Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NRL03_RS18525 Genome accession   NZ_CP102315
Coordinates   3971558..3972667 (+) Length   369 a.a.
NCBI ID   WP_319858192.1    Uniprot ID   -
Organism   Aeromonas caviae strain BC01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3966558..3977667
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRL03_RS18490 (NRL03_18520) - 3966593..3967423 (+) 831 WP_201921265.1 CPBP family intramembrane glutamic endopeptidase -
  NRL03_RS18495 (NRL03_18525) - 3967499..3967918 (-) 420 WP_029313762.1 DUF4426 domain-containing protein -
  NRL03_RS18500 (NRL03_18530) yggU 3967937..3968236 (-) 300 WP_104455228.1 DUF167 family protein YggU -
  NRL03_RS18505 (NRL03_18535) - 3968236..3968787 (-) 552 WP_010673053.1 YggT family protein -
  NRL03_RS18510 (NRL03_18540) proC 3968811..3969635 (-) 825 WP_279976539.1 pyrroline-5-carboxylate reductase -
  NRL03_RS18515 (NRL03_18545) - 3969759..3970460 (-) 702 WP_042864460.1 YggS family pyridoxal phosphate-dependent enzyme -
  NRL03_RS18520 (NRL03_18550) pilT 3970500..3971534 (+) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  NRL03_RS18525 (NRL03_18555) pilU 3971558..3972667 (+) 1110 WP_319858192.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NRL03_RS18530 (NRL03_18560) yaaA 3972700..3973473 (+) 774 WP_279976540.1 peroxide stress protein YaaA -
  NRL03_RS18535 (NRL03_18565) srmB 3973661..3974881 (-) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  NRL03_RS18540 (NRL03_18570) - 3975009..3975719 (+) 711 WP_201871603.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  NRL03_RS18545 (NRL03_18575) brnQ 3975955..3977217 (+) 1263 WP_045523295.1 branched-chain amino acid transport system II carrier protein -
  NRL03_RS18550 (NRL03_18580) - 3977308..3977535 (-) 228 WP_039039956.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40842.02 Da        Isoelectric Point: 6.4405

>NTDB_id=716061 NRL03_RS18525 WP_319858192.1 3971558..3972667(+) (pilU) [Aeromonas caviae strain BC01]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHGRCLVTQREVGIDTGSFDVALRNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGLRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=716061 NRL03_RS18525 WP_319858192.1 3971558..3972667(+) (pilU) [Aeromonas caviae strain BC01]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGGCCGGCTCCCCTCGACAAGACGACGGCGCTGGCGTTGATCAGGGAGA
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGGGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCACCAGCACGGCCGCTGCCTGGTGACCCAACGGGAGGTCGGCATCGACACCGGCTCCTTCGACGT
GGCGCTCAGAAACTCCCTGCGCCAGGCACCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGACACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCACCTGGTTCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCATGGGCTGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

58.689

95.122

0.558
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

91.057

0.39
  pilT Legionella pneumophila strain Lp02

41.108

92.954

0.382
  pilT Legionella pneumophila strain ERS1305867

41.108

92.954

0.382