Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NYR74_RS00375 Genome accession   NZ_CP103819
Coordinates   85282..85719 (+) Length   145 a.a.
NCBI ID   WP_005623151.1    Uniprot ID   E8KHR2
Organism   Actinobacillus equuli subsp. haemolyticus strain 3873     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 80282..90719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR74_RS00355 (NYR74_00355) greB 80813..81301 (-) 489 WP_005611555.1 transcription elongation factor GreB -
  NYR74_RS00360 (NYR74_00360) - 81311..82246 (-) 936 WP_278225782.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR74_RS00365 (NYR74_00365) pepB 82374..83657 (-) 1284 WP_279454091.1 aminopeptidase PepB -
  NYR74_RS00370 (NYR74_00370) radA 83723..85102 (-) 1380 WP_279443725.1 DNA repair protein RadA Machinery gene
  NYR74_RS00375 (NYR74_00375) pilA 85282..85719 (+) 438 WP_005623151.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR74_RS00380 (NYR74_00380) pilB 85740..87125 (+) 1386 WP_279454274.1 GspE/PulE family protein Machinery gene
  NYR74_RS00385 (NYR74_00385) - 87118..88311 (+) 1194 WP_279454032.1 type II secretion system F family protein -
  NYR74_RS00390 (NYR74_00390) - 88311..88787 (+) 477 WP_279454031.1 hypothetical protein -
  NYR74_RS00395 (NYR74_00395) - 88834..88986 (+) 153 WP_279454030.1 hypothetical protein -
  NYR74_RS00400 (NYR74_00400) coaE 89008..89646 (+) 639 WP_279454029.1 dephospho-CoA kinase -
  NYR74_RS00405 (NYR74_00405) yacG 89628..89816 (+) 189 WP_005623139.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15335.53 Da        Isoelectric Point: 7.6225

>NTDB_id=725482 NYR74_RS00375 WP_005623151.1 85282..85719(+) (pilA) [Actinobacillus equuli subsp. haemolyticus strain 3873]
MKHIIKPIQQAFTLIELMIVIAIIAILATVAIPSYNNYTKKAALSELLAASSAYKTDVEICIYNTGATNSCSSGANGIKN
GDDLKNSKYLDSVSVTAGTITVNGKGSVAGYGYTLTPDYSSKTLTWKTTCSGEDKSLFPAAFCSE

Nucleotide


Download         Length: 438 bp        

>NTDB_id=725482 NYR74_RS00375 WP_005623151.1 85282..85719(+) (pilA) [Actinobacillus equuli subsp. haemolyticus strain 3873]
ATGAAACACATAATTAAACCGATACAACAGGCATTTACCCTGATTGAATTAATGATTGTGATTGCAATCATTGCAATTTT
AGCAACTGTGGCGATTCCTTCTTATAACAATTACACCAAAAAAGCGGCATTATCCGAACTGTTAGCGGCATCTTCTGCAT
ATAAAACGGATGTAGAAATTTGTATTTATAACACTGGAGCTACCAATAGCTGTTCTAGTGGAGCAAATGGCATTAAGAAT
GGCGATGATCTTAAAAATTCTAAGTATTTAGATTCAGTCTCTGTAACTGCGGGTACTATAACCGTGAATGGCAAGGGCAG
CGTAGCGGGATATGGTTATACACTTACACCAGATTATTCAAGTAAAACACTGACGTGGAAAACAACCTGTTCAGGAGAGG
ATAAAAGTTTATTCCCCGCAGCATTTTGCTCGGAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-139)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E8KHR2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

60.959

100

0.614
  pilA Haemophilus influenzae Rd KW20

56.738

97.241

0.552
  pilA Haemophilus influenzae 86-028NP

56.429

96.552

0.545
  comP Acinetobacter baylyi ADP1

41.844

97.241

0.407