Detailed information NTG0718076

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: NUW85_RS18665
• Genome accession: NZ_CP102603
• Coordinates: 3785675..3786289 (+)
• Length: 204 a.a.
• NCBI ID: WP_095318411.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain MBLB0692
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3780675..3791289

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NUW85_RS18630 (NUW85_18635)	aroE	3781362..3782204 (+)	843	WP_025852995.1	shikimate dehydrogenase	-
NUW85_RS18635 (NUW85_18640)	yhbY	3782198..3782488 (+)	291	WP_139885324.1	ribosome assembly RNA-binding protein YhbY	-
NUW85_RS18640 (NUW85_18645)	-	3782499..3783068 (+)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
NUW85_RS18645 (NUW85_18650)	yqeK	3783058..3783639 (+)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
NUW85_RS18650 (NUW85_18655)	rsfS	3783636..3783992 (+)	357	WP_003152864.1	ribosome silencing factor	-
NUW85_RS18655 (NUW85_18660)	-	3783989..3784726 (+)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
NUW85_RS18660 (NUW85_18665)	comER	3784795..3785616 (-)	822	WP_003152866.1	late competence protein ComER	-
NUW85_RS18665 (NUW85_18670)	comEA	3785675..3786289 (+)	615	WP_095318411.1	helix-hairpin-helix domain-containing protein	Machinery gene
NUW85_RS18670 (NUW85_18675)	-	3786356..3786925 (+)	570	WP_003152868.1	ComE operon protein 2	-
NUW85_RS18675 (NUW85_18680)	comEC	3786926..3789277 (+)	2352	WP_207119962.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
NUW85_RS18680 (NUW85_18685)	-	3789296..3789430 (-)	135	WP_003152870.1	YqzM family protein	-
NUW85_RS18685 (NUW85_18690)	-	3789471..3789625 (+)	155	Protein_3622	hypothetical protein	-
NUW85_RS18690 (NUW85_18695)	holA	3789665..3790706 (+)	1042	Protein_3623	DNA polymerase III subunit delta	-
NUW85_RS18695 (NUW85_18700)	rpsT	3790723..3790989 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22250.43 Da
• Isoelectric Point: 6.0523

>NTDB_id=718076 NUW85_RS18665 WP_095318411.1 3785675..3786289(+) (comEA) [Bacillus amyloliquefaciens strain MBLB0692]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=718076 NUW85_RS18665 WP_095318411.1 3785675..3786289(+) (comEA) [Bacillus amyloliquefaciens strain MBLB0692]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363