Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NRS07_RS05440 Genome accession   NZ_CP103371
Coordinates   1203069..1204832 (-) Length   587 a.a.
NCBI ID   WP_259211659.1    Uniprot ID   -
Organism   Massilia sp. H6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1198069..1209832
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRS07_RS05420 (NRS07_05420) - 1198269..1199366 (-) 1098 WP_259211654.1 GlxA family transcriptional regulator -
  NRS07_RS05425 (NRS07_05425) - 1199478..1200275 (+) 798 WP_259211655.1 MBL fold metallo-hydrolase -
  NRS07_RS05430 (NRS07_05430) - 1200426..1201817 (+) 1392 WP_259211656.1 S8 family serine peptidase -
  NRS07_RS05435 (NRS07_05435) pilC 1201826..1203061 (-) 1236 WP_259211658.1 type II secretion system F family protein Machinery gene
  NRS07_RS05440 (NRS07_05440) pilB 1203069..1204832 (-) 1764 WP_259211659.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NRS07_RS05445 (NRS07_05445) - 1205169..1206731 (-) 1563 WP_259211660.1 methyl-accepting chemotaxis protein -
  NRS07_RS05450 (NRS07_05450) - 1206803..1207210 (-) 408 WP_259211661.1 hypothetical protein -
  NRS07_RS05455 (NRS07_05455) - 1207224..1208480 (-) 1257 WP_259211662.1 porin -
  NRS07_RS05460 (NRS07_05460) uraH 1208722..1209075 (-) 354 WP_259211663.1 hydroxyisourate hydrolase -

Sequence

Protein


Download         Length: 587 a.a.        Molecular weight: 63030.66 Da        Isoelectric Point: 5.2783

>NTDB_id=722371 NRS07_RS05440 WP_259211659.1 1203069..1204832(-) (pilB) [Massilia sp. H6]
MAAVFPNSTTGTQLSAPLSAQMSAQLPALARALVQASRLSAGQADALQKKAASDKTAFIDILLDSGIIDPASLASFCAET
FGYPLLDLSSFAPEAFPLAAIDTKLMQAQRVIALAKRGNKMSVALSDPTNTQALDQIKFQSEAAVEPVIVAHDILLKLLA
GLAKSAEQSLNDLAGEEAAIEFAEEDTQAAAPLDAPSDVEDAPIVRFLNKILMDAVQMGASDIHFEPYEKYYRIRLRVDG
VLRDHAAPPLSIREKLVSRIKVLAKLDISEKRVPQDGRMRLIMGPTRTIDFRVSTLPTLFGEKTVMRILDATQAQMGIDA
LGYDADQKAMLLEAISRPYGMVLVTGPTGSGKTVSLYTCLNVLNKPGINISTAEDPAEINLPGVNQVNVNDRAGLTFPVA
LKSFLRQDPDIIMVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVILITAQRLARRLCT
CKQPLDTSREALVAAGYRDSDLDGGWKPYGPVGCDRCLGSGYKGRVGIYQLMPITPAIESLILTHGNAMEIAAQAEKEGV
NSLRRSGLMKVKQGLTSLEEVLGCTNE

