Detailed information NTG0071944

Bioinformatically predicted

Overview

• Name: dprA/cilB/dalA
• Type: Machinery gene
• Locus tag: EL263_RS06170
• Genome accession: NZ_AP018938
• Coordinates: 1173235..1174083 (-)
• Length: 282 a.a.
• NCBI ID: WP_000705304.1
• Uniprot ID: A0A0E7P1X9
• Organism: Streptococcus pneumoniae strain ATCC 49619
• Function: ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology) DNA processing Competence regulation

Genomic Context

Location: 1168235..1179083

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL263_RS06155 (SPAT_1165)	-	1168589..1168948 (-)	360	WP_001237219.1	YbaN family protein	-
EL263_RS06160 (SPAT_1166)	topA	1169054..1171141 (-)	2088	WP_000179644.1	type I DNA topoisomerase	-
EL263_RS06165 (SPAT_1167)	-	1171308..1172351 (-)	1044	WP_001842104.1	TIGR00341 family protein	-
EL263_RS06170 (SPAT_1168)	dprA/cilB/dalA	1173235..1174083 (-)	849	WP_000705304.1	DNA-processing protein DprA	Machinery gene
EL263_RS06175 (SPAT_1169)	-	1174179..1174868 (-)	690	WP_000643970.1	sugar phosphate nucleotidyltransferase	-
EL263_RS06180 (SPAT_1170)	-	1174880..1175758 (-)	879	WP_000837618.1	DMT family transporter	-
EL263_RS06185 (SPAT_1171)	licA	1175748..1176617 (-)	870	WP_000411215.1	choline kinase LicA	-
EL263_RS06190 (SPAT_1172)	-	1176634..1177656 (-)	1023	WP_000609885.1	ribitol-5-phosphate dehydrogenase	-
EL263_RS06195 (SPAT_1173)	-	1177661..1178368 (-)	708	WP_000638502.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 31076.75 Da
• Isoelectric Point: 5.4047

>NTDB_id=71944 EL263_RS06170 WP_000705304.1 1173235..1174083(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain ATCC 49619]
MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPGSILDGLSDGCHHLIQEGAKLLTSGQDVLAEFEF

Nucleotide

• Length: 849 bp

>NTDB_id=71944 EL263_RS06170 WP_000705304.1 1173235..1174083(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain ATCC 49619]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAGGTTTGACCAATCAACAGATTTTGAAAGTGCTAGAATA
CGGTGAAAATGTTGATCAGGAGCTTTTGTTGGGTGATATTGCAGATATATCAGGTTGCCGTAATCCAGCCGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGTCGAAAGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TATCCTTGGGATTTGAGTGAAATATATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTTGACCTCCTGAAATTCCC
GAAGGTAGCGGTCGTGGGCAGTCGTGCTTGTAGCAAACAGGGAGCTAAGTCAGTTGAAAAAGTCATTCAAGGCTTGGAAA
ATGAACTGGTTATTGTCAGTGGTCTGGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTTCAGAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTACATCGGCAATGA
CCATCTGGTTCTAAGTGAATATGGACCTGGCGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGTTCAGGTAGTCTCATTACGTGTGAGCGAGCAATGGAAGAA
GGACGCGATGTCTTTGCTATTCCTGGTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGAGC
AAAATTGCTCACCAGTGGGCAAGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (75-271)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (3-64)

3D structure

• AlphaFold DB: A0A0E7P1X9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	99.645	100	0.996
dprA/cilB/dalA	Streptococcus pneumoniae D39	99.645	100	0.996
dprA/cilB/dalA	Streptococcus pneumoniae R6	99.645	100	0.996
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	99.645	100	0.996
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	98.227	100	0.982
dprA/cilB/dalA	Streptococcus mitis SK321	97.518	100	0.975
dprA	Streptococcus mutans UA159	63.214	99.291	0.628
dprA	Lactococcus lactis subsp. cremoris KW2	55.357	99.291	0.55
dprA	Staphylococcus aureus N315	37.456	100	0.376