Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NYR80_RS04825 Genome accession   NZ_CP103825
Coordinates   1080138..1080575 (-) Length   145 a.a.
NCBI ID   WP_279439097.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 3282     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1075138..1085575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR80_RS04800 (NYR80_04805) yacG 1076042..1076230 (-) 189 WP_115589824.1 DNA gyrase inhibitor YacG -
  NYR80_RS04805 (NYR80_04810) coaE 1076212..1076850 (-) 639 WP_279439093.1 dephospho-CoA kinase -
  NYR80_RS04810 (NYR80_04815) - 1076872..1077546 (-) 675 WP_279439094.1 prepilin peptidase -
  NYR80_RS04815 (NYR80_04820) pilC 1077546..1078739 (-) 1194 WP_279439095.1 type II secretion system F family protein Machinery gene
  NYR80_RS04820 (NYR80_04825) pilB 1078732..1080117 (-) 1386 WP_279439096.1 GspE/PulE family protein Machinery gene
  NYR80_RS04825 (NYR80_04830) pilA 1080138..1080575 (-) 438 WP_279439097.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR80_RS04830 (NYR80_04835) radA 1080756..1082135 (+) 1380 WP_279439098.1 DNA repair protein RadA Machinery gene
  NYR80_RS04835 (NYR80_04840) pepB 1082201..1083484 (+) 1284 WP_279439187.1 aminopeptidase PepB -
  NYR80_RS04840 (NYR80_04845) - 1083612..1084547 (+) 936 WP_279439099.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR80_RS04845 (NYR80_04850) greB 1084557..1085045 (+) 489 WP_005611555.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15499.86 Da        Isoelectric Point: 8.0828

>NTDB_id=725621 NYR80_RS04825 WP_279439097.1 1080138..1080575(-) (pilA) [Actinobacillus equuli subsp. haemolyticus strain 3282]
MKFVFKPIQQAFTLIELMIVIAIIAILATVAIPSYNNYTKKAALSELLAASSAYKTDVEICIYNTGEVKNCSSGTNGIKN
GNDIKNSKYLASISVASGVITVKGKGSVDGYGYTLTPDYSNKTLTWETACLGEDTSLFPAAFCSK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=725621 NYR80_RS04825 WP_279439097.1 1080138..1080575(-) (pilA) [Actinobacillus equuli subsp. haemolyticus strain 3282]
ATGAAATTCGTATTCAAGCCTATACAGCAGGCATTTACCCTGATTGAATTAATGATTGTAATTGCAATCATTGCTATTTT
GGCAACGGTAGCCATTCCTTCTTATAACAATTACACCAAAAAGGCGGCATTATCCGAACTGTTAGCGGCATCTTCCGCTT
ATAAAACAGATGTGGAAATTTGTATTTATAACACGGGAGAGGTAAAAAACTGTTCTAGCGGAACGAATGGCATCAAAAAT
GGTAACGATATCAAAAACTCTAAGTATTTAGCCTCTATTTCTGTAGCTTCCGGTGTAATAACTGTGAAGGGGAAAGGGAG
TGTAGATGGGTATGGATATACGCTTACACCGGATTATTCAAATAAAACGCTAACTTGGGAAACGGCTTGTTTAGGCGAGG
ATACTAGTCTATTCCCTGCCGCGTTTTGTTCGAAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-128)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

65.517

100

0.655
  pilA Haemophilus influenzae Rd KW20

56.738

97.241

0.552
  pilA Haemophilus influenzae 86-028NP

56.429

96.552

0.545
  pilA Vibrio campbellii strain DS40M4

37.589

97.241

0.366