Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NWF24_RS04915 Genome accession   NZ_CP102931
Coordinates   1035572..1037305 (-) Length   577 a.a.
NCBI ID   WP_097198199.1    Uniprot ID   A0A286ASI6
Organism   Variovorax paradoxus strain JBCE486     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1030572..1042305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NWF24_RS04895 (NWF24_04895) - 1031171..1032664 (-) 1494 WP_258353222.1 MBOAT family protein -
  NWF24_RS04900 (NWF24_04900) coaE 1032734..1033342 (-) 609 WP_258353223.1 dephospho-CoA kinase -
  NWF24_RS04905 (NWF24_04905) - 1033351..1034316 (-) 966 WP_258353224.1 A24 family peptidase -
  NWF24_RS04910 (NWF24_04910) pilC 1034316..1035545 (-) 1230 WP_258353225.1 type II secretion system F family protein Machinery gene
  NWF24_RS04915 (NWF24_04915) pilB 1035572..1037305 (-) 1734 WP_097198199.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NWF24_RS04925 (NWF24_04925) - 1037547..1038539 (-) 993 WP_258353226.1 polyprenyl synthetase family protein -
  NWF24_RS04930 (NWF24_04930) rplU 1038731..1039042 (+) 312 WP_007833243.1 50S ribosomal protein L21 -
  NWF24_RS04935 (NWF24_04935) rpmA 1039055..1039315 (+) 261 WP_093057448.1 50S ribosomal protein L27 -
  NWF24_RS04940 (NWF24_04940) cgtA 1039387..1040463 (+) 1077 WP_258353227.1 Obg family GTPase CgtA -
  NWF24_RS04945 (NWF24_04945) proB 1040554..1041693 (+) 1140 WP_258353228.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62520.63 Da        Isoelectric Point: 5.5744

>NTDB_id=719823 NWF24_RS04915 WP_097198199.1 1035572..1037305(-) (pilB) [Variovorax paradoxus strain JBCE486]
MAAAELPVKEPSQIALPGLARALVSAGKLPARAAEDIYQKSLSGRTSFIAELTGSGAVSAADLAHTLSTAFGAPLLDLDA
IDPQRLPKDLLDAKLCHAYRIVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMIEAAAVTAAESI
NNIVGSEFEFDDVSADTSGDSNEQAIAEVEDAPVVRFLHKMLLDAVGMRASDIHFEPYEHHYRVRFRVDGELREIASPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVVRILDPSSARLGIDALGYDADEKAR
LLHAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNERAGLTFATALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCTVCRVPADVPR
EALLDAGFREEDLDGTWKPYRPVGCSACSGGYKGRVGIYQVMPITEAIQEIILRDGSALDIAQQSEAEGVRSLRQSGLRK
VMQGLTSLEEVVACTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=719823 NWF24_RS04915 WP_097198199.1 1035572..1037305(-) (pilB) [Variovorax paradoxus strain JBCE486]
ATGGCCGCTGCCGAACTTCCAGTTAAAGAACCCTCGCAGATTGCCCTCCCCGGGCTCGCGCGCGCCCTGGTGTCGGCCGG
CAAGCTTCCTGCCAGGGCCGCCGAAGACATCTACCAGAAGTCCCTGAGTGGCCGCACCAGCTTCATCGCGGAGCTGACCG
GCTCCGGCGCCGTCTCGGCCGCCGATCTCGCCCACACCCTTTCCACCGCCTTCGGCGCGCCGCTGCTCGATCTCGACGCC
ATCGATCCCCAGCGCCTGCCGAAGGATCTGCTCGACGCGAAGCTGTGCCACGCGTACCGCATCGTTGTCCTGAGCAAACG
CAACAACCGTCTGATTGTTGCAACAGCCGATCCCTCGGACCAACAGGCGGCAGAGAAGATCAAGTTCGCCTCCCAGATGG
GTGTCGACTGGGTCATCGCGGAATACGACAAGCTGTCGCGAATGATCGAAGCCGCCGCAGTGACGGCGGCGGAGAGCATC
AACAACATCGTCGGCAGCGAGTTCGAGTTCGACGATGTCTCGGCCGACACCTCGGGCGACAGCAACGAGCAGGCAATCGC
AGAAGTCGAAGACGCACCGGTCGTGCGCTTCCTTCACAAGATGCTGCTCGACGCCGTTGGCATGCGGGCCTCGGACATTC
ACTTCGAGCCCTACGAGCATCACTACCGCGTGCGTTTCCGGGTCGACGGCGAACTGCGCGAGATCGCCAGCCCGCCCACC
GTCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTCATTTCCCGGCTCGACATCTCCGAGAAACGCGTGCCGCAGGACGG
CCGAATGAAGCTCAAGATCGGGCCCGACCGCGTGATCGACTTTCGCGTGAGCACACTGCCCACGCTGTTTGGCGAGAAGA
TCGTCGTTCGTATTCTCGACCCGAGCAGCGCGCGCCTGGGTATCGATGCCCTGGGCTACGACGCGGATGAAAAAGCGCGG
CTCCTGCATGCCATCGGCCGCCCGTACGGGATGGTGCTCGTCACCGGCCCGACTGGCTCCGGCAAGACAGTGTCGCTCTA
CACCTGCCTGAACCTGCTGAACCAGCCGGGCGTCAACATCGCCACGGCGGAAGATCCGTCGGAAATCAACCTGCCCGGCG
TCAACCAGGTCAACGTCAACGAACGGGCCGGCCTGACCTTCGCGACCGCGCTGCGCGCCTTCCTGCGCCAGGATCCCGAC
ATCATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGATATCTCGATCAAGGCCGCGCAAACGGGCCACCTGGTGCT
CTCCACGCTGCACACCAACGACGCGCCGACCACGCTCACGCGGATGCGCAACATGGGTATCGCGCCGTTCAACATCGCAT
CCAGCGTGATCCTGATCACGGCACAGCGCCTCGCGCGGCGGCTGTGTACCGTCTGCCGCGTGCCGGCCGACGTCCCGCGC
GAAGCGCTACTCGACGCAGGGTTCAGGGAGGAAGATCTGGACGGCACATGGAAGCCCTACCGCCCCGTCGGCTGCTCCGC
TTGCAGCGGCGGCTACAAGGGCCGCGTGGGCATCTACCAGGTGATGCCGATCACCGAGGCCATCCAGGAAATCATCCTGC
GGGACGGCAGCGCGCTCGACATCGCACAGCAGTCCGAGGCGGAGGGCGTACGCTCGCTGCGGCAGTCGGGTCTGCGCAAA
GTGATGCAAGGTCTCACTTCTCTGGAAGAAGTCGTGGCCTGCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-152)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A286ASI6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.545

97.227

0.53
  pilB Acinetobacter baumannii D1279779

54.545

97.227

0.53
  pilF Neisseria gonorrhoeae MS11

52.575

97.574

0.513
  pilB Legionella pneumophila strain ERS1305867

49.554

97.227

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

45.745

97.747

0.447
  pilB Vibrio cholerae strain A1552

47.77

93.241

0.445
  pilB Vibrio campbellii strain DS40M4

45.471

97.574

0.444
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.7

94.107

0.383
  pilF Thermus thermophilus HB27

37.413

99.133

0.371