546 (-) 675 WP_279439094.1 prepilin peptidase -
  NYR80_RS04815 (NYR80_04820) pilC 1077546..1078739 (-) 1194 WP_279439095.1 type II secretion system F family protein Machinery gene
  NYR80_RS04820 (NYR80_04825) pilB 1078732..1080117 (-) 1386 WP_279439096.1 GspE/PulE family protein Machinery gene
  NYR80_RS04825 (NYR80_04830) pilA 1080138..1080575 (-) 438 WP_279439097.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR80_RS04830 (NYR80_04835) radA 1080756..1082135 (+) 1380 WP_279439098.1 DNA repair protein RadA Machinery gene
  NYR80_RS04835 (NYR80_04840) pepB 1082201..1083484 (+) 1284 WP_279439187.1 aminopeptidase PepB -
  NYR80_RS04840 (NYR80_04845) - 1083612..1084547 (+) 936 WP_279439099.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR80_RS04845 (NYR80_04850) greB 1084557..1085045 (+) 489 WP_005611555.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52419.94 Da        Isoelectric Point: 5.1201

>NTDB_id=725620 NYR80_RS04820 WP_279439096.1 1078732..1080117(-) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 3282]
MQYSVCDMNSQQLFEISDSLWQKNCDEKHVLLRYLAMPIQENDDTLWLAIDDMNNLSACEIFAFIAHKTIEPVLISSDEL
KYLLNQLSPEQQSVYEETELNYQSPDNLELLNQNDPIIQILDNLFKFCLKQNASDIHFEPQKEKLLIRLRIDGVLHIYKS
LSFSLANRIITRLKLLAKLDISETRLPQDGQFNFKTVTAETLDFRVSSLPTNFGEKIVLRLQKNKPTHFDFLELGFSPEQ
KQLLLTALHQPQGLILVTGPTGSGKSITLYSALNYLNNSERHILTAEDPIEIEIEGIIQTQVNRAIQLDFSQLLRTFLRQ
DPDIIMLGEIRDQESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGVKEYEIKNSLQLIIAQRLLRKSCLQCGGKGC
DSCYHGYKGRTGIYQLLAKQGNFFEKSTAYLDYDNLRASAEQKLAANITTVDEVKRVLGDG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=725620 NYR80_RS04820 WP_279439096.1 1078732..1080117(-) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 3282]
ATGCAGTATTCGGTTTGTGATATGAACAGCCAGCAGCTATTTGAAATTTCAGACTCGTTGTGGCAAAAAAATTGTGATGA
AAAACATGTTTTATTACGTTATTTGGCTATGCCGATTCAAGAAAATGACGATACGCTATGGCTTGCAATTGATGATATGA
ATAATTTGAGTGCTTGTGAAATTTTTGCATTTATTGCGCATAAAACAATTGAACCTGTTTTGATTTCCTCCGATGAATTG
AAGTATTTACTTAATCAACTTTCGCCAGAACAACAATCCGTATATGAAGAAACCGAGTTAAATTATCAATCGCCGGATAA
TTTAGAACTACTCAATCAAAATGACCCCATTATTCAGATTTTAGATAATTTATTTAAATTTTGCTTAAAGCAAAATGCTT
CGGATATTCATTTTGAGCCACAAAAAGAGAAGTTACTGATTAGATTACGTATTGATGGGGTATTGCATATCTATAAAAGC
TTATCGTTTAGTTTAGCCAATCGTATTATCACAAGGCTTAAACTCTTAGCTAAATTGGATATTAGTGAGACACGTTTACC
ACAAGATGGGCAGTTTAATTTTAAGACAGTAACGGCCGAAACATTAGATTTTCGAGTATCCAGCTTACCAACTAACTTTG
GTGAGAAAATCGTCTTGCGTTTACAAAAGAATAAGCCTACTCATTTCGATTTTTTAGAATTAGGTTTTTCGCCGGAACAA
AAGCAATTATTACTTACGGCGTTACACCAGCCACAAGGGTTGATTCTGGTTACGGGGCCAACAGGCAGTGGTAAAAGTAT
TACTTTATATAGTGCGTTAAATTATTTAAATAACAGCGAACGGCATATTTTAACCGCAGAAGATCCGATTGAAATTGAGA
TAGAAGGGATTATCCAAACTCAAGTTAATCGCGCTATTCAATTGGATTTTAGTCAATTGCTCAGAACATTTTTACGTCAG
GATCCGGACATTATTATGTTAGGTGAAATCCGTGATCAGGAAAGTGCTGAAATTGCATTAAGAGCAGCGCAAACAGGGCA
TCTAGTGCTTTCTACTTTACATACAAATGATGCGCCGTCTGCGATTGAACGCTTATTACAATTGGGAGTTAAAGAGTACG
AGATTAAGAATTCTCTACAACTGATCATTGCACAGCGTTTATTACGTAAAAGTTGTCTTCAATGTGGCGGAAAAGGGTGT
GATAGCTGTTATCACGGTTATAAAGGGCGAACGGGAATTTACCAATTACTTGCGAAACAAGGTAATTTTTTTGAAAAATC
GACCGCTTATTTGGATTATGACAACCTACGAGCCAGTGCCGAACAAAAGTTAGCGGCTAACATAACAACAGTAGACGAAG
TAAAGCGGGTGTTAGGTGATGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.052

99.349

0.716
  pilB Haemophilus influenzae Rd KW20

57.428

97.831

0.562
  pilB Haemophilus influenzae 86-028NP

56.763

97.831

0.555
  pilB Vibrio parahaemolyticus RIMD 2210633

40.333

100

0.421
  pilB Vibrio campbellii strain DS40M4

40.125

100

0.419
  pilB Vibrio cholerae strain A1552

39.834

100

0.416
  pilB Legionella pneumophila strain ERS1305867

39.024

100

0.416
  pilB Acinetobacter baylyi ADP1

38.431

100

0.414
  pilB Acinetobacter baumannii D1279779

39.3

100

0.414