Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PUL49_RS24350 Genome accession   NZ_CP118641
Coordinates   5229628..5231037 (-) Length   469 a.a.
NCBI ID   WP_281004910.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain P23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5224628..5236037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUL49_RS24340 (PUL49_24325) - 5226722..5227900 (-) 1179 WP_023116094.1 substrate-binding domain-containing protein -
  PUL49_RS24345 (PUL49_24330) pilC 5228414..5229628 (-) 1215 WP_003141142.1 type II secretion system inner membrane protein GspF Machinery gene
  PUL49_RS24350 (PUL49_24335) pilB 5229628..5231037 (-) 1410 WP_281004910.1 type II secretion system ATPase GspE Machinery gene
  PUL49_RS24355 (PUL49_24340) gspD 5231034..5233445 (-) 2412 WP_009315212.1 type II secretion system secretin GspD -
  PUL49_RS24360 (PUL49_24345) gspM 5233442..5234035 (-) 594 WP_003112724.1 type II secretion system protein GspM -
  PUL49_RS24365 (PUL49_24350) gspL 5234022..5235233 (-) 1212 WP_043096066.1 type II secretion system protein GspL -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 50936.28 Da        Isoelectric Point: 5.7943

>NTDB_id=723311 PUL49_RS24350 WP_281004910.1 5229628..5231037(-) (pilB) [Pseudomonas aeruginosa strain P23]
MSLLPYAWAKAQRALLRPGEHGATLLVSPRTPGWAISEVRQRHAPASLESVRDDELDTLLASAYSDTGSAAAVVGAAESE
VDLDRLMDDIPEVTDLLDTQDGAPVIRMINALLTQAARDEASDIHIEPFETHSVVRYRVDGALRDVVAPRKALHAALVSR
IKIMAQLDIAEKRLPQDGRIALRGAGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLRLETLGMAPGVLAPLDNLIRQPHG
IVLVTGPTGSGKTTTLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNARIDMTFAVALRAILRQDPDIIMIGEIRDL
ETAQIAVQASLTGHLVLATLHTNDAVSAVTRLVDMGVEPFLLASSMLGVLAQRLVRRLCTHCRVEEDGGWRAVGCPACNQ
TGYSGRTGIHELFVIDDEIRRLVHQGRAEQDLREAARAAGMRSMREDGERWIASGSTTLEEILRVTRDA

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=723311 PUL49_RS24350 WP_281004910.1 5229628..5231037(-) (pilB) [Pseudomonas aeruginosa strain P23]
ATGAGCCTGCTGCCCTACGCCTGGGCCAAGGCGCAGCGCGCCTTGCTGCGGCCGGGCGAGCATGGCGCGACCCTGCTGGT
CAGCCCGCGGACGCCCGGCTGGGCGATCAGCGAGGTGCGCCAGCGGCATGCGCCGGCCAGCCTGGAGAGCGTCCGCGACG
ACGAGCTCGACACCCTGCTGGCCAGCGCCTACTCCGATACCGGCAGCGCCGCGGCGGTGGTCGGCGCGGCGGAGAGCGAG
GTCGACCTCGACCGCCTGATGGACGATATCCCCGAGGTTACCGACCTGCTCGACACCCAGGATGGCGCGCCAGTGATCCG
CATGATCAACGCGCTGCTGACCCAGGCCGCGCGCGATGAGGCCAGCGACATACACATCGAGCCCTTCGAGACCCATTCGG
TGGTGCGCTACCGGGTCGACGGCGCCCTGCGCGACGTGGTCGCGCCGCGCAAGGCGCTGCACGCGGCGCTGGTGTCGCGG
ATCAAGATCATGGCGCAGCTGGATATCGCCGAGAAACGCCTGCCCCAGGACGGCCGCATCGCCTTGCGGGGCGCCGGGCG
GCCGATCGACATTCGCGTCTCCACCGTGCCCACCGGCCACGGCGAACGGGTGGTGATGCGCCTGCTGGACAAGCAGGCCG
GACGCCTGAGGCTGGAGACCCTGGGCATGGCTCCCGGCGTGCTGGCGCCGCTGGACAACCTGATCCGCCAGCCGCACGGC
ATCGTGCTGGTCACCGGGCCCACCGGCAGCGGCAAGACCACCACCCTCTACGCCGCCCTGGCGCGGCTCGACGCCAGCAC
CAGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGACCTGCCGGGGATCAGCCAGATCCAGGTCAACGCGCGCATCG
ACATGACCTTCGCCGTAGCCCTGCGGGCGATCCTCCGGCAGGACCCGGACATCATCATGATCGGCGAGATCCGCGACCTG
GAGACCGCGCAGATCGCCGTGCAGGCTTCGCTCACCGGGCACCTGGTGCTGGCCACGCTGCACACCAACGACGCGGTGTC
GGCAGTGACGCGGCTGGTGGACATGGGGGTCGAGCCGTTCCTGCTCGCCTCGTCGATGCTTGGCGTACTCGCCCAGCGCC
TGGTGCGGCGCCTGTGCACGCATTGCCGGGTTGAAGAGGACGGCGGCTGGCGCGCGGTCGGCTGCCCGGCTTGCAACCAG
ACCGGCTACAGCGGGCGCACCGGCATCCACGAACTGTTCGTGATCGACGACGAGATCCGCCGCCTGGTCCACCAGGGCCG
CGCCGAGCAGGATCTCCGCGAAGCCGCCCGCGCGGCCGGCATGCGCAGCATGCGCGAGGACGGCGAGCGCTGGATCGCCA
GCGGCTCCACCACCCTGGAGGAAATCCTCCGCGTGACCCGGGACGCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(111-377)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

45.519

90.405

0.412
  pilF Thermus thermophilus HB27

50.928

80.384

0.409
  pilB/pilB1 Synechocystis sp. PCC 6803

41.113

99.574

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.462

83.156

0.403
  pilF Neisseria gonorrhoeae MS11

47.208

84.009

0.397
  pilB Acinetobacter baumannii D1279779

41.403

94.243

0.39
  pilB Vibrio campbellii strain DS40M4

46.329

84.222

0.39
  pilB Vibrio parahaemolyticus RIMD 2210633

46.076

84.222

0.388
  pilB Legionella pneumophila strain ERS1305867

45.501

82.942

0.377
  pilB Acinetobacter baylyi ADP1

45.103

82.729

0.373