Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR74_RS00380 Genome accession   NZ_CP103819
Coordinates   85740..87125 (+) Length   461 a.a.
NCBI ID   WP_279454274.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 3873     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 80740..92125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR74_RS00355 (NYR74_00355) greB 80813..81301 (-) 489 WP_005611555.1 transcription elongation factor GreB -
  NYR74_RS00360 (NYR74_00360) - 81311..82246 (-) 936 WP_278225782.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR74_RS00365 (NYR74_00365) pepB 82374..83657 (-) 1284 WP_279454091.1 aminopeptidase PepB -
  NYR74_RS00370 (NYR74_00370) radA 83723..85102 (-) 1380 WP_279443725.1 DNA repair protein RadA Machinery gene
  NYR74_RS00375 (NYR74_00375) pilA 85282..85719 (+) 438 WP_005623151.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR74_RS00380 (NYR74_00380) pilB 85740..87125 (+) 1386 WP_279454274.1 GspE/PulE family protein Machinery gene
  NYR74_RS00385 (NYR74_00385) - 87118..88311 (+) 1194 WP_279454032.1 type II secretion system F family protein -
  NYR74_RS00390 (NYR74_00390) - 88311..88787 (+) 477 WP_279454031.1 hypothetical protein -
  NYR74_RS00395 (NYR74_00395) - 88834..88986 (+) 153 WP_279454030.1 hypothetical protein -
  NYR74_RS00400 (NYR74_00400) coaE 89008..89646 (+) 639 WP_279454029.1 dephospho-CoA kinase -
  NYR74_RS00405 (NYR74_00405) yacG 89628..89816 (+) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  NYR74_RS00410 (NYR74_00410) holA 89893..90918 (-) 1026 WP_279454028.1 DNA polymerase III subunit delta -
  NYR74_RS00415 (NYR74_00415) - 90875..91021 (+) 147 WP_278225056.1 hypothetical protein -
  NYR74_RS00420 (NYR74_00420) lptE 91026..91520 (-) 495 WP_278225775.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52484.06 Da        Isoelectric Point: 5.3508

>NTDB_id=725483 NYR74_RS00380 WP_279454274.1 85740..87125(+) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 3873]
MQYSVCDMNSQQVFDISESLWQKNCDEKHILLRYLAIPIQENDDTLWLAIDDMNNLSACEIFAFITHKTIEPVLISSDEL
KYLLNQLSPEQQSLYEETELNYQSPDNLELLNQNDPIIQILDNLFKFCLKQNASDIHFEPQKEKLLIRLRIDGVLHIYKS
LSFSLANRIITRLKLLAKLDISETRLPQDGQFNFKTVTAETLDFRVSSLPTSFGEKIVLRLQKNKPTHFDFLELGFSPEQ
KQLLLTALHQPQGLIPVTGPTGSGKSITLYSALNYLNNSERHILTAEDPIEIEIEGIIQTQVNRAIQLDFSQLLRTFLRQ
DPDIIMLGEIRDQESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEIKNSLQLIIAQRLLRKRCPQCGGKGC
DNCYHGYKGRTGIYQLLAKQGKFFEKSTAYLDYDSLRASAEQKLAANITTVDEVKRVLGDG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=725483 NYR74_RS00380 WP_279454274.1 85740..87125(+) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 3873]
ATGCAGTATTCGGTTTGTGATATGAATAGCCAGCAGGTATTTGATATCTCTGAATCCTTGTGGCAGAAAAATTGTGACGA
AAAACATATTTTGTTGCGTTATTTAGCCATTCCGATTCAAGAAAATGACGATACGTTATGGCTTGCAATTGATGATATGA
ATAATTTAAGTGCTTGTGAAATTTTTGCATTTATTACGCATAAAACAATTGAACCTGTTTTGATTTCCTCCGATGAATTG
AAGTACTTACTTAATCAACTTTCGCCGGAACAACAATCTTTATATGAAGAAACCGAGTTAAATTATCAATCGCCAGATAA
TTTAGAACTACTCAATCAAAATGATCCTATTATTCAGATTTTAGATAATTTATTTAAATTTTGCTTAAAGCAAAATGCTT
CGGATATTCATTTTGAGCCACAAAAAGAGAAGTTACTGATTAGATTACGTATTGATGGGGTATTGCACATCTATAAAAGT
TTATCGTTTAGTTTAGCTAATCGTATTATCACGAGGCTTAAATTGTTAGCTAAATTGGATATTAGTGAGACACGCTTACC
ACAAGACGGGCAGTTTAATTTTAAGACGGTAACAGCTGAGACATTAGATTTTCGAGTGTCCAGTTTACCGACTAGCTTCG
GTGAGAAAATAGTCTTGCGCTTGCAAAAGAATAAGCCTACCCATTTTGATTTTTTGGAATTAGGTTTTTCGCCGGAACAA
AAGCAATTATTACTTACAGCGTTACACCAGCCACAAGGGTTGATTCCGGTTACGGGACCAACAGGCAGTGGCAAAAGTAT
TACTTTATATAGTGCGTTAAATTATTTAAATAATAGCGAACGGCATATTCTGACTGCAGAAGACCCGATTGAAATTGAGA
TAGAAGGGATTATCCAAACTCAAGTAAATCGTGCAATTCAATTAGACTTTAGCCAATTACTCAGAACATTTTTACGTCAG
GATCCGGACATTATTATGTTAGGTGAAATCCGTGATCAGGAAAGTGCTGAAATCGCATTAAGAGCAGCGCAAACAGGACA
TCTTGTACTTTCTACTTTACATACGAATGATGCGCCGTCTGCGATTGAGCGTTTACTACAATTAGGGATTAAAGAGTATG
AGATTAAGAATTCGCTGCAACTGATTATCGCACAGCGTTTATTACGTAAACGTTGTCCGCAATGTGGCGGAAAAGGGTGT
GATAACTGTTATCACGGTTATAAAGGGCGAACAGGAATTTACCAATTGCTTGCCAAGCAAGGTAAATTTTTTGAAAAATC
AACCGCTTATTTGGATTATGACAGCTTACGAGCCAGTGCCGAACAAAAGTTAGCGGCTAACATAACAACAGTAGACGAAG
TAAAGCGGGTGTTAGGTGATGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

72.926

99.349

0.725
  pilB Haemophilus influenzae Rd KW20

57.206

97.831

0.56
  pilB Haemophilus influenzae 86-028NP

56.541

97.831

0.553
  pilB Vibrio parahaemolyticus RIMD 2210633

40.541

100

0.423
  pilB Legionella pneumophila strain ERS1305867

39.634

100

0.423
  pilB Vibrio campbellii strain DS40M4

40.206

100

0.423
  pilB Vibrio cholerae strain A1552

38.956

100

0.421
  pilB Acinetobacter baumannii D1279779

38.306

100

0.412
  pilB Acinetobacter baylyi ADP1

37.575

100

0.41