Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LOY64_RS02280 Genome accession   NZ_CP102177
Coordinates   483163..485235 (+) Length   690 a.a.
NCBI ID   WP_024779905.1    Uniprot ID   -
Organism   Pseudomonas corrugata strain B21-055     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 478163..490235
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY64_RS02260 (LOY64_02260) comM 480375..481439 (+) 1065 WP_024779909.1 pilus assembly protein PilM Machinery gene
  LOY64_RS02265 (LOY64_02265) - 481439..482005 (+) 567 WP_024779908.1 PilN domain-containing protein -
  LOY64_RS02270 (LOY64_02270) pilO 482002..482625 (+) 624 WP_024779907.1 type 4a pilus biogenesis protein PilO -
  LOY64_RS02275 (LOY64_02275) - 482622..483149 (+) 528 WP_024779906.1 pilus assembly protein PilP -
  LOY64_RS02280 (LOY64_02280) pilQ 483163..485235 (+) 2073 WP_024779905.1 type IV pilus secretin PilQ Machinery gene
  LOY64_RS02285 (LOY64_02285) aroK 485240..485758 (+) 519 WP_024779904.1 shikimate kinase AroK -
  LOY64_RS02290 (LOY64_02290) aroB 485907..487007 (+) 1101 WP_024779903.1 3-dehydroquinate synthase -
  LOY64_RS02295 (LOY64_02295) - 487018..488583 (+) 1566 WP_024779902.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74769.02 Da        Isoelectric Point: 5.2967

>NTDB_id=715261 LOY64_RS02280 WP_024779905.1 483163..485235(+) (pilQ) [Pseudomonas corrugata strain B21-055]
MNRIFTALGVSLWIALMSPMVLGASLKTLDVAALPGDRVELKLTFDGPPPAPHGYTTEQPARIALDLPGVTNQLPRKTQD
LGSGNARSATVVEAGDRTRLIIGLTQLVPYNTRIEGNNLFVVVGKGAEPGTAAQVPRPAAAGVMPARSKAPAGRAIRGVD
FQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATISIEPGGTFD
YSTYQTDNKLTVSVRPMTVEDLRKRDTDRAAYKGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPW
DQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKAD
ERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNQGNWNSSGVTNGS
STTIGTPGSTSTNQPFVDMGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
ASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDLVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=715261 LOY64_RS02280 WP_024779905.1 483163..485235(+) (pilQ) [Pseudomonas corrugata strain B21-055]
ATGAACAGGATTTTTACTGCCCTCGGTGTTTCGCTATGGATAGCGCTGATGTCACCGATGGTTCTTGGGGCAAGCCTGAA
GACGCTGGATGTCGCGGCTTTGCCAGGGGATCGTGTCGAGTTGAAGCTGACTTTCGACGGGCCACCACCCGCCCCCCATG
GCTACACCACCGAACAACCGGCGCGCATTGCCCTGGACCTGCCTGGGGTGACCAACCAGCTGCCGCGCAAGACCCAGGAC
CTTGGCAGCGGCAATGCGCGCAGCGCCACGGTGGTCGAGGCGGGGGACCGCACCCGGCTGATCATCGGCCTCACTCAGTT
GGTGCCGTACAACACTCGGATCGAGGGTAATAACCTGTTCGTGGTAGTCGGCAAGGGCGCGGAGCCCGGGACTGCCGCGC
AAGTCCCGCGTCCGGCGGCTGCCGGCGTGATGCCTGCCAGAAGCAAGGCGCCGGCGGGCCGGGCCATTCGTGGCGTGGAT
TTCCAGCGCGGGACCCAGGGCGAAGGCAACGTAGTGATCGACCTATCGGACCCTTCCATCGCGCCGGACATCCAGGAGCG
GGAAGGCAAGATCATCCTCAACTTCGCCAGGACCCAATTGCCTGAACCACTGCGGGTACGCCTGGACGTCAAGGACTTCG
CCACCCCGGTGCAGTTCGTCAATGCCAGTGCCGGCTCCGACCGGGCCACTATCAGCATCGAGCCTGGCGGTACCTTCGAT
TACTCGACCTACCAGACCGACAATAAACTGACCGTCAGCGTCCGACCCATGACGGTCGAAGACTTGCGCAAGCGCGACAC
TGACCGTGCCGCCTACAAAGGTGAAAAGCTGTCCCTGAATTTCCAGGACATCGAAGTGCGCTCGGTCCTGCAACTGATTG
CCGATTTCACCAACCTGAACCTGGTGGCAAGCGACACGGTGCAGGGCGGTATTACCTTGCGGTTGCAGAATGTTCCATGG
GATCAGGCGCTGGATCTGGTGCTGAAGACCAAGGGACTGGACAAGCGCAAGGTCGGCAACGTGTTGCTGGTAGCCCCTGC
CGATGAGATTGCGGCTCGCGAGCGCCAGGAGCTGGAATCCCAGAAACAGATTGCCGACCTGGCGCCGCTGCGGCGAGAGT
TGTTGCAGGTCAACTATGCGAAGGCGGCGGATATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCCGAGGCAAAGGCCGAT
GAGCGCGGCACGATCACGGTGGATGAGCGAACCAACAACATCATTGCCTACCAGACTCAGGACCGGCTCGACGAATTGCG
CCGGATCGTGGCCCAGCTGGATATCCCGGTGCGTCAGGTGATGATCGAGGCCAGGATCGTCGAGGCCAACGTCGATTACG
ACAAAAGCCTGGGTGTGCGTTGGGGCGGCTCGGTGCAGAACCAGGGCAACTGGAACTCCTCGGGTGTGACCAATGGCAGT
TCCACCACCATCGGCACACCGGGCAGCACCAGCACCAACCAGCCATTCGTCGACATGGGCGCGTCGGGCAATACCTCGGG
TATTGGCATTGCGTTCATCACTGACAATGTGCTGCTGGATCTGGAGCTGACGGCCATGGAGAAAACCGGCAACGGGGAAA
TCGTCTCCCAGCCCAAGGTGGTGACTTCGGACAAGGAGACCGCGAAAATCCTCAAGGGCACAGAAATTCCCTACCAGGAA
GCCAGTTCCAGTGGCGCGACCTCGGTGTCGTTCAAGGAAGCCTCGCTGTCGCTGGAGGTAACCCCGCAGATCACGCCGGA
CAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAGGTGCAGGATGTGCCACCGATCA
AGAAGAACGAAGTCAACGCGAAGGTGCTGGTCAATGACGGCGAGACCATTGTGATTGGCGGGGTTTTCTCAAATACTCAG
AGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATCTGGTTTCGGA
GAAAAAATCCGAGCTGCTGGTATTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-121)

Secretin and TonB N terminus short domain (STN)

(300-346)

Bacterial type II/III secretion system short domain (Secretin_N)

(374-439)

Bacterial type II and III secretion system protein (Secretin)

(525-681)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.727

100

0.754
  comQ Acinetobacter baylyi ADP1

41.185

100

0.413
  pilQ Acinetobacter baumannii strain A118

38.378

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.378

100

0.412
  pilQ Legionella pneumophila strain ERS1305867

38.988

97.391

0.38
  pilQ Legionella pneumophila strain Lp02

38.897

97.246

0.378