Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   PUN50_RS16060 Genome accession   NZ_CP117988
Coordinates   3482277..3483779 (-) Length   500 a.a.
NCBI ID   WP_038890212.1    Uniprot ID   -
Organism   Vibrio campbellii strain 20220413_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3477277..3488779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN50_RS16030 (PUN50_16030) gspK 3477798..3478808 (-) 1011 WP_043883283.1 type II secretion system minor pseudopilin GspK -
  PUN50_RS16035 (PUN50_16035) gspJ 3478801..3479517 (-) 717 WP_005430083.1 type II secretion system minor pseudopilin GspJ -
  PUN50_RS16040 (PUN50_16040) gspI 3479540..3479935 (-) 396 WP_005430093.1 type II secretion system minor pseudopilin GspI -
  PUN50_RS16045 (PUN50_16045) - 3479916..3480521 (-) 606 WP_005430102.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PUN50_RS16050 (PUN50_16050) gspG 3480553..3480996 (-) 444 WP_005430189.1 type II secretion system major pseudopilin GspG -
  PUN50_RS16055 (PUN50_16055) gspF 3481057..3482277 (-) 1221 WP_005430086.1 type II secretion system inner membrane protein GspF -
  PUN50_RS16060 (PUN50_16060) pilB/pilB1 3482277..3483779 (-) 1503 WP_038890212.1 type II secretion system ATPase GspE Machinery gene
  PUN50_RS16065 (PUN50_16065) gspD 3483772..3485817 (-) 2046 WP_005430082.1 type II secretion system secretin GspD -
  PUN50_RS16070 (PUN50_16070) gspC 3485854..3486777 (-) 924 WP_005430158.1 type II secretion system protein GspC -
  PUN50_RS16075 (PUN50_16075) hslR 3487033..3487419 (+) 387 WP_005430076.1 ribosome-associated heat shock protein Hsp15 -
  PUN50_RS16080 (PUN50_16080) hslO 3487451..3488326 (+) 876 WP_005430180.1 Hsp33 family molecular chaperone HslO -

Sequence

Protein


Download         Length: 500 a.a.        Molecular weight: 55824.06 Da        Isoelectric Point: 5.7709

>NTDB_id=720230 PUN50_RS16060 WP_038890212.1 3482277..3483779(-) (pilB/pilB1) [Vibrio campbellii strain 20220413_1]
MVDMLDTAPSMRRLPFSFANRFKLVLETEHPERPPILYYVEPLNPAALVEVRRVLKQNFIPQVMDKEAFDKKLTEAYQRD
SSEARQLMEDIGADSDDFFSLAEELPHDEDLLESEDDAPIIKLINAMLGEAIKEGASDIHIETFEKTLSIRFRVDGVLRE
VLTPSRKLAPLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTP
SVHDNFRHLIGRPHGIILVTGPTGSGKSTTLYAGLQEINSNERNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAIL
RQDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCSDCKEP
YEADKEQKKLFGLEEHESLVLHRAKGCEVCNQKGYRGRTGIHELLMVDEKVQELIHSEAGEQEIEKAVRAHTPSIRSDGL
SKVSRGITSLEEVMRVTKEA

Nucleotide


Download         Length: 1503 bp        

>NTDB_id=720230 PUN50_RS16060 WP_038890212.1 3482277..3483779(-) (pilB/pilB1) [Vibrio campbellii strain 20220413_1]
ATGGTTGATATGTTAGACACAGCGCCAAGCATGCGCCGTCTGCCATTCAGTTTTGCCAACCGCTTTAAACTGGTTTTGGA
GACGGAGCACCCTGAGCGCCCTCCAATTCTGTATTACGTAGAGCCGCTTAACCCTGCGGCTCTGGTTGAAGTACGTCGAG
TACTGAAACAAAACTTTATTCCTCAAGTGATGGACAAAGAAGCGTTCGATAAAAAGCTCACTGAAGCTTATCAACGTGAT
TCTTCTGAAGCCCGTCAGTTGATGGAAGACATTGGCGCAGACAGTGATGACTTCTTCTCATTAGCAGAAGAACTGCCTCA
CGACGAAGATTTGTTGGAATCGGAAGATGATGCGCCAATCATCAAATTGATTAACGCCATGTTGGGTGAAGCGATCAAAG
AAGGGGCGTCGGATATCCATATTGAGACGTTCGAGAAAACCCTTTCGATTCGTTTCCGTGTCGATGGTGTATTGCGTGAA
GTCTTAACCCCGAGCCGCAAGCTAGCCCCTTTGCTGGTTTCTCGTGTGAAGGTTATGGCGAAGCTGGATATTGCGGAAAA
ACGCGTACCTCAAGATGGTCGTATTTCACTGCGTATCGGTGGTCGTGCGGTGGACGTGCGTGTATCGACCATGCCTTCAT
CGCATGGTGAGCGTGTGGTAATGCGTCTACTGGATAAGAACGCGACTCGTCTTGATCTGCATAGTTTGGGTATGACCCCT
TCAGTGCATGACAACTTCCGTCATTTGATTGGTCGCCCTCACGGCATTATCTTGGTAACCGGTCCGACAGGTTCGGGTAA
GTCGACGACCTTGTACGCGGGCTTGCAAGAGATCAACAGCAATGAACGCAACATTCTGACTGTTGAAGATCCTATCGAAT
TTGATATCGATGGCATCGGCCAAACACAGGTAAACCCTAAGGTTGATATGACCTTTGCGCGTGGTCTACGTGCGATTCTT
CGTCAAGACCCGGATGTAGTGATGGTTGGTGAGATCCGTGACTTGGAGACCGCACAAATTGCCGTGCAAGCCTCTTTGAC
TGGCCACTTAGTGATGTCAACGCTACACACCAACACCGCAGTTGGCGCAATCACGCGTCTGCGCGACATGGGTATTGAAC
CCTTCCTGATCTCTTCTTCGCTATTGGGCGTGCTAGCACAGCGCCTAGTTCGTACCCTTTGTTCTGACTGTAAAGAGCCA
TACGAAGCGGATAAAGAACAGAAGAAACTGTTCGGTCTGGAAGAGCACGAATCGCTGGTTCTGCATCGTGCAAAAGGCTG
TGAAGTGTGTAACCAAAAAGGTTACCGCGGTCGTACTGGTATTCACGAACTTCTAATGGTGGATGAGAAAGTTCAAGAAC
TGATCCACAGTGAAGCGGGTGAACAAGAAATTGAAAAAGCCGTACGAGCGCACACGCCAAGCATTCGTAGTGATGGCCTA
AGCAAAGTCAGTCGTGGTATCACTTCTCTTGAAGAGGTGATGCGAGTGACCAAGGAAGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(126-392)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

43.156

100

0.454
  pilF Thermus thermophilus HB27

44.28

94.4

0.418
  pilB Acinetobacter baumannii D1279779

42.589

95.8

0.408
  pilB Legionella pneumophila strain ERS1305867

47.307

85.4

0.404
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.644

93.8

0.4
  pilB Vibrio parahaemolyticus RIMD 2210633

46.21

81.8

0.378
  pilB Vibrio cholerae strain A1552

47.475

79.2

0.376
  pilB Vibrio campbellii strain DS40M4

45.721

81.8

0.374
  pilB Acinetobacter baylyi ADP1

46.667

78

0.364
  pilF Neisseria gonorrhoeae MS11

46.193

78.8

0.364