Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LOY64_RS25300 Genome accession   NZ_CP102177
Coordinates   5693423..5695123 (+) Length   566 a.a.
NCBI ID   WP_265068283.1    Uniprot ID   -
Organism   Pseudomonas corrugata strain B21-055     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5688423..5700123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY64_RS25280 (LOY64_25295) - 5688591..5691524 (+) 2934 WP_265068282.1 DUF748 domain-containing protein -
  LOY64_RS25285 (LOY64_25300) - 5691526..5691822 (+) 297 WP_053192127.1 DUF2845 domain-containing protein -
  LOY64_RS25290 (LOY64_25305) - 5692126..5692479 (-) 354 WP_024779584.1 BON domain-containing protein -
  LOY64_RS25295 (LOY64_25310) - 5692777..5693199 (-) 423 WP_024779583.1 pilin -
  LOY64_RS25300 (LOY64_25315) pilB 5693423..5695123 (+) 1701 WP_265068283.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOY64_RS25305 (LOY64_25320) pilC 5695126..5696343 (+) 1218 WP_265068284.1 type II secretion system F family protein Machinery gene
  LOY64_RS25310 (LOY64_25325) pilD 5696345..5697214 (+) 870 WP_265068285.1 A24 family peptidase Machinery gene
  LOY64_RS25315 (LOY64_25330) coaE 5697225..5697848 (+) 624 WP_024779579.1 dephospho-CoA kinase -
  LOY64_RS25320 (LOY64_25335) yacG 5697845..5698051 (+) 207 WP_024779578.1 DNA gyrase inhibitor YacG -
  LOY64_RS25325 (LOY64_25340) - 5698056..5698271 (-) 216 WP_024779577.1 hypothetical protein -
  LOY64_RS25330 (LOY64_25345) - 5698335..5699021 (-) 687 WP_024779576.1 energy-coupling factor ABC transporter permease -
  LOY64_RS25335 (LOY64_25350) - 5699356..5699982 (+) 627 WP_024779575.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62664.52 Da        Isoelectric Point: 5.1456

>NTDB_id=715274 LOY64_RS25300 WP_265068283.1 5693423..5695123(+) (pilB) [Pseudomonas corrugata strain B21-055]
MNDIALSGLTKQLVLAELITEQSAQQAQQQAQRNRMPLVSYLVQNRLVQSRQVAEIASEHFGVALLDLNSLDKESQPTGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSTTGLEGMGDVDLDG
LDIESDDDRKQDAIAGQDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRVRTDGMLREVARPPTQLAGRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKELYLAALKQPQG
LILVTGPTGSGKTVSLYTDLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCNQCKKATEIPEETLLKEGFPK
ERIGSFTIYEPVGCEQCNSGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=715274 LOY64_RS25300 WP_265068283.1 5693423..5695123(+) (pilB) [Pseudomonas corrugata strain B21-055]
ATGAATGACATCGCCCTCAGCGGTTTGACCAAGCAACTGGTACTGGCCGAGCTGATCACCGAGCAAAGCGCGCAGCAGGC
GCAGCAGCAAGCCCAACGCAACCGCATGCCCCTGGTCAGCTACCTTGTGCAGAACAGATTGGTCCAGAGTCGCCAGGTTG
CTGAAATTGCCTCGGAGCATTTCGGCGTCGCCCTGCTGGACCTCAACAGCCTGGACAAGGAAAGCCAGCCCACCGGCCTT
GTCAGTGAAAAACTGGTTCGGCAACACCATGCCCTGCCACTGTGGCGGCGCGGCAACAAGCTGTTCGTGGGTATCTCCGA
CCCCACCAACCACCAGGCCATCAACGACATCCAGTTCAGTACCGGCCTGACCACCGAAGCCATCCTGGTAGAGGACGACA
AACTCAGCGATGCCATCGAGAAGTTTTTCGAATCCAGCACCACCGGCCTGGAAGGCATGGGGGATGTCGACCTCGACGGC
CTGGACATCGAGTCGGACGACGATCGCAAACAGGACGCCATTGCCGGCCAGGACGCTGACGATGCGCCGGTGGTGCGGTT
CGTCAACAAGATGCTGCTGGACGCGATCAAGGGTGGCTCTTCCGACCTGCACTTCGAACCCTATGAGAAATCCTACCGGG
TGCGGGTACGTACCGACGGCATGTTGCGGGAGGTGGCGAGGCCGCCGACCCAACTGGCCGGCCGCATCGCCGCGCGCCTG
AAGGTCATGGCCAGCCTGGATATTTCCGAGCGGCGCAAGCCGCAGGACGGCCGGTTGAAAATGCGCCTGTCGAAAACCAA
GTCCATCGACTTCCGGGTCAATACCCTGCCCACGCTCTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCTTCCAGCG
CGCAAATGGGTATCGATGCCCTGGGCTACGAACCGGATCAGAAAGAGCTCTACCTCGCGGCCCTCAAACAACCCCAAGGG
CTGATCCTGGTCACCGGGCCGACCGGCTCGGGCAAGACTGTCTCGCTCTACACCGACCTGAATATCCTCAATACCGTGGA
CATCAATATTTCCACCGCCGAAGATCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTGAACCCCCGCCAGG
GACTGGACTTTGCCCAGGCCCTGCGCTCCTTCCTGCGTCAGGACCCGGACGTGATCATGGTGGGAGAGATCCGCGACCTG
GAAACGGCGGAAATCGCCATCAAGGCAGCCCAGACCGGGCACATGGTGCTCTCCACCCTGCACACCAACAGTGCCGCGGA
AACCCTGATCCGCTTGCAGAACATGGGCATCCCCGGCTTCAACATCGCCACGGCGGTCCACCTGATCATCGCCCAGCGCC
TGGCGCGAAAACTGTGCAACCAATGCAAGAAGGCCACGGAGATTCCCGAGGAAACCCTGCTCAAGGAAGGCTTCCCCAAG
GAACGCATCGGCTCGTTCACGATCTATGAGCCGGTCGGTTGTGAACAGTGCAACAGTGGCTACAAAGGACGCGTGGGGGT
TTACGAAGTGGTGAAAAATACGCCCGAACTGCAACGGCTGATCATGGCCGAAGGCAATTCCCTGGAAATCGATCTGCAAA
TGCGCAAGGACGGGTTCAACGACCTGCGCACTTCGGGGTTACTGAAAGTGATGCAAGGGGTGACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.801

99.647

0.576
  pilB Acinetobacter baumannii D1279779

57.319

100

0.574
  pilB Legionella pneumophila strain ERS1305867

55.026

100

0.551
  pilB Vibrio cholerae strain A1552

50.177

100

0.502
  pilF Neisseria gonorrhoeae MS11

48.49

99.47

0.482
  pilB Vibrio campbellii strain DS40M4

48.142

99.823

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

48.82

97.35

0.475