Detailed information NTG0716353

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: NRF13_RS12845
• Genome accession: NZ_CP102350
• Coordinates: 2592530..2593144 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain PMC206
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2587530..2598144

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NRF13_RS12815 (NRF13_12815)	rpsT	2587830..2588096 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
NRF13_RS12820 (NRF13_12820)	holA	2588113..2589154 (-)	1042	Protein_2462	DNA polymerase III subunit delta	-
NRF13_RS12825 (NRF13_12825)	-	2589194..2589348 (-)	155	Protein_2463	hypothetical protein	-
NRF13_RS12830 (NRF13_12830)	-	2589389..2589523 (+)	135	WP_003152870.1	YqzM family protein	-
NRF13_RS12835 (NRF13_12835)	comEC	2589542..2591893 (-)	2352	WP_058906194.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
NRF13_RS12840 (NRF13_12840)	-	2591894..2592463 (-)	570	WP_003152868.1	ComE operon protein 2	-
NRF13_RS12845 (NRF13_12845)	comEA	2592530..2593144 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
NRF13_RS12850 (NRF13_12850)	comER	2593203..2594024 (+)	822	WP_014305449.1	late competence protein ComER	-
NRF13_RS12855 (NRF13_12855)	-	2594093..2594830 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
NRF13_RS12860 (NRF13_12860)	rsfS	2594827..2595183 (-)	357	WP_003152864.1	ribosome silencing factor	-
NRF13_RS12865 (NRF13_12865)	yqeK	2595180..2595761 (-)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
NRF13_RS12870 (NRF13_12870)	-	2595751..2596320 (-)	570	WP_058906195.1	nicotinate-nucleotide adenylyltransferase	-
NRF13_RS12875 (NRF13_12875)	yhbY	2596331..2596621 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
NRF13_RS12880 (NRF13_12880)	aroE	2596615..2597457 (-)	843	WP_025852995.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=716353 NRF13_RS12845 WP_003152867.1 2592530..2593144(-) (comEA) [Bacillus velezensis strain PMC206]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=716353 NRF13_RS12845 WP_003152867.1 2592530..2593144(-) (comEA) [Bacillus velezensis strain PMC206]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363