Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NQ186_RS25410 Genome accession   NZ_CP102358
Coordinates   5725395..5727095 (+) Length   566 a.a.
NCBI ID   WP_257356139.1    Uniprot ID   -
Organism   Pseudomonas zeae strain BIM B-582     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5720395..5732095
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQ186_RS25390 (NQ186_25390) - 5720550..5723501 (+) 2952 WP_257356138.1 DUF748 domain-containing protein -
  NQ186_RS25395 (NQ186_25395) - 5723501..5723797 (+) 297 WP_093434887.1 DUF2845 domain-containing protein -
  NQ186_RS25400 (NQ186_25400) - 5724083..5724439 (-) 357 WP_077574577.1 BON domain-containing protein -
  NQ186_RS25405 (NQ186_25405) - 5724737..5725168 (-) 432 WP_038369043.1 pilin -
  NQ186_RS25410 (NQ186_25410) pilB 5725395..5727095 (+) 1701 WP_257356139.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NQ186_RS25415 (NQ186_25415) pilC 5727098..5728315 (+) 1218 WP_093434875.1 type II secretion system F family protein Machinery gene
  NQ186_RS25420 (NQ186_25420) pilD 5728317..5729189 (+) 873 WP_093434872.1 A24 family peptidase Machinery gene
  NQ186_RS25425 (NQ186_25425) coaE 5729186..5729809 (+) 624 WP_257356140.1 dephospho-CoA kinase -
  NQ186_RS25430 (NQ186_25430) yacG 5729806..5730006 (+) 201 WP_095048684.1 DNA gyrase inhibitor YacG -
  NQ186_RS25435 (NQ186_25435) - 5730013..5730228 (-) 216 WP_007959584.1 hypothetical protein -
  NQ186_RS25440 (NQ186_25440) - 5730294..5730983 (-) 690 WP_099757210.1 energy-coupling factor ABC transporter permease -
  NQ186_RS25445 (NQ186_25445) - 5730980..5731447 (-) 468 WP_257356141.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62721.72 Da        Isoelectric Point: 5.9184

>NTDB_id=716406 NQ186_RS25410 WP_257356139.1 5725395..5727095(+) (pilB) [Pseudomonas zeae strain BIM B-582]
MNDIALSGLAKQLVQAELLTEKGAQQAWQQAQRNRLSLVSYLVQNKLVKSWQVAEIASEHFGMALLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHTSGLEDMADVDLDG
VDVESVDDSKQDTIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKAIEIPRETLLKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDLQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=716406 NQ186_RS25410 WP_257356139.1 5725395..5727095(+) (pilB) [Pseudomonas zeae strain BIM B-582]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTCCAGGCCGAACTGCTCACGGAAAAAGGCGCCCAGCAAGC
CTGGCAACAGGCCCAGCGCAATCGCCTGTCGCTGGTCAGCTATCTGGTGCAGAACAAACTGGTCAAGAGCTGGCAGGTGG
CCGAGATTGCCAGTGAGCATTTCGGCATGGCCTTGCTTGATCTCAATTGCCTGGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGCGAAAAACTCGTGCGTCAGCATCACGCCCTGCCCTTGTGGCGGCGTGGCAACAAGCTGTTCGTGGGCATTTCCGA
CCCGAGCAATCATCAAGCAATCAACGACATTCAATTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTCGAGGACGACA
AACTTACCGATGCCATCGAAAAATTCTTCGACACCCACACCAGCGGTCTCGAGGACATGGCCGATGTCGATCTCGACGGG
GTCGATGTCGAATCCGTCGACGACAGCAAGCAGGACACCATCGGCGGCCTCGACGCCGACGATGCGCCGGTGGTGCGCTT
CGTGCACAAGATGCTGCTCGACGCGATCAAAAGCGGTTCTTCCGACTTGCACTTCGAGCCTTACGAAAAAAACTACCGGG
TGCGGGTGCGCACCGACGGCATCCTGCGCGAGGTTGCCAAACCGCCGATTCAACTGGCCGGGCGCATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTGGATATTTCGGAACGGCGCAAACCTCAGGACGGGCGGATCAAGATGCGCCTGTCGAAAAGCAA
GTCGATCGATTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGCG
CGCAGATCGGTATCGACGCCCTCGGCTATGAGCCCGAGCAAAAGGATCTGTACATGGCCGCACTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGGCCCACCGGTTCGGGGAAAACCGTTTCGCTGTACACCGGCCTGAATATTCTCAACACCGTCGA
CATCAATATCTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAGCAAG
GGCTCGACTTTGCCCAGGCATTGCGCTCGTTTCTGCGTCAGGACCCAGACGTGATCATGGTCGGTGAAATCCGCGACCTG
GAAACCGCCGAGATCGCCATCAAAGCCGCGCAGACCGGTCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCTGCGGA
AACCCTGACGCGCCTGCACAACATGGGCATTCCCGGTTTCAACATCGCCACCTCGGTCAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAACTGTGCAGCCACTGCAAGAAAGCCATCGAGATCCCGCGCGAAACCTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAACGGCGGTTACAAAGGACGCGTGGGGAT
TTATGAAGTGGTGAAAAACACACCGGAGCTGCAACGCTTGATCATGGCTGAAGGCAACTCGCTGGAAATCGACCTGCAGA
TGCGCCGCGACGGCTTCGACGACCTGCGCACCTCCGGGCTGCACAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.97

99.47

0.587
  pilB Acinetobacter baumannii D1279779

57.979

99.647

0.578
  pilB Legionella pneumophila strain ERS1305867

56.007

100

0.56
  pilB Vibrio cholerae strain A1552

51.327

99.823

0.512
  pilF Neisseria gonorrhoeae MS11

49.911

99.47

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.47

0.496
  pilB Vibrio campbellii strain DS40M4

48.862

100

0.493
  pilF Thermus thermophilus HB27

36.918

98.587

0.364