Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NRZ33_RS02370 Genome accession   NZ_CP102364
Coordinates   513568..515274 (-) Length   568 a.a.
NCBI ID   WP_268032076.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain BC13     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 508568..520274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRZ33_RS02340 (NRZ33_02340) mutT 509245..509658 (+) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -
  NRZ33_RS02345 (NRZ33_02345) yacG 509719..509913 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  NRZ33_RS02350 (NRZ33_02350) zapD 509923..510645 (-) 723 WP_268032072.1 cell division protein ZapD -
  NRZ33_RS02355 (NRZ33_02355) coaE 510683..511297 (-) 615 WP_268032073.1 dephospho-CoA kinase -
  NRZ33_RS02360 (NRZ33_02360) pilD 511316..512188 (-) 873 WP_268032074.1 prepilin peptidase Machinery gene
  NRZ33_RS02365 (NRZ33_02365) pilC 512211..513452 (-) 1242 WP_268032075.1 type II secretion system F family protein Machinery gene
  NRZ33_RS02370 (NRZ33_02370) pilB 513568..515274 (-) 1707 WP_268032076.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  NRZ33_RS02375 (NRZ33_02375) - 515278..515742 (-) 465 WP_045788906.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NRZ33_RS02380 (NRZ33_02380) nadC 516064..516921 (-) 858 WP_268032077.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NRZ33_RS02385 (NRZ33_02385) - 516927..517397 (-) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  NRZ33_RS02390 (NRZ33_02390) ampD 517544..518116 (+) 573 WP_268032078.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NRZ33_RS02395 (NRZ33_02395) pdhR 518392..519156 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62501.86 Da        Isoelectric Point: 5.4342

>NTDB_id=716438 NRZ33_RS02370 WP_268032076.1 513568..515274(-) (pilB) [Aeromonas hydrophila strain BC13]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELFELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=716438 NRZ33_RS02370 WP_268032076.1 513568..515274(-) (pilB) [Aeromonas hydrophila strain BC13]
ATGACATCTAGCCCCAATAGCGGCCTGGCGTTAAGCTTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
TCTGAGCCAAGCCAAGGCGCAGCGTAAACCCTTTGTGACCTTCCTGATCGAAAATGAAATCCTCGACAGCAAGGCATTGG
CTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAAAAACATCACGTCCTGCCCATCTATACCCAGGGTCACACCCTCTATATCGCCATGTCGGA
TCCCACCAATGTGTCTGCACTGGAGGATTTCGGCTTCAGCTTCGGGTTGCATACCGAGGCACTGCTGGTAGAGGAAAATA
AGCTCACCGCCGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAGCTGGAAGTGTCTGACGAGGGATCGCGGCTCGACGAGAGCGTCAATACGGCCGACGATGACGCGCCCATCGTCAA
ATACATCAACAAAATAATGATGGACGCCATCAAGCGCGGTGCCTCTGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAGGTAGCAACCCCACCGGTCAATCTGGCCAACCGCTTCTCGGCACGC
CTCAAGGTGATGGCACGGCTCGATATCGCCGAACGGCGACTGCCCCAGGATGGTCGCATCAAGCTCAAGCTGTCGCGCAA
CAAATCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCGACTCCTCCG
CTGCGCGCCTCAATATCGAGCAGCTCGGTTTTGACGATCGGCAGAAGGCGCAATATCTGCGTGCTCTCTCGAAGCCTCAG
GGCATGATTCTGGTGACCGGCCCGACCGGTTCTGGCAAGACGGTGTCGCTCTACACCGGCCTGAACATTCTCAACACCAC
AGAAGTAAATATTTCGACCGCCGAAGACCCGGTGGAGATCAACCTGCCAGGGGTAAATCAGGTACAGGTCAATCCCAAGG
CGGGGCTGACCTTCGCCAGTGCCCTGCGCTCCTTTCTGCGCCAGGATCCCGATGTGGTCATGGTGGGTGAAATCCGGGAT
CTGGAAACCGCCGAAATTGCCATCAAGGCCGCCCAGACAGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
GGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACTCTGATCATGGCTCAGC
GGCTGGCGCGTAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTGCCAGAGGCTGAGCTGTTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCGGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCGGGTGGGTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAGATTGCCG
CCATCGCCCAGAAGGAGGGCATGCGCACCTTGCGCACTTCTGGCCTTGAGAAGGCCCGCCTGGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.36

100

0.555
  pilB Acinetobacter baylyi ADP1

55.088

100

0.553
  pilB Legionella pneumophila strain ERS1305867

54.69

99.472

0.544
  pilB Vibrio cholerae strain A1552

54.336

99.472

0.54
  pilB Vibrio campbellii strain DS40M4

51.943

99.648

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.246

98.944

0.507
  pilF Neisseria gonorrhoeae MS11

46.631

99.296

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.341

92.958

0.375