Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYR73_RS05050 Genome accession   NZ_CP103818
Coordinates   1110592..1111977 (-) Length   461 a.a.
NCBI ID   WP_279454033.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 3874     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1105592..1116977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR73_RS05015 (NYR73_05015) lptE 1106197..1106691 (+) 495 WP_278225775.1 LPS assembly lipoprotein LptE -
  NYR73_RS05020 (NYR73_05020) holA 1106799..1107824 (+) 1026 WP_279454028.1 DNA polymerase III subunit delta -
  NYR73_RS05025 (NYR73_05025) yacG 1107901..1108089 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  NYR73_RS05030 (NYR73_05030) coaE 1108071..1108709 (-) 639 WP_279454029.1 dephospho-CoA kinase -
  NYR73_RS05035 (NYR73_05035) - 1108731..1108883 (-) 153 WP_279454030.1 hypothetical protein -
  NYR73_RS05040 (NYR73_05040) - 1108930..1109406 (-) 477 WP_279454031.1 hypothetical protein -
  NYR73_RS05045 (NYR73_05045) - 1109406..1110599 (-) 1194 WP_279454032.1 type II secretion system F family protein -
  NYR73_RS05050 (NYR73_05050) pilB 1110592..1111977 (-) 1386 WP_279454033.1 GspE/PulE family protein Machinery gene
  NYR73_RS05055 (NYR73_05055) pilA 1111998..1112435 (-) 438 WP_005623151.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR73_RS05060 (NYR73_05060) radA 1112615..1113994 (+) 1380 WP_279443725.1 DNA repair protein RadA Machinery gene
  NYR73_RS05065 (NYR73_05065) pepB 1114060..1115343 (+) 1284 WP_279454091.1 aminopeptidase PepB -
  NYR73_RS05070 (NYR73_05070) - 1115471..1116406 (+) 936 WP_278225782.1 KpsF/GutQ family sugar-phosphate isomerase -
  NYR73_RS05075 (NYR73_05075) greB 1116416..1116904 (+) 489 WP_005611555.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52500.10 Da        Isoelectric Point: 5.3508

>NTDB_id=725465 NYR73_RS05050 WP_279454033.1 1110592..1111977(-) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 3874]
MQYSVCDMNSQQVFDISESLWQKNCDEKHILLRYLAIPIQENDDTLWLAIDDMNNLSACEIFAFITHKTIEPVLISSDEL
KYLLNQLSPEQQSLYEETELNYQSPDNLELLNQNDPIIQILDNLFKFCLKQNASDIHFEPQKEKLLIRLRIDGVLHIYKS
LSFSLANRIITRLKLLAKLDISETRLPQDGQFNFKTVTAETLDFRVSSLPTSFGEKIVLRLQKNKPTHFDFLELGFSPEQ
KQLLLTALHQPQGLILVTGPTGSGKSITLYSALNYLNNSERHILTAEDPIEIEIEGIIQTQVNRAIQLDFSQLLRTFLRQ
DPDIIMLGEIRDQESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEIKNSLQLIIAQRLLRKRCPQCGGKGC
DNCYHGYKGRTGIYQLLAKQGKFFEKSTAYLDYDSLRASAEQKLAANITTVDEVKRVLGDG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=725465 NYR73_RS05050 WP_279454033.1 1110592..1111977(-) (pilB) [Actinobacillus equuli subsp. haemolyticus strain 3874]
ATGCAGTATTCGGTTTGTGATATGAATAGCCAGCAGGTATTTGATATCTCTGAATCCTTGTGGCAGAAAAATTGTGACGA
AAAACATATTTTGTTGCGTTATTTAGCCATTCCGATTCAAGAAAATGACGATACGTTATGGCTTGCAATTGATGATATGA
ATAATTTAAGTGCTTGTGAAATTTTTGCATTTATTACGCATAAAACAATTGAACCTGTTTTGATTTCCTCCGATGAATTG
AAGTACTTACTTAATCAACTTTCGCCGGAACAACAATCTTTATATGAAGAAACCGAGTTAAATTATCAATCGCCAGATAA
TTTAGAACTACTCAATCAAAATGATCCTATTATTCAGATTTTAGATAATTTATTTAAATTTTGCTTAAAGCAAAATGCTT
CGGATATTCATTTTGAGCCACAAAAAGAGAAGTTACTGATTAGATTACGTATTGATGGGGTATTGCACATCTATAAAAGT
TTATCGTTTAGTTTAGCTAATCGTATTATCACGAGGCTTAAATTGTTAGCTAAATTGGATATTAGTGAGACACGCTTACC
ACAAGACGGGCAGTTTAATTTTAAGACGGTAACAGCTGAGACATTAGATTTTCGAGTGTCCAGTTTACCGACTAGCTTCG
GTGAGAAAATAGTCTTGCGCTTGCAAAAGAATAAGCCTACCCATTTTGATTTTTTGGAATTAGGTTTTTCGCCGGAACAA
AAGCAATTATTACTTACAGCGTTACACCAGCCACAAGGGTTGATTCTGGTTACGGGACCAACAGGCAGTGGCAAAAGTAT
TACTTTATATAGTGCGTTAAATTATTTAAATAATAGCGAACGGCATATTCTGACTGCAGAAGACCCGATTGAAATTGAGA
TAGAAGGGATTATCCAAACTCAAGTAAATCGTGCAATTCAATTAGACTTTAGCCAATTACTCAGAACATTTTTACGTCAG
GATCCGGACATTATTATGTTAGGTGAAATCCGTGATCAGGAAAGTGCTGAAATCGCATTAAGAGCAGCGCAAACAGGACA
TCTTGTACTTTCTACTTTACATACGAATGATGCGCCGTCTGCGATTGAGCGTTTACTACAATTAGGGATTAAAGAGTATG
AGATTAAGAATTCGCTGCAACTGATTATCGCACAGCGTTTATTACGTAAACGTTGTCCGCAATGTGGCGGAAAAGGGTGT
GATAACTGTTATCACGGTTATAAAGGGCGAACAGGAATTTACCAATTGCTTGCCAAGCAAGGTAAATTTTTTGAAAAATC
AACCGCTTATTTGGATTATGACAGCTTACGAGCCAGTGCCGAACAAAAGTTAGCGGCTAACATAACAACAGTAGACGAAG
TAAAGCGGGTGTTAGGTGATGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

73.144

99.349

0.727
  pilB Haemophilus influenzae Rd KW20

57.428

97.831

0.562
  pilB Haemophilus influenzae 86-028NP

56.763

97.831

0.555
  pilB Vibrio campbellii strain DS40M4

40.412

100

0.425
  pilB Legionella pneumophila strain ERS1305867

39.837

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

40.748

100

0.425
  pilB Vibrio cholerae strain A1552

39.157

100

0.423
  pilB Acinetobacter baumannii D1279779

38.508

100

0.414
  pilB Acinetobacter baylyi ADP1

37.773

100

0.412