Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PZ484_RS22775 Genome accession   NZ_CP119298
Coordinates   4891037..4892446 (-) Length   469 a.a.
NCBI ID   WP_213870487.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SNDPR-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4886037..4897446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PZ484_RS22765 - 4888131..4889309 (-) 1179 WP_003085359.1 substrate-binding domain-containing protein -
  PZ484_RS22770 pilC 4889823..4891037 (-) 1215 WP_022579875.1 type II secretion system inner membrane protein GspF Machinery gene
  PZ484_RS22775 pilB 4891037..4892446 (-) 1410 WP_213870487.1 type II secretion system ATPase GspE Machinery gene
  PZ484_RS22780 gspD 4892443..4894854 (-) 2412 WP_213870489.1 type II secretion system secretin GspD -
  PZ484_RS22785 gspM 4894851..4895444 (-) 594 WP_010793838.1 type II secretion system protein GspM -
  PZ484_RS22790 gspL 4895431..4896642 (-) 1212 WP_016253830.1 type II secretion system protein GspL -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 50948.34 Da        Isoelectric Point: 5.7943

>NTDB_id=725616 PZ484_RS22775 WP_213870487.1 4891037..4892446(-) (pilB) [Pseudomonas aeruginosa strain SNDPR-01]
MSLLPYAWAKAQRALLRPGEHGATLLVSPRTPGWAISEVRQRHAPASLESVRDDELDTLLASAYSDTGSAAAVVGAAESE
VDLDRLMDDIPEVTDLLDTQDGAPVIRMINALLTQAARDEASDIHIEPFETHSVVRYRVDGALRDVVAPRKALHAALVSR
IKIMAQLDIAEKRLPQDGRIALRVAGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLRLETLGMAPGVLAPLDNLIRQPHG
IVLVTGPTGSGKTTTLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNARIDMTFAVALRAILRQDPDIIMIGEIRDL
ETAQIAVQASLTGHLVLATLHTNDAVSAVTRLVDMGVEPFLLASSMLGVLAQRLVRRLCTHCRVEEDGGWRAVGCPACNQ
TGYSGRTGIHELFVIDDEIRRLVHQGRAEQDLREAARAAGMRSMREDGERWIASGSATLEEILRVTRDA

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=725616 PZ484_RS22775 WP_213870487.1 4891037..4892446(-) (pilB) [Pseudomonas aeruginosa strain SNDPR-01]
ATGAGCCTGCTGCCCTACGCCTGGGCCAAGGCGCAGCGCGCCTTGCTGCGGCCGGGCGAGCATGGCGCGACCCTGCTGGT
CAGCCCGCGCACGCCCGGCTGGGCGATCAGCGAGGTGCGCCAGCGGCATGCGCCGGCCAGCCTGGAGAGCGTCCGCGACG
ACGAGCTCGACACCCTGCTGGCCAGCGCCTACTCCGATACCGGCAGCGCCGCGGCGGTGGTCGGCGCGGCGGAGAGCGAG
GTCGACCTCGACCGCCTGATGGACGATATCCCCGAGGTTACCGACCTGCTCGACACCCAGGATGGCGCGCCAGTGATCCG
CATGATCAACGCGCTGCTGACCCAGGCCGCGCGCGATGAGGCCAGCGACATCCACATCGAGCCCTTCGAGACCCATTCGG
TGGTGCGCTACCGCGTCGACGGCGCCCTGCGCGACGTGGTCGCGCCGCGCAAGGCGCTGCACGCGGCGCTGGTGTCGCGG
ATCAAGATCATGGCGCAGCTGGATATCGCTGAGAAACGCCTGCCCCAGGACGGCCGCATCGCCTTGCGGGTAGCCGGGCG
GCCGATCGACATTCGCGTCTCCACCGTGCCCACCGGCCACGGCGAACGGGTGGTGATGCGCCTGCTGGACAAGCAGGCCG
GACGCCTGAGGCTGGAGACCCTGGGCATGGCTCCCGGCGTGCTGGCGCCGCTGGACAACCTGATCCGCCAGCCGCACGGC
ATCGTGCTGGTCACCGGGCCCACCGGCAGCGGCAAGACCACCACCCTCTACGCCGCCCTGGCGCGGCTCGACGCCAGCAC
CAGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGACCTGCCGGGGATCAGCCAGATCCAGGTCAACGCGCGCATCG
ACATGACCTTCGCCGTAGCCCTGCGGGCGATCCTCCGGCAGGACCCGGACATCATCATGATCGGCGAGATCCGCGACCTG
GAGACCGCGCAGATCGCCGTGCAGGCCTCGCTCACCGGGCACCTGGTGCTGGCCACGCTGCACACCAACGACGCGGTGTC
GGCAGTGACGCGGCTGGTGGACATGGGCGTCGAGCCGTTCCTGCTCGCCTCGTCGATGCTTGGCGTACTCGCCCAGCGCC
TGGTGCGGCGCCTGTGCACGCATTGCCGGGTCGAAGAGGACGGCGGCTGGCGCGCGGTCGGCTGCCCGGCTTGCAACCAG
ACCGGCTACAGCGGGCGCACCGGCATCCACGAACTGTTCGTGATCGACGACGAGATCCGCCGCCTGGTCCACCAGGGCCG
CGCCGAGCAGGATCTCCGCGAAGCCGCCCGCGCCGCCGGTATGCGCAGCATGCGCGAGGACGGCGAGCGCTGGATCGCCA
GCGGCTCCGCCACCCTGGAGGAAATCCTCCGCGTGACCCGGGACGCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(111-377)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

45.283

90.405

0.409
  pilF Thermus thermophilus HB27

50.663

80.384

0.407
  pilB/pilB1 Synechocystis sp. PCC 6803

40.899

99.574

0.407
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.205

83.156

0.401
  pilF Neisseria gonorrhoeae MS11

46.954

84.009

0.394
  pilB Vibrio campbellii strain DS40M4

46.076

84.222

0.388
  pilB Acinetobacter baumannii D1279779

41.176

94.243

0.388
  pilB Vibrio parahaemolyticus RIMD 2210633

45.823

84.222

0.386
  pilB Legionella pneumophila strain ERS1305867

45.244

82.942

0.375
  pilB Acinetobacter baylyi ADP1

44.845

82.729

0.371