Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LOY40_RS24600 Genome accession   NZ_CP102179
Coordinates   5471485..5473185 (+) Length   566 a.a.
NCBI ID   WP_258648293.1    Uniprot ID   -
Organism   Pseudomonas alvandae strain B21-020     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5466485..5478185
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY40_RS24580 (LOY40_24580) - 5466588..5469521 (+) 2934 WP_258648292.1 DUF748 domain-containing protein -
  LOY40_RS24585 (LOY40_24585) - 5469523..5469822 (+) 300 WP_030140249.1 DUF2845 domain-containing protein -
  LOY40_RS24590 (LOY40_24590) - 5470138..5470491 (-) 354 WP_030140250.1 BON domain-containing protein -
  LOY40_RS24595 (LOY40_24595) - 5470836..5471258 (-) 423 WP_030140251.1 pilin -
  LOY40_RS24600 (LOY40_24600) pilB 5471485..5473185 (+) 1701 WP_258648293.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOY40_RS24605 (LOY40_24605) pilC 5473188..5474405 (+) 1218 WP_030140253.1 type II secretion system F family protein Machinery gene
  LOY40_RS24610 (LOY40_24610) pilD 5474407..5475276 (+) 870 WP_258648294.1 A24 family peptidase Machinery gene
  LOY40_RS24615 (LOY40_24615) coaE 5475276..5475899 (+) 624 WP_030140255.1 dephospho-CoA kinase -
  LOY40_RS24620 (LOY40_24620) yacG 5475896..5476102 (+) 207 WP_030140256.1 DNA gyrase inhibitor YacG -
  LOY40_RS24625 (LOY40_24625) - 5476174..5476389 (-) 216 WP_030140257.1 hypothetical protein -
  LOY40_RS24630 (LOY40_24630) - 5476453..5477139 (-) 687 WP_030140258.1 energy-coupling factor ABC transporter permease -
  LOY40_RS24635 (LOY40_24635) - 5477211..5477624 (-) 414 WP_030140259.1 tautomerase family protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62672.67 Da        Isoelectric Point: 5.7006

>NTDB_id=715304 LOY40_RS24600 WP_258648293.1 5471485..5473185(+) (pilB) [Pseudomonas alvandae strain B21-020]
MNDIALSGLAKQLVLAELITEQSAQQAFQQAQRNRVPLVSYLVQNKLIQSRQVAEIASEHFGIALLDLNSLDKDNQPAGL
VSEKLVRQHHALPLWRRGNKLYVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSSTGLEGMADVDLDG
LDIESTDDRKQDSIVGQDGDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKTYRVRVRTDGMLREVAKPPIQLANRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAHALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLVRLHNMGIPGFNIATAVHLIIAQRLARKLCTHCKKAIEIPEETLLKEGFPR
ERIGSFTIYEPVGCEQCNNGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLAKVMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=715304 LOY40_RS24600 WP_258648293.1 5471485..5473185(+) (pilB) [Pseudomonas alvandae strain B21-020]
ATGAATGACATCGCCCTCAGCGGCCTGGCCAAGCAATTGGTGCTGGCCGAACTGATCACCGAACAAAGCGCGCAGCAGGC
TTTTCAACAGGCCCAGCGCAATCGAGTGCCCCTGGTCAGTTACCTGGTGCAGAACAAACTGATCCAGAGCCGGCAGGTGG
CGGAAATCGCCTCGGAGCATTTCGGTATAGCCCTGCTGGACCTCAACAGCCTCGACAAGGACAACCAGCCCGCAGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCATCACGCCCTGCCGCTGTGGCGGCGCGGCAACAAGCTGTACGTGGGCATTTCCGA
CCCCACTAACCATCAAGCCATCAACGACATCCAGTTCAGCACCGGCCTGACCACCGAAGCCATCCTGGTGGAAGACGACA
AGCTCAGCGATGCCATCGAGAAATTCTTCGAGTCCAGCAGCACCGGCCTGGAGGGCATGGCCGACGTCGACCTCGACGGC
CTGGACATCGAGTCGACCGATGATCGCAAGCAGGACAGCATCGTCGGACAGGACGGCGACGATGCGCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTGGATGCCATCAAGGGTGGCTCTTCCGACCTGCACTTCGAACCCTATGAAAAAACCTACCGGG
TCCGGGTGCGCACCGACGGCATGCTGCGGGAGGTCGCCAAGCCCCCCATCCAACTGGCCAACCGTATTGCCGCGCGCCTC
AAGGTCATGGCCAGCCTGGACATTTCCGAGCGGCGCAAACCCCAGGACGGTCGCTTGAAGATGCGCCTGTCAAAAACCAA
GTCCATTGATTTCCGGGTCAACACCTTGCCCACGCTCTGGGGCGAAAAAGTGGTGATCCGGATCCTCGACCCCTCCAGTG
CGCAAATGGGCATCGACGCCCTGGGCTATGAGCCGGAACAGAAAGACTTGTACATGGCCGCCCTCAAGCAGCCGCAGGGA
CTGATCCTGGTGACCGGGCCGACCGGTTCGGGCAAGACCGTGTCGCTCTACACCGGTTTGAACATCCTCAACACCGTGGA
CATCAATATCTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAAGTCAACGTGAACCCACGCCAGG
GCCTGGATTTTGCCCATGCCTTGCGCTCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAAATCGCCATCAAGGCCGCCCAGACCGGGCACCTGGTGCTGTCCACCCTGCACACCAACAGCGCCGCCGA
AACCCTGGTCCGCCTGCATAACATGGGCATCCCAGGGTTCAACATCGCCACGGCGGTGCACCTGATCATCGCCCAGCGCC
TGGCGCGAAAATTGTGTACGCATTGCAAGAAAGCCATCGAGATTCCCGAGGAAACCCTGCTCAAGGAAGGTTTCCCCCGG
GAACGCATCGGCTCATTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAACAACGGCTACAAAGGTCGCGTGGGGGT
ATACGAAGTGGTCAAGAACACGCCGGAGCTGCAACGGTTGATCATGGCCGAGGGCAACTCCCTGGAAATCGACCTGCAAA
TGCGCAAGGACGGCTTCAACGACCTGCGTACGTCGGGGCTGGCCAAAGTGATGCAAGGCATCACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.938

99.823

0.588
  pilB Acinetobacter baumannii D1279779

57.496

100

0.576
  pilB Legionella pneumophila strain ERS1305867

55.106

100

0.553
  pilB Vibrio cholerae strain A1552

50.707

100

0.507
  pilB Vibrio campbellii strain DS40M4

47.719

100

0.481
  pilF Neisseria gonorrhoeae MS11

48.135

99.47

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

48.561

98.233

0.477
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.852

98.763

0.364
  pilF Thermus thermophilus HB27

36.042

100

0.36