Nucleotide


Download         Length: 1764 bp        

>NTDB_id=722371 NRS07_RS05440 WP_259211659.1 1203069..1204832(-) (pilB) [Massilia sp. H6]
ATGGCAGCAGTCTTTCCCAACTCGACCACTGGCACGCAATTGTCCGCGCCATTGTCGGCGCAGATGTCGGCCCAGCTGCC
GGCACTGGCGCGCGCGCTGGTCCAGGCCAGCCGGTTGAGCGCGGGTCAGGCCGACGCGCTGCAGAAAAAAGCGGCAAGCG
ACAAGACCGCGTTCATCGACATCCTGCTCGACAGCGGCATCATCGACCCGGCCAGCCTGGCGAGCTTCTGCGCCGAGACC
TTCGGCTATCCGCTACTGGACCTGTCGAGCTTCGCACCGGAAGCCTTTCCGCTGGCCGCCATCGACACCAAGCTGATGCA
GGCCCAGCGCGTGATTGCCCTGGCCAAGCGCGGCAACAAGATGTCGGTGGCGCTGTCCGACCCCACCAACACCCAGGCCC
TGGACCAGATTAAATTCCAGAGCGAAGCGGCGGTGGAGCCGGTGATCGTGGCCCACGACATCCTGCTCAAGCTCCTGGCC
GGACTGGCCAAGAGCGCCGAGCAAAGCCTGAACGACCTTGCCGGCGAAGAAGCGGCCATTGAATTTGCCGAGGAAGACAC
CCAGGCTGCCGCCCCGCTGGACGCGCCGAGCGATGTCGAGGACGCGCCGATCGTGCGCTTCCTGAACAAGATTCTGATGG
ATGCGGTGCAGATGGGCGCCTCGGACATCCACTTCGAACCCTACGAAAAATACTATCGCATCCGGCTGCGCGTGGACGGC
GTGCTGCGCGACCATGCTGCGCCGCCGCTGTCGATCCGCGAAAAGCTGGTCTCGCGCATCAAGGTGCTGGCCAAGCTCGA
CATCTCCGAAAAGCGCGTGCCCCAGGACGGCCGCATGCGCCTGATCATGGGCCCGACCCGCACCATCGATTTCCGCGTCT
CGACCCTGCCGACGCTGTTCGGCGAAAAGACCGTGATGCGTATCCTCGACGCCACCCAGGCGCAGATGGGCATCGACGCG
CTCGGCTACGACGCCGACCAGAAGGCGATGCTGCTCGAAGCGATCTCGCGCCCCTACGGCATGGTGCTGGTCACCGGCCC
GACCGGCTCGGGCAAGACCGTGTCGCTGTACACCTGCCTGAACGTGCTCAACAAGCCCGGCATCAACATCTCGACCGCGG
AAGACCCGGCCGAGATCAACCTGCCTGGCGTGAACCAGGTGAACGTCAACGACCGCGCCGGCCTGACCTTCCCGGTGGCC
TTGAAATCGTTTTTGCGCCAGGATCCGGACATCATCATGGTCGGCGAGATCCGCGACCTCGAGACCGCCGACATCGCGAT
CAAGGCTGCCCAGACCGGCCACATGGTGTTTTCGACGCTGCACACCAACGACGCGCCGTCGACCCTGACCCGCCTGATGA
ACATGGGCGTGGCGCCGTTCAACATTGCCTCGTCGGTGATCCTGATTACCGCGCAGCGCCTGGCGCGCCGCCTGTGCACC
TGCAAGCAGCCACTCGACACCAGCCGCGAGGCGCTGGTGGCGGCCGGCTACCGCGACAGCGACCTCGACGGTGGCTGGAA
GCCCTATGGCCCGGTGGGCTGCGACCGCTGCCTCGGCTCGGGCTACAAGGGCCGGGTCGGCATCTACCAGCTGATGCCGA
TCACGCCCGCGATCGAATCCTTGATCCTGACCCACGGCAATGCGATGGAAATCGCCGCCCAGGCCGAGAAGGAAGGCGTC
AATTCGCTGCGCCGCTCGGGCCTGATGAAAGTGAAACAGGGGCTGACCAGCCTCGAAGAAGTGCTCGGCTGTACCAACGA
ATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(62-165)

Type II/IV secretion system protein (T2SSE)

(210-477)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.528

98.978

0.53
  pilB Acinetobacter baylyi ADP1

54.336

96.252

0.523
  pilF Neisseria gonorrhoeae MS11

53.628

96.252

0.516
  pilB Legionella pneumophila strain ERS1305867

47.887

96.763

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

45.536

95.4

0.434
  pilB Vibrio cholerae strain A1552

44.64

96.934

0.433
  pilB Vibrio campbellii strain DS40M4

43.694

95.911

0.419
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.724

98.637

0.392
  pilF Thermus thermophilus HB27

39.888

90.971

0.